Loss of heterochromatin protein 1 (HP1) chromodomain function in mammalian cells by intracellular antibodies causes cell death

Journal of Cell Science

Volumn 115, Issue 9, 2002, Pages 1803-1813

  Filesi, Ilaria ^a   Cardinale, Alessio ^a   van der Sar, Sjaak ^a   Cowell, Ian G ^a   Singh, Prim B ^a   Biocca, Silvia ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Apoptosis; Chromodomain; Heterochromatin; HP1 proteins; Intracellular antibodies; scFv fragments

Indexed keywords

BENZYLOXYCARBONYLASPARTYLGLUTAMYLVALYLASPARTYL FLUOROMETHYL KETONE; CASPASE INHIBITOR; CELL ANTIBODY; CHROMOSOME PROTEIN; EPITOPE; HETEROCHROMATIN PROTEIN 1; PROTEIN P53; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG; VEGETABLE PROTEIN; DIAGNOSTIC AGENT; ENZYME INHIBITOR; HETEROCHROMATIN SPECIFIC NONHISTONE CHROMOSOMAL PROTEIN HP 1; HETEROCHROMATIN-SPECIFIC NONHISTONE CHROMOSOMAL PROTEIN HP-1; LIPOCORTIN 5; NONHISTONE PROTEIN; PEPTIDE FRAGMENT;

ANIMAL CELL; ANTIGEN RECOGNITION; ARTICLE; CELL DEATH; CELL STRUCTURE; CELL SURVIVAL; COMPLEX FORMATION; CONTROLLED STUDY; FIBROBLAST; HETEROCHROMATIN; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; INCUBATION TIME; MACROMOLECULE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN LOCALIZATION; PROTEIN MOTIF; AMINO ACID SEQUENCE; ANIMAL; CELL CULTURE; CELL NUCLEUS; CELL STRAIN 3T3; CELL STRAIN COS1; CHROMATIN; CYTOLOGY; DRUG EFFECT; EUKARYOTIC CELL; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; MAMMAL; METABOLISM; MOLECULAR CLONING; MOUSE; MUTATION; PHENOTYPE; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; SERODIAGNOSIS;

3T3 CELLS; AMINO ACID SEQUENCE; ANIMAL; ANNEXIN V; CELL DEATH; CELL NUCLEUS; CHROMATIN; CHROMOSOMAL PROTEINS, NON-HISTONE; CLONING, MOLECULAR; COS CELLS; ENZYME INHIBITORS; EPITOPES; EUKARYOTIC CELLS; HUMAN; MACROMOLECULAR SYSTEMS; MAMMALS; MICE; MUTATION; PEPTIDE FRAGMENTS; PHENOTYPE; PRECIPITIN TESTS; PROTEIN BINDING; PROTEIN P53; PROTEIN STRUCTURE, TERTIARY; SUPPORT, NON-U.S. GOV'T; TUMOR CELLS, CULTURED; ANIMALS; ANNEXIN A5; HUMANS; MACROMOLECULAR SUBSTANCES; TUMOR SUPPRESSOR PROTEIN P53;

EID: 0036558280     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (60)

