Versatility of analytical capabilities of laser scanning cytometry (LSC)

Clinical and Applied Immunology Reviews

Volumn 2, Issue 2, 2002, Pages 75-92

  Grabarek, Jerzy ^a,b   Darzynkiewicz, Zbigniew ^a  

^a NEW YORK MEDICAL COLLEGE   (United States)

^b POMERANIAN MEDICAL UNIVERSITY   (Poland)

Author keywords

Apoptosis; Cell cycle; Cytometry; Cytoplasm; Enzyme kinetics; Fluorescence; Micronucleus; Nucleolus; Nucleus

Indexed keywords

CELL NUCLEUS DNA; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; PROTEIN BAX; PROTEIN P53;

ANALYTIC METHOD; APOPTOSIS; CELL NUCLEUS; CELL TYPE; CELLULAR DISTRIBUTION; CHROMATIN CONDENSATION; CLONOGENESIS; CONTROLLED STUDY; CYTOFLUOROMETRY; CYTOPLASM; ENZYME KINETICS; FLOW CYTOMETRY; FLUORESCENCE ANALYSIS; FLUORESCENCE IN SITU HYBRIDIZATION; IMAGING SYSTEM; IMMUNOCHEMISTRY; IMMUNOCYTOCHEMISTRY; IMMUNOPHENOTYPING; LASER MICROSCOPY; MITOSIS; MORPHOMETRICS; MUTAGENICITY; NUCLEOLUS; PROGENY; REVIEW; SEQUENTIAL ANALYSIS; TISSUE SECTION; TOPOGRAPHY;

EID: 0036555827     PISSN: 15291049     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1529-1049(01)00043-5     Document Type: Review

References (76)

