Caffeine dissociates complexes between DNA and intercalating dyes: Application for bleaching fluorochrome-stained cells for their subsequent restaining and analysis by laser scanning cytometry

Cytometry

Volumn 43, Issue 1, 2001, Pages 38-45

  Bedner, Elzbieta ^a,b   Du, Litong ^a   Traganos, Frank ^a   Darzynkiewicz, Zbigniew ^a  

^a NEW YORK MEDICAL COLLEGE   (United States)

^b POMERANIAN MEDICAL UNIVERSITY   (Poland)

Author keywords

Cell cycle; Chromatin; Intercalators; Laser scanning cytometry; Stacking

Indexed keywords

7 AMINODACTINOMYCIN; CAFFEINE; DYE; FLUOROCHROME; INTERCALATING AGENT; METHYLXANTHINE; PROPIDIUM IODIDE; SULFORHODAMINE B; 7-AMINOACTINOMYCIN D; DACTINOMYCIN; DNA; DRUG DERIVATIVE; FLUORESCENT DYE;

ARTICLE; BINDING AFFINITY; BLEACHING; CELL STRAIN HL 60; CHROMATIN; COMPLEX FORMATION; DNA BINDING; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; HUMAN CELL; INTERCALATION COMPLEX; LASER MICROSCOPY; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; STAINING; AGAR GEL ELECTROPHORESIS; CHEMICAL MODEL; KINETICS; LASER; METABOLISM; METHODOLOGY;

CAFFEINE; DACTINOMYCIN; DNA; ELECTROPHORESIS, AGAR GEL; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENT DYES; HL-60 CELLS; HUMANS; INTERCALATING AGENTS; KINETICS; LASERS; MODELS, CHEMICAL; PROPIDIUM; STAINING AND LABELING;

EID: 0034745262     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0320(20010101)43:1<38::AID-CYTO1017>3.0.CO;2-S     Document Type: Article

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