Two-dimensional gel electrophoresis: A powerful method to elucidate cellular responses to toxic compounds

Toxicology

Volumn 160, Issue 1-3, 2001, Pages 129-138

  Möller, Anja ^a   Soldan, Michael ^a   Völker, Uwe ^b   Maser, Edmund ^a  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

^b MAX PLANCK INSTITUTE FOR TERRESTRIAL MICROBIOLOGY   (Germany)

Author keywords

Cytotoxicity; Daunorubicin; Pancreas carcinoma cell line; Stress response; Two dimensional gel electrophoresis

Indexed keywords

BIOLOGICAL MARKER; DAUNORUBICIN; HEAT SHOCK PROTEIN 60; TOXIC SUBSTANCE;

ANALYTIC METHOD; ARTICLE; CARCINOMA CELL; CELL FUNCTION; CONTROLLED STUDY; CYTOTOXICITY; DOSE RESPONSE; ENVIRONMENTAL EXPOSURE; HUMAN; HUMAN CELL; IMAGE ANALYSIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN MODIFICATION; REPRODUCIBILITY; RISK ASSESSMENT; RISK FACTOR; STRESS; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ADENOCARCINOMA; ANTIBIOTICS, ANTINEOPLASTIC; DAUNORUBICIN; DNA PRIMERS; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; PANCREATIC NEOPLASMS; PROTEOME; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TOXICITY TESTS; TUMOR CELLS, CULTURED; UP-REGULATION;

EID: 0035819883     PISSN: 0300483X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0300-483X(00)00443-1     Document Type: Article

References (30)

1. Proteome and proteomics: New technologies, new concepts, and new words
2. Isoelectric focusing in immobilized pH gradients: Principle, methodology and some applications
3. A rapid and sensitive method for the quantification of microgram quantities of proteins using the principle of dye-binding
4. The RNA polymerase II core subunit 11 interacts with keratin 19, a component of the intermediate filament proteins
5. Supervising the fold: Functional principles of molecular chaperones
6. The interacting RNA polymerase II subunits, hRPB11 and hRPB3, are coordinately expressed in adult human tissues and down-regulated by doxorubicin
7. Interaction specificity of the anthracyclines with deoxyribonucleic acid
8. The current state of two-dimensional electrophoresis with immobilized pH gradients
9. The anti-cancer drug cisplatin induces H25 in Ehrlich ascites tumor cells by a mechanism different from transcriptional stimulation influencing predominantly H25 translation
10. Drg-1 as a differentiation-related, putative metastatic suppressor gene in human colon cancer
11. The role of molecular chaperones in protein folding
12. Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein databases
13. The transcriptional program in the response of human fibroblasts to serum
14. Hsp 70 exerts its anti-apoptotic function downstream of caspase-3-like proteases
15. Protein identification by mass profile fingerprinting
16. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
17. Use of mass spectrometric molecular weight information to identify proteins in sequence databases
18. The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells
19. Protein disulfide isomerase: The multifunctional redox chaperone of the endoplasmic reticulum
20. High resolution two-dimensional electrophoresis of proteins
21. Identification of human myocardial proteins separated by two-dimensional electrophoresis using an effective sample preparation for mass spectrometry
22. Selected subunits of the cytosolic chaperonin associate with microtubules assembled in vitro
23. DNA topoisomerases as targets for chemotherapy
24. Improved and simplified in-gel sample application using reswelling of dry immobilized pH gradients
25. Quantitative monitoring of gene expression patterns with a complementary DNA microarray
26. Anthracyclines
27. The yeast homolog to mouse Tcp-1 affects microtubule-mediate processes
28. A novel gene which is up-regulated during colon epithelial cell differentiation and down-regulated in colorectal neoplasms
29. Protein disulfide isomerase assists protein folding as both an isomerase and a chaperone
30. Hsp 60 accelerates the maturation of pro-caspase-3 by upstream activator proteases during apoptosis