1. Kamentsky LA, Kamentsky LD. Microscope-based multiparameter laser scanning cytometer yielding data comparable to flow cytometry data. Cytometry 1991;12:381-7.
2. Kamentsky LA, Burger DE, Gershman RJ, Kamentsky LD, Luther E. Slide-based laser scanning cytometry. Acta Cytol 1997;41:123-43.
3. Kamentsky LA. Laser scanning cytometry. Meth Cell Biol 2001;63:51-83.
4. Darzynkiewicz Z, Bedner E, Li X, Gorczyca W, Darzynkiewicz Z. Laser-scanning cytometry: a new instrumentation with many applications. Exp Cell Res 1999;249:1-12.
5. Luther E, Kamentsky LA. Resolution of mitotic cells using laser scanning cytometry. Cytometry 1996;23: 272-8.
6. Kawasaki M, Sasaki K, Satoh T, Kurose A, Kamada T, Furuya T, et al. Laser scanning cytometry (LSC) allows detailed analysis of the cell cycle in PI stained human fibroblasts (TIG-7). Cell Prolif 1997;30:139-47.
7. Juan G, Traganos F, James WM, Ray MJ, Roberge M, Sauve DM, et al. Histone H3 phosphorylation and expression of cyclins A and B1 measured in individual cells during their progression through G2 and mitosis. Cytometry 1998;21:1-8.
8. Gorczyca W, Melamed MR, Darzynkiewicz Z. Laser scanning cytometer (LSC) analysis of fraction of labeled mitoses (FLM). Cell Prolif 1996;29:539-47.
9. Quastler H, Sherman FG. Cell population kinetics in the intestinal epithelium of mouse. Exp Cell Res 1959; 17:420-38.
10. Schmid TE, Attia S, Baumargartner A, Nuesse M, Adler ID. Effect of chemicals on the duration of male meiosis in mice detected with laser scanning cytometry. Mutagenesis 2001;16:339-43.
11. Darzynkiewicz Z, Juan G, Li X, Gorczyca W, Murakami T, Traganos F. Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis). Cytometry 1997;27:1-20.
12. Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, et al. Features of apoptotic cells measured by flow cytometry. Cytometry 1992;13:795-808.
13. Furuya T, Kamada T, Murakami T, Kurose A, Sasaki K. Laser scanning cytometry allows detection of cell death with morphological features of apoptosis in cells stained with PI. Cytometry 1997;29:173-7.
14. Bedner E, Li X, Gorczyca W, Melamed MR, Darzynkiewicz Z. Analysis of apoptosis by laser scanning cytometry. Cytometry 1999;35:181-95.
15. Bedner E, Burfeind P, Gorczyca W, Melamed MR, Darzynkiewicz Z. Laser scanning cytometry distinguishes lymphocytes, monocytes and granulocytes by differences in their chromatin structure. Cytometry 1997;29:191-6.
16. Wolter KG, Hsu IT, Smith CL, Hechusthan A, Xi XG, Youle RJ. Movement of Bax from the cytosol to mitochondria during apoptosis. J Cell Biol 1997;139:1281-92.
17. Zamzani N, Brenner C, Marzo I, Susin SA, Kroemer G. Subcellular and submitochondrial mode of action of Bcl-2-like oncoproteins. Oncogene 1998;16:2265-82.
18. Bedner E, Li X, Kunicki J, Darzynkiewicz Z. Translocation of Bax to mitochondria during apoptosis measured by laser scanning cytometry. Cytometry 2000;41:83-8.
19. Baldwin Jr. AS. The NF-ΚB and IΚB proteins: new discoveries and insights. Annu Rev Immunol 1996;14: 649-81.
20. Deptala A, Bedner E, Gorczyca W, Darzynkiewicz Z. Activation of nuclear factor kappa B (NF-ΚB) assayed by laser scanning cytometry (LSC). Cytometry 1998;33:376-82.
21. Mercie P, Belloc F, Biblou-Nabera C, Barthe C, Provost A, Renard M, et al. Comparative methodologic study on NFΚB activation in cultured endothelial cells. J Lab Clin Med 2000;136:402-11.
22. WAF1, and apoptosis in relation to cell cycle phase of MCF-7 cells treated with camptothecin. Int J Oncol 1999;15:861-71.
23. Kawamura K, Kobayashi Y, Tanaka T, Ikeda R, Fujikawa-Yamamoto K, Suzuki K. Intranuclear localization of proliferating cell nuclear antigen during the cell cycle in renal cell carcinoma. Anal Quant Cytol Histol 2000;22:107-13.
24. Styles JA, Clark H, Festing MFW, Rew DA. Automation of mouse micronucleus genotoxicity assay by laser scanning cytometry. Cytometry 2001;44:153-5.
25. Smolewski P, Ruan Q, Vellon L, Darzynkiewicz Z. The micronuclei assay by laser scanning cytometry. Cytometry 2001;45:19-26.
26. Kamentsky LA, Kamentsky LD, Fletcher JA, Kurose A, Sasaki K. Methods for automatic multiparameter analysis of fluorescence in situ hybridized specimens with laser scanning cytometer. Cytometry 1997;27: 117-25.
27. Kobayashi Yesato K, Oga A, Sasaki K. Detection of 20q13 gain by dual-color FISH in breast cancers. Anticancer Res 2000;20:531-5.
28. Hashimoto Y, Oga A, Okami K, Imate Y, Yamashita Y, Sasaki K. Relationship between cytogenetic aberrations by CGH coupled with tissue microdissection and DNA ploidy by laser scanning cytometry in head and neck squamous cell carcinoma. Cytometry 2000;40:161-6.