1. Functional mammalian homologues of the Drosophila PEV-modifier Su(var)3-9 encode centromere-associated proteins which complex with the heterochromatin component
2. The chromo shadow domain, a second chromo domain in heterochromatin-binding protein 1, HP1
3. INCENP centromere and spindle targeting: Identification of essential conserved motifs and involvement of heterochromatin protein HP1
4. Structure of the chromatin binding (chromo) domain from mouse modifier protein 1
5. Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain
6. Assembly of combinatorial antibody libraries on phage surface: The gene III site
7. Intracellular immunization: Antibody targeting to subcellular compartments
8. Intracellular expression of anti p21ras single chain Fv fragments inhibits meiotic maturation of Xenopus oocytes
9. Redox state of single-chain Fv fragments targeted to endoplasmic reticulum, cytosol and mitochondria
10. The structure of mouse HP1 suggests a unique mode of single peptide recognition by the shadow chromo dimer
11. Aggresome formation by anti-Ras intracellular scFv fragments: The fate of the antigen-antibody complex
12. Chromo-domain proteins: Linking chromatin structure to epigenetic regulation
13. Genetic mechanism of tumor suppression by the human p53 gene
14. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
15. Mutation in a heterochromatin-specific chromosomal protein is associated with suppression of position effect variegation in Drosophila melanogaster
16. Caspase inhibitors
17. The heterochromatin protein 1 prevents telomere fusions in Drosophila
18. Heterochromatin protein 1 modifies mammalian PEV in a dose-and chromosomal-context-dependent-manner
19. Homeogene expression patterns ad chromosomal imprinting
20. Rules to remodel by: What drives nuclear envelope disassembly and reassembly during mitosis?
21. Diverse and dynamic functions of the Sir silencing complex
22. The contribution of contact and non-contact of antibody in the affinity of binding antigen. The interaction of mutant D1:3 antibodies with lysozyme
23. Position-effect variegation after 60 years
24. M32, a murine homologue of Drosophila heterochromatin protein 1 (HP1) localises to euchromatin within interphase nuclei ad is largely excluded from constitutive heterochromatin
25. The murine heterochromatin protein M31 is associated with the chromocenter in round spermatids and is a component of mature spermatozoa
26. Identification of a nonhistone chromosomal protein associated with heterochromatin in Drosophila melanogaster and its gene
27. Mammalian chromodomain proteins: Their role in genome organization and expression
28. Heterochromatin protein 1 is required for correct chromosome segregation in Drosophila embryos
29. Dynamic associations of heterochromatin protein 1 with the nuclear envelope
30. Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system
31. Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins
32. p53, the cellular gatekeeper for growth and division
33. Diverting a protein from its cellular location by intracellular antibodies. The case of p21Ras
34. Intracellular expression of single-chain variable fragments to inhibit early stages of the viral life cycle by targeting human immunodeficiency virus type 1 integrase
35. Control of apoptosis and mitotic spindle checkpoint by survivin
36. Pleiotropic cell-division defects and apoptosis induced by interference with survivin function
37. Structure-function analysis of SUV39H1 reveals a dominant role in heterochromatin organization, chromosome segregation, and mitotic progression
38. Inhibition of HIV-1 Tat-mediated LTR transactivation and HIV-1 infection by anti-Tat single-chain intrabodies
39. Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells
40. M31, a murine homologue of Drosophila HP1, is concentrated in the XY body during spermatogenesis
41. Cloning immunoglobulin variable domains for expression by the polymerase chain reaction
42. Imprinting a determined state into the chromatin of Drosophila
43. The polycomb protein shares a homologues domain with a heterochromatin-associated protein of Drosophila
44. Chromatin complexes regulating gene expression in Drosophila
45. Targeting vectors for intracellular immunisation
46. Regulation of chromatin structure by site-specific histone H3 methyltransferases
47. Position-effect variegation and chromatin proteins
48. High-affinity recombinant phage antibodies to the pan-carcinoma marker ephitelial glycoprotein-2 for tumor targeting
49. KAP-1 corepressor protein interacts and colocalizes with heterochromatic and euchromatic HP1 proteins: A potential role for Kruppel-associated box zinc finger proteins in heterochromatin-mediated genes silencing
50. Exploiting recombination in single bacteria to make large phage libraries
51. Chromobox genes and the molecular mechanisms of cellular determination
52. A sequence motif found in Drosophila heterochromatin protein is conserved in animals and plants
53. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
54. Human Ku70 interacts with heterochromatin protein 1 alpha
55. Towards an understanding of position-effect variegation
56. Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack
57. M31 and macroH2A1.2 localisation during meiosis
58. Conservation of heterochromatin Protein 1 function
59. A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatin