Multiparameter analysis of DNA content and cytokeratin expression in breast carcinoma by laser scanning cytometry

Archives of Pathology and Laboratory Medicine

Volumn 121, Issue 6, 1997, Pages 585-592

  Clatch, Richard J ^a   Walloch, Jami L ^a   Foreman, James R ^b   Kamentsky, Louis A ^a  

^a ADVOCATE CHRIST MEDICAL CENTER   (United States)

^b CompuCyte Corporation ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOKERATIN; DNA;

ARTICLE; BREAST CARCINOMA; BREAST EPITHELIUM; CELL CYCLE S PHASE; CYTOMETRY; DNA CONTENT; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; LABORATORY; LASER MICROSCOPY; MAJOR CLINICAL STUDY; PROGNOSIS; SCANNING ELECTRON MICROSCOPY; TECHNOLOGY;

BREAST NEOPLASMS; DNA, NEOPLASM; FEMALE; HUMANS; IMAGE CYTOMETRY; IMAGE INTERPRETATION, COMPUTER-ASSISTED; KERATINS; LASERS; PLOIDIES; REGRESSION ANALYSIS; SENSITIVITY AND SPECIFICITY; SOFTWARE; TUMOR MARKERS, BIOLOGICAL;

EID: 0030995504     PISSN: 00039985     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (39)

1. Bauer KD, Duque RE, Shankey TV. Clinical Flow Cytometry. Baltimore, Md: Williams & Wilkins; 1993.
2. Keren DF, Hanson CA, Hurtubise PE. Flow Cytometry and Clinical Diagnosis. Chicago, III: ASCP Press; 1993.
3. Coon JS, Weinstein RS. Diagnostic Flow Cytometry. Baltimore, Md: Williams & Wilkins; 1991.
4. Hedley DW. DNA Cytometry Consensus Conference: DNA flow Cytometry and breast cancer. Breast Cancer Res Treat. 1993;28:51-53.
5. Spyratos F. DNA content and cell cycle analysis by flow cytometry in clinical samples: application in breast cancer. Bio! Cell. 1993;78:69-72.
6. Batsakis JG, Sneige N, el-Naggar AK. Flow cytometric (DNA content and S-phase fraction) analysis of breast cancer. Cancer. 1993;71:2151-2153.
7. Kamentsky LA, Kamentsky LD. Microscope-based multiparameter laser scanning cytometer yielding data comparable to flow cytometry data. Cytometry. 1991;12:381-387.
8. Martin-Reay DC, Kamentsky LA, Weinberg DS, Hollister KA, Cibas ES. Evaluation of a new slide-based laser scanning cytometer for DNA analysis of tumors: comparison with flow cytometry and image analysis. Am J Clin Palhol. 1994;102: 432-438.
9. Sasaki K, Kurose A, Miura Y, Sato T, Ikeda E. DNA ploidy analysis by laser scanning cytometry (LSC) in colorectal cancers and comparison with flow cytometry. Cytometry. 1996;23:106-109.
10. Clatch RJ, Walloch IL, Zutter MM, Kamentsky LA. Immunophenotypic analysis of hematologic malignancy by laser scanning cytometry. Am J Clin Pathol 1996;105:744-755.
11. Vindelov LL, Christensen IJ, Nissen NI. A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA content analysis. Cytometry. 1983;3:323-327.
12. Sun T. Color Atlas/Text of Flow Cytometric Analysis of Hematologic Neoplasms. New York, NY: Igaku Shoin; 1993:206-211.
13. Knowles DM. Organization and operation of a hematopathology laboratory. In: Knowles DM, ed. Neoplaslic Hematopathology. Baltimore, Md: Williams & Wilkins; 1992:323-366.
14. Hedley DW, Friedlander ML, Taylor JW, Rugg CA, Musgrave EA. Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. I Histochem Cytochem. 1983;31:1333-1335.
15. Zarbo RJ, Brown RD, Linden MD, Torres FX, Nakhleh RE, Schultz DS. Rapid (one-shot) staining method for two-color multiparametric DNA flow cytometric analysis of carcinomas using staining for cytokeratin and leukocyte common antigen. Am J Clin Palhol. 1994;101:638-642.
16. Brown RD, Zarbo RJ, Linden MD, et al. Two-color multiparametric method for flow cytometric DMA analysis: standardization of spectral compensation. Am 1 Clin Pathol. 1994;101:630-637.
17. Kenyon NS, Schmittling RJ, Siiman O, Burshteyn A, Bolton WE. Enhanced assessment of DNA/proliferative index by depletion of tumor infiltrating leukocytes prior to monoclonal antibody gated analysis of tumor cell DNA. Cytometry. 1994; 16:175-183.
18. Frei JV, Martinez VJ. DNA flow cytomelry of fresh and paraffin-embedded tissue using cytokeratin staining. Mod Pathol. 1993;6:599-605.
19. Savarese A, Nuti M, Botti C, Cianfriglia M, Vecchione A, Zupi G. Sample preparation for bivariate flow cytometric analysis of breast tumor specimens. Anal Quant Cytol Histol. 1993;15:39-49.
20. Begg AC, Hofland I. Cell kinetic analysis of mixed populations using threecolor fluorescence flow cytometry. Cytometry. 199l;12:445-454.
21. Visscher DW, Wykes S, Zarbo RJ, Crissman JD. Multiparametric evaluation of flow cylometric synthesis phase fraction determination in dual-labelled breast carcinomas. Anal Quant Cytol Histol. 1991;13:246-252.
22. Zarbo RJ, Visscher DW, Crissman JD. Two-color multiparametric method for flow cytometric DNA analysis of carcinomas using staining for cytokeratin and leukocyte-common antigen. Anal Quant Cytol Histol. 1989;11:391-402.
23. Kimmig R, Kapsner T, Spelsberg H, Untch M, Hepp H. DNA cell-cycle analysis of cervical cancer by flow cytometry using simultaneous cytokeratin labelling for identification of tumour cells. J Cancer Res Clin Oncol. 1995;121: 107-114.
24. Park CH, Kimler BF. Tumor cell-selective flow cytomelric analysis for DNA content and cytokeratin expression of clinical tumor specimens by "cross-gating." Anticancer Res. 1994;14:29-36.
25. FreiJV, Rizkalla K, Martinez VJ. Proliferate cell indices measured by DNA flow cytometry in node-negative adenocarcinomas of breast: accuracy and significance in cytokeratin-stained archival specimens. Mod Pathol. 1994;7:925929.
26. Wingren S, Stal O, Sullivan S, Brisfors A, Nordenskjold B. S-phase fraction after gating on epithelial cells predicts recurrence in node-negative breast cancer. Int J Cancer. 1994;59:7-10.
27. Wingren S, Stal O, Carstensen J, Sun XF, Nordenskjold B. S-phase determination of immunoselected cytokeratin-containing breast cancer cells improves the prediction of recurrence. Breast Cancer Res Treat. 1994;29:179-187.
28. Wingren S, Stal O, Nordenskjold B. Flow cytometric analysis of S-phase fraction in breast carcinomas using gating on cells containing cytokeratin. Br J Cancer. 1994;69:546-549.
29. Visscher DW, Zarbo R), Jacobsen G, et al. Multiparametric deoxyribonucleic acid and cell cycle analysis of breast carcinomas by flow cytometry: clinicopathologic correlations. Lab Invest. 1990;62:370-378.
30. Visscher DW, Zarbo RJ, Sakr WA, Crissman JD. Flow cytometric DNA and cell cycle analysis of cytokeratin-labeled breast carcinomas: correlations with prognostic factors. Lab Im-est. 1989;60:102A. Abstract.
31. Huffman JL, Carin-Chesa P, Cay H, Whitmore VVF Jr, Melamed M. Flow cytometric identification of human bladder cells using a cytokeratin monoclonal antibody. Ann NY Acad Sci. 1986;468:302-315.
32. Sasaki K, Kurose A, Shibata X Matsuta M. Varying detection of PCNA in solid tumor cells: effects of fixation and detergent. J Hislotechnol. 1995;18:31-34.
33. Sasaki K, Kurose A, Uesugi N, Kalo A. Effects of cycloheximide on chromatin-bound and -unbound PCNA in HeLa cells. Oncology. 1995;52:419-421.
34. Gong J, Traganos F, Darzynkiewicz Z. Discrimination of G2 and mitotic cells by flow cytometry based on different expression of cyclins A and B1. fxp Cell Res. 1995;220:226-231.
35. Gong J, Bhatia U, Traganos F, Darzynkiewicz Z. Expression of cyclins A, D2 and D3 in individual normal mitogen stimulated lymphocytes and in MOLT-4 leukemic cells analyzed by multiparameter flow cytometry. Leukemia. 1995;9: 893-899.
36. Darzynkiewicz Z, Gong J, Traganos F. Analysis of DNA content and cyclin protein expression in studies of DNA ploidy, growth fraction, lymphocyte stimulation, and the cell cycle. Methods Cell Biol. 1994;41:421-435.
37. Beppu T, Ishida Y, Arai H, Wada T, Uesugi N, Sasaki K. Identification of S-phase cells with PCI 0 antibody to proliferating cell nuclear antigen (PCNA) by flow cytometric analysis. I Histochcm Cytochem. 1994;42:1177-1182.
38. Sasaki K, Kurose A, Ishida Y. Flow cytometric analysis of the expression of PCNA during the cell cycle in HeLa cells and effects of the inhibition of DNA synthesis on it. Cytometry. 1993;14:876-882.
39. Visscher DW, Wykes S, Kubus J, Crissman JD. Comparison of PCNA/cyclin immunohistochemistry with flow cytometric S-phase fraction in breast cancer. Breast Cancer Res Treat. 1992;22:111-118.