29. Harada K, Nishizaki T, Kubota H, Harada K, Suzuki M, Sasaki K. Distinct primary central nervous system lymphoma defined by comparative genomic hybridization and laser scanning cytometry. Cancer Genet Cytogenet 2001;125:147-50.
30. Baumgartner A, Schmid TE, Maers HK, Adler ID, Tarnok A, Nuesse M. Automated evaluation of frequencies of aneuploid sperm by laser-scanning cytometry (LSC). Cytometry 2001;44:156-60.
31. Juan G, Cordon-Cardo C. Intranuclear compartmentalization of cyclin E during the cell cycle: disruption of the nucleoplasm-nucleolar shuttling of cyclin E in bladder cancer. Cancer Res 2001;61:1220-26.
32. Gorczyca W, Smolewski P, Ardelt B, Ita M, Melamed MR, Darzynkiewicz Z. Morphometry of nucleoli and expression of nucleolin analyzed by laser scanning cytometry in mitogenically stimulated lymphocytes. Cytometry 2001;45:206-213.
33. Bedner E, Ruan Q, Chen S, Kamentsky LA, Darzynkiewicz Z. Multiparameter analysis of progeny of individual cells by laser scanning cytometry. Cytometry 2000;40:271-9.
34. Clatch RJ, Foreman JR, Walloch JL. Simplified immunophenotypic analysis by laser scanning cytometry. Cytometry 1998;34:3-16.
35. Clatch RJ, Foreman JR. Five-color immunophenotyping plus DNA content analysis by laser scanning cytometry. Cytometry 1998;34:36-8.
36. Clatch RJ, Walloch JL. Multiparameter immunophenotypic analysis of fine needle aspiration biopsies and other hematologic specimens by laser scanning cytometry. Acta Cytol 1997;41:109-22.
37. Clatch RJ. Immunophenotyping of hematological malignancies by laser scanning cytometry. Meth Cell Biol 2001;64:313-42.
38. Bedner E, Melamed MR, Darzynkiewicz Z. Enzyme kinetic reactions and fluorochrome uptake rates measured in individual cells by laser scanning cytometry (LSC). Cytometry 1998;33:1-9.
39. Kerr JFR, Wyllie AH, Curie AR. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 1972;26:239-57.
40. Bedner E, Halicka HD, Cheng W, Salomon T, Deptala A, Gorczyca W, et al. High affinity binding of fluorescein isothiocyanate to eosinophils detected by laser scanning cytometry: a potential source of error in analysis of blood samples utilizing fluorescein conjugated reagents in flow cytometry. Cytometry 1999;36:77-82.
41. Bedner E, Burfeind P, Hsieh T-C, Wu JM, Augero-Rosenfeld M, Melamed MR, et al. Cell cycle effects and induction of apoptosis caused by infection of HL-60 cells with human granulocytic ehrlichiosis (HGE) pathogen measured by flow and laser scanning cytometry (LSC). Cytometry 1998;33:47-55.
42. Darzynkiewicz Z, Bedner E, Traganos F, Murakami T. Critical aspects in the analysis of apoptosis and necrosis. Human Cell 1998;11:3-12.
43. Darzynkiewicz Z, Bedner E, Traganos F. Difficulties and pitfalls in analysis of apoptosis. Meth Cell Biol 2001;63:527-46.
44. Li X, Melamed MR, Darzynkiewicz Z. Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color. Exp Cell Res 1996;222:28-37.
45. Darzynkiewicz Z, Bedner E. Analysis of apoptotic cells by flow- and laser scanning- cytometry. Meth Enzymol 2000;322:18-39.
46. Darzynkiewicz Z, Bedner E, Burfeind P, Traganos F. Analysis of apoptosis by flow and laser scanning cytometry. In: Zhu L, Chun J, editors. Apoptosis. Detection and assay methods. BioTechniques Books Series Vol. 2, Eaton Publishing; Natick, MA 1998. p. 75-92.
47. Gorczyca W, Bedner E, Burfeind P, Darzynkiewicz Z, Melamed MR. Analysis of apoptosis by laser-scanning cytometry. Mod Pathol 1988;11:1052-8.
48. Abdel-Moneim L, Melamed MR, Darzynkiewicz Z, Gorczyca W. Proliferation and apoptosis in solid tumors. Analysis by laser scanning cytometry. Anal Quant Cytol Histol 2000;22:393-7.
49. Zabaglo L, Ormerod MG, Dowsett M. Measurement of markers for breast cancer in model system using laser scanning cytometry. Cytometry 2000;41:166-71.
50. Darzynkiewicz Z, Li X, Bedner E. Use of flow and laser-scanning cytometry in analysis of cell death. Meth Cell Biol 2001;66:69-109.
51. Bedner E, Smolewski P, Amstad P, Darzynkiewicz Z. Activation of caspases measured in situ by binding of fluorochrome-labeled inhibitors of caspases (FLICA): correlation with DNA fragmentation. Exp Cell Res 2000;259:308-15.
52. Smolewski P, Bedner E, Du L, Hsiech T-C, Wu JM, Phelps DJ, et al. Detection of caspases activation by fluorochrome-labeled inhibitors: multiparameter analysis by laser scanning cytometry. Cytometry 2001;44:73-82.
