Chemical genomics in the global study of protein functions

Drug Discovery Today

Volumn 7, Issue 3, 2002, Pages 197-205

  Steven Zheng, X F ^a   Chan, Ting Fung ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 (2 AMINO 3 METHOXYPHENYL)CHROMONE; BENOMYL; LIGAND; TACROLIMUS; PROTEIN;

CELL MEMBRANE PERMEABILITY; CELL METABOLISM; CHEMICAL GENETICS; DRUG TARGETING; GENE EXPRESSION; GENE INTERACTION; GENE MAPPING; GENOMICS; NONHUMAN; PROTEIN ENGINEERING; PROTEIN FUNCTION; REVIEW; SIGNAL TRANSDUCTION; CHEMISTRY; CHROMOSOME MAP; GENE EXPRESSION REGULATION; HUMAN; PHYSIOLOGY;

CHEMISTRY; CHROMOSOME MAPPING; GENE EXPRESSION REGULATION; GENOMICS; HUMANS; LIGANDS; PROTEIN ENGINEERING; PROTEINS;

EID: 0036462476     PISSN: 13596446     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1359-6446(01)02118-3     Document Type: Review

References (71)

1. Genes are mutable units
2. RNAi and double-strand RNA
3. Chemical genetics resulting from a passion for synthetic organic chemistry
4. Chemical genetics: Exploring and controlling cellular processes with chemical probes
5. Chemical genetics: Ligand-based discovery of gene function
6. Chemical genetic approaches for the elucidation of signaling pathways
7. Sequence-specific DNA recognition by polyamides
8. Mevinolin: A highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme A reductase and a cholesterol-lowering agent
9. Regulation of protein kinase C
10. The yeast phosphatidylinositol kinase homolog TOR2 activates RHO1 and RHO2 via the exchange factor ROM2
11. TOR kinase domains are required for two distinct functions, only one of which is inhibited by rapamycin
12. Detoxification mechanisms and tumor cell resistance to anticancer drugs
13. Chemistry and biology of the immunophilins and their immunosuppressive ligands
14. Inhibition of the immune response by rapamycin, a new antifungal antibiotic
15. Lactacystin, a novel microbial metabolite, induces neuritogenesis of neuroblastoma cells
16. Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin
17. Target of rapamycin in yeast, TOR2, is an essential phosphatidylinositol kinase homolog required for G1 progression
18. Dominant missense mutations in a novel yeast protein related to mammalian phosphatidylinositol 3-kinase and VPS34 abrogate rapamycin cytotoxicity
19. RAFT1: A mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs
20. A mammalian protein targeted by G1-arresting rapamycin-receptor complex
21. Isolation of a protein target of the FKBP12-rapamycin complex in mammalian cells
22. Lactacystin, proteasome function, and cell fate
23. Rapamycin-FKBP specifically blocks growth-dependent activation of and signaling by the 70 kd S6 protein kinases
24. Rapamycin selectively inhibits interleukin-2 activation of p70 S6 kinase
25. The TOR signaling pathway controls nuclear localization of nutrient-regulated transcriptional factors
26. Tripartite regulation of Gln3p by TOR, Ure2p and phosphatases
27. The TOR signaling cascade regulates gene expression in response to nutrients
28. Rapamycin-modulated transcription defines the subset of nutrient-sensitive signaling pathways directly controlled by the Tor proteins
29. Signalling-2000 and beyond
30. Unnatural ligands for engineered proteins: New tools for chemical genetics
31. Engineering unnatural nucleotide specificity for Rous sarcoma virus tyrosine kinase to uniquely label its direct substrates
32. Design of allele-specific inhibitors to probe protein kinase signaling
33. Controlling signal transduction with synthetic ligands
34. A versatile synthetic dimerizer for the regulation of protein-protein interactions
35. Target-oriented and diversity-oriented organic synthesis in drug discovery
36. New roles for structure in biology and drug discovery
37. Printing small molecules as microarrays and detecting protein-ligand interactions en masse
38. Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions
39. Printing proteins as microarrays for high-throughput function determination
40. Analysis of yeast protein kinases using protein chips
41. Genomics, gene expression and DNA arrays
42. Genomewide studies of histone deacetylase function in yeast
43. Functional discovery via a compendium of expression profiles
44. Drug target validation and identification of secondary drug target effects using DNA microarrays
45. The ubiquitin system
46. Evolution and function of ubiquitin-like protein-conjugation systems
47. Histone methylation versus histone acetylation: New insights into epigenetic regulation
48. Acetylation of histones and transcription-related factors
49. The meteoric rise of regulated intracellular proteolysis
50. The complexity of p53 modulation: Emerging patterns from divergent signals
51. Two-dimensional gel electrophoresis: A powerful method to elucidate cellular responses to toxic compounds
52. Mass spectrometry and proteomics
53. A thousand points of light: The application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics
54. Differential screening and mass mapping of proteins from premalignant and cancer cell lines using nonporous reversed-phase HPLC coupled with mass spectrometric analysis
55. Isoelectric focusing nonporous RP HPLC: A two-dimensional liquid-phase separation method for mapping of cellular proteins with identification using MALDI-TOF mass spectrometry
56. Continuous free-flow electrophoresis separation of cytosolic proteins from the human colon carcinoma cell line LIM 1215: A non two-dimensional gel electrophoresis-based proteome analysis strategy
57. Prefractionation techniques in proteome analysis
58. Quantitative analysis of complex protein mixtures using isotope-coded affinity tags
59. Enrichment analysis of phosphorylated proteins as a tool for probing the phosphoproteome
60. Detection of multiple proteins in an antibody-based protein microarray system
61. Immunophenotyping of leukemias using a cluster of differentiation antibody microarray
62. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis
63. High-throughput isolation of Caenorhabditis elegans deletion mutants
64. High-throughput reverse genetics: RNAi screens in Caenorhabditis elegans
65. The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes
66. Genome-wide mutant collections: Toolboxes for functional genomics
67. Genomic profiling of drug sensitivities via induced haploinsufficiency
68. Feedback control of mitosis in budding yeast
69. A chemical genomics approach toward understanding the global functions of TOR
70. Differential toxicities of anticancer agents among DNA repair and checkpoint mutants of Saccharomyces cerevisiae
71. Use of isogenic human cancer cells for high-throughput screening and drug discovery