Use of isogenic human cancer cells for high-throughput screening and drug discovery

Nature Biotechnology

Volumn 19, Issue 10, 2001, Pages 940-945

  Torrance, Christopher J ^a   Agrawal, Vijai ^b   Vogelstein, Bert ^a   Kinzler, Kenneth W ^c  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^b Aral Biosynthetics   (United States)

^c JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; DRUG PRODUCTS; GENES; PROTEINS; TUMORS;

DRUG DISCOVERY;

BIOTECHNOLOGY;

4 (4 FLUOROPHENYL) 2 (4 METHYLSULFINYLPHENYL) 5 (4 PYRIDYL)IMIDAZOLE; ANTINEOPLASTIC AGENT; C 169A9; C 169B9; C 29A2; C 57D11; C 74D8; CYTIDINE DERIVATIVE; DEMETHOXYVIRIDIN; FLUORESCENT DYE; MITHRAMYCIN; NUCLEOSIDE ANALOG; PROTEIN; RAS PROTEIN; TRIPHENYLTETRAZOLIUM; UNCLASSIFIED DRUG; WORTMANNIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CANCER CELL; CARCINOGENESIS; COLON CANCER; CONTROLLED STUDY; CYTOTOXICITY; DRUG SCREENING; DRUG SPECIFICITY; EXPRESSION VECTOR; FEMALE; GENE DELETION; GENETIC RECOMBINATION; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; ONCOGENE K RAS; PRIORITY JOURNAL;

ANDROSTENES; ANIMALS; ANTINEOPLASTIC AGENTS; BACTERIAL PROTEINS; DRUG SCREENING ASSAYS, ANTITUMOR; FEMALE; GENE DELETION; GENES, RAS; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MICE; MICE, KNOCKOUT; MICE, NUDE; MUTAGENESIS; PLICAMYCIN; RECOMBINATION, GENETIC; SULFUR COMPOUNDS; TETRAZOLIUM SALTS; TUMOR CELLS, CULTURED;

EID: 0034795339     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt1001-940     Document Type: Article

References (17)

1. Activity of a specific inhibitor of the BCR-ABL tyrosine kinase in the blast crisis of chronic myeloid leukemia and acute lymphoblastic leukemia with the Philadelphia chromosome
2. The molecular and cellular biology of HER2/neu gene amplification/overexpression and the clinical development of herceptin (trastuzumab) therapy for breast cancer
3. Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae
4. Altered growth of human colon cancer cell lines disrupted at activated Ki-ras
5. Signal-transduction cascades as targets for therapeutic intervention by natural products
6. The G-C specific DNA binding drug, mithramycin, selectively inhibits transcription of the C-MYC and C-HA-Ras genes in regenerating liver
7. Sensitivity and specificity of triphenyl tetrazolium chloride in the gross diagnosis of acute myocardial infarcts
8. 2,3,5-Triphenyl tetrazolium chloride (TTC) reduction as exponential growth phase marker for mammalian cells in culture and for myeloma hybridization experiments
9. SB 203580 is a specific inhibitor of a MAP kinase homologue which is stimulated by cellular stresses and interleukin-1
10. Design and synthesis of potent, selective, and orally bioavailable tetrasubstituted imidazole inhibitors of p38 mitogen-activated protein kinases
11. Protocols for screening chemical agents and natural products against animal tumors and other biological systems
12. Green fluorescent protein (GFP): Applications in cell-based assays for drug discovery
13. Mechanism-based target identification and drug discovery
14. Understanding Ras: 'It ain't over 'til it's over'
15. Mouse genetics/genomics: An effective approach for drug target discovery and validation
16. Transgenic gene knock-outs: Functional genomics and therapeutic target selection
17. Gene targeting in human cells without isogenic DNA