53. Halicka HD, Bedner A, Darzynkiewicz Z. Segregation of RNA and separate packaging of DNA and RNA in apoptotic bodies during apoptosis. Exp Cell Res 2000;260:248-56.
54. Petersen AB, Gniadecki R, Wulf HC. Laser scanning cytometry for comet assay analysis. Cytometry 2000; 39:10-5.
55. Kakino S, Sasaki K, Kurose A, Ito H. Intracellular localization of cyclin B1 during cell cycle in gliomas cells. Cytometry 1996;24:49-54.
56. Smolewski P, Grabarek J, Kamentsky LA, Darzynkiewicz Z. Bivariate analysis of cellular DNA versus RNA content by laser scanning cytometry using the product of signal subtraction (differential fluorescence) as a separate parameter. Cytometry 2001;45:73-8.
57. Li X, Darzynkiewicz Z. The Schrödinger's cat quandary in biology: integration of live cell functional assays with measurements of fixed cells in analysis of apoptosis. Exp Cell Res 1999;249:404-12.
58. Li X, Du L, Darzynkiewicz Z. During apoptosis of HL-60 and U-937 cells caspases are activated independently of dissipation of mitochondrial electrochemical potential. Exp Cell Res 2000;257:290-7.
59. Bedner E, Du L, Traganos F, Darzynkiewicz Z. Caffeine dissociates complexes between DNA and intercalating dyes: application for bleaching fluorochrome-stained cells for their subsequent restaining and analysis by laser scanning cytometry. Cytometry 2001;43:38-45.
60. Clatch RJ, Walloch JL, Foreman JR, Kamentsky LA. Multiparameter analysis of DNA content and cytokeratin expression in breast carcinoma by laser scanning cytometry. Arch Pathol Lab Med 1997;121:585-92.
61. Woltmann G, Ward RJ, Symon FA, Rew DA, Pavord ID, Wardlaw AJ. Objective quantitative analysis of eosinophils and bronchial epithelial cells in induced sputum by laser scanning cytometry. Thorax 1999;54: 124-30.
62. Wojcik EM, Saraga SA, Jin JK, Hendricks JB. Application of laser scanning cytometry for evaluation of DNA ploidy in routine cytologic specimens. Diagn Cytopathol 2001;24:200-5.
63. Gerstner A, Lafler W, Bootz F, Tarnok A. Immunophenotyping of peripheral blood by laser scanning cytometry. J Immunol Methods 2000;246:175-85.
64. Kamiya N, Yokose T, Kiyomatsu Y, Fahey MT, Kodama T, Mukai K. Assessment of DNA content in formalin-fixed, paraffin-embedded tissue of lung cancer by laser scanning cytometry. Pathol Int 1999;49:695-701.
65. Grace MJ, Xie L, Musco ML, Cui S, Gurnani M, DiGiacomo R, et al. The use of laser scanning cytometry to assess depth of penetration of adenovirus p53 gene therapy in human xenograft biopsies. Am J Pathol 1999; 155:1869-78.
66. Musco ML, Shijun C, Small D, Nodelman M, Sugarman B, Grace M. Comparison of flow cytometry and laser scanning cytometry for the intracellular evaluation of adenoviral infectivity and p53 protein expression in gene therapy. Cytometry 1998;33:290-6.
67. Rew DA, Reeve LJ, Wilson GD. Comparison of flow and laser scanning cytometry for the assay of cell proliferation in human solid tumors. Cytometry 1998;33:355-61.
68. Gorczyca W, Darzynkiewicz Z, Melamed MR. Laser scanning cytometry in pathology of solid tumors. A review. Acta Cytol 1997;41:98-108.
69. Gorczyca W, Sarode V, Melamed MR, Darzynkiewicz Z. Laser scanning cytometric analysis of cyclin B1 in primary human malignancies. Mod Pathol 1997;10:457-62.
70. Kawamura K, Tanaka T, Ikeda R, Fujikawa-Yamamoto K, Suzuki K. DNA ploidy analysis in urinary tract epithelial tumors by laser scanning cytometry. Anal Quant Cytol Histol 2000;22:26-30.
71. Gorczyca W, Bedner E, Burfeind P, Darzynkiewicz Z, Melamed MR. Analysis of apoptosis in solid tumors by laser scanning cytometry. Mod Pathol 1998;11:1-7.
72. Gorczyca W, Davidian M, Gherson J, Ashikari R, Darzynkiewicz Z, Melamed MR. Laser scanning cytometry quantification of estrogen receptors in breast cancer. Anal Quant Cytol Histol 1999;20:470-6.
73. Woltmann G, Wardlaw AJ, Rew DA. Image analysis enhancement of the laser scanning cytometer. Cytometry 1997;33:262-5.
74. Mora J, Cheung NK, Juan G, Illei P, Cheung I, Akram M, et al. Neuroblastic and Schwannian stromal cells of neuroblastoma are derived from a tumor progenitor cell. Cancer Res 2001;61:6892-8.
75. Tanke HJ, De Haas RR, Sagner G, Ganser M, van Gijlswijk RPM. Use of platinum coproporphyrin and delayed luminescence imaging to extend the number of targets FISH karyotyping. Cytometry 1998;33:453-9.
76. Smolewski P, Bedner E, Gorczyca W, Darzynkiewicz Z. Liquidless cell staining by dye diffusion from gels and analysis by laser scanning cytometry. Potential application at microgravity conditions in space. Cytometry 2001;44:355-60.