Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA

European Journal of Biochemistry

Volumn 269, Issue 20, 2002, Pages 4981-4984

  Fatemi, Mehrnaz ^a   Hermann, Andrea ^a   Gowher, Humaira ^a   Jeltsch, Albert ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

DNA methylation; DNA methyltransferase; Dnmt1; Dnmt3a; Enzyme mechanism

Indexed keywords

DNA; DNA METHYLTRANSFERASE; DNA METHYLTRANSFERASE 1; DNA METHYLTRANSFERASE 3A; METHYL GROUP; UNCLASSIFIED DRUG;

ARTICLE; CPG ISLAND; DNA METHYLATION; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME PURIFICATION; IN VIVO STUDY; MODEL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

DNA (CYTOSINE-5-)-METHYLTRANSFERASE; DNA METHYLATION; SITE-SPECIFIC DNA METHYLTRANSFERASE (CYTOSINE-SPECIFIC);

STAPHYLOCOCCUS PHAGE 3A;

EID: 0036415141     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2002.03198.x     Document Type: Article

References (36)

1. Robertson, K.D. & Wolffe, A.P. (2000) DNA methylation in health and disease. Nat. Reviews Genetics 1, 11-19.
2. Jones, P.A. & Takai, D. (2001) The role of DNA methylation in mammalian epigenetics. Science 293, 1068-1070.
3. Jeltsch, A. (2002) Beyond Watson and Crick: DNA methylation and molecular enzymology of DNA methyltransferases. Chembiochem, in press.
4. Ferguson-Smith, A.C. & Surani, M.A. (2001) Imprinting and the epigenetic asymmetry between parental genomes. Science 293, 1086-1089.
5. Chao, W., Huynh, K.D., Spencer, R.J., Davidow, L.S. & Lee, J.T. (2002) CTCF, a candidate trans-acting factor for X-inactivation choice. Science 295, 345-347.
6. Li, E., Bestor, T.H. & Jaenisch, R. (1992) Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell 69, 915-926.
7. Okano, M., Bell, D.W., Haber, D.A. & Li, E. (1999) DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 99, 247-257.
8. Hsieh, C.L. (2000) Dynamics of DNA methylation pattern. Curr. Opin. Genet. Dev. 10, 224-228.
9. Wole, A.P., Jones, P.L. & Wade, P.A. (1999) DNA demethylation. Proc. Natl Acad. Sci. USA 96, 5894-5896.
10. Reik, W., Dean, W. & Walter, J. (2001) Epigenetic reprogramming in mammalian development. Science 293, 1089-1093.
11. Jones, P.A. & Vogt, P.K. (2000) DNA Methylation and Cancer. Springer Verlag, Heidelberg.
12. Baylin, S.B. & Herman, J.G. (2000) DNA hypermethylation in tumorigenesis: Epigenetics joins genetics. Trends Genet. 16, 168-174.
13. Warnecke, P.M. & Bestor, T.H. (2000) Cytosine methylation and human cancer. Curr. Opin. Oncol. 12, 68-73.
14. Cheng, X. (1995) Structure and function of DNA methyltransferases. Annu. Rev. Biophys. Biomol. Struct. 24, 293-318.
15. Bestor, T.H. (2000) The DNA methyltransferases of mammals. Hum. Mol. Genet. 9, 2395-2402.
16. Cheng, X. & Roberts, R.J. (2001) AdoMet-dependent methylation, DNA methyltransferases and base flipping. Nucl. Acids Res. 29, 3784-3795.
17. Pradhan, S., Bacolla, A., Wells, R.D. & Roberts, R.J. (1999) Recombinant human DNA (cytosine-5) methyltransferase. J. Biol. Chem. 274, 33002-33010.
18. Fatemi, M., Hermann, A., Pradhan, S. & Jeltsch, A. (2001) The activity of the murine DNA methyltransferase Dnmt1 is controlled by interaction of the catalytic domain with the N-terminal part of the enzyme leading to an allosteric activation of the enzyme after binding to methylated DNA. J. Mol. Biol. 309, 1189-1199.
19. Bacolla, A., Pradhan, S., Larson, J.E., Roberts, R.J. & Wells, R.D. (2001) Recombinant human DNA (cytosine-5) methyltransferase. III. Allosteric control, reaction order, and influence of plasmid topology and triplet repeat length on methylation of the fragile X CGG.CCG sequence. J. Biol. Chem. 276, 18605-18613.
20. Okano, M., Xie, S. & Li, E. (1998) Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases. Nature Genet. 19, 219-220.
21. Gowher, H. & Jeltsch, A. (2001) Enzymatic properties of recombinant Dnmt3a DNA methyltransferase from mouse: The enzyme modifies DNA in a non-processive manner and also methylates non-CpG sites. J. Mol. Biol. 309, 1201-1208.
22. Aoki, A., Suetake, I., Miyagawa, J., Fujio, T., Chijiwa, T., Sasaki, H. & Tajima, S. (2001) Enzymatic properties of de novo-type mouse DNA (cytosine-5) methyltransferases. Nucl. Acids Res. 29, 3506-3512.
23. Hsieh, C.-L. (1999) In vivo activity of murine de novo methyltransferases, Dnmt3a and Dnmt3b. Mol. Cell. Biol. 19, 8211-8218.
24. Hansen, R.S., Wijmenga, C., Luo, P., Stanek, A.M., Canfield, T.K., Weemaes, C.M. & Gartler, S.M. (1999) The DNMT3B DNA methyltransferase gene is mutated in the ICF immunodeficiency syndrome. Proc. Natl Acad. Sci. USA 96, 14412-14417.
25. Xu, G.-L., Bestor, T.H., Bourc'his, D., Hsieh, C.-L., Tommerup, N., Bugge, M., Hulten, M., Qu, X., Russo, J.J. & Viegas-Pequignot, E. (1999) Chromosome instability and immunodeficiency syndrome caused by mutations in a DNA methyltransferase gene. Nature 402, 187-191.
26. Roth, M. & Jeltsch, A. (2000) Biotin-Avidin microplate assay for the quantitative analysis of enzymatic methylation of DNA by DNA methyltransferases. Biol. Chem. 381, 269-272.
27. Kim, G.D., Ni, J., Kelesoglu, N., Roberts, R.J. & Pradhan, S. (2002) Co-operation and communication between the human maintenance and de novo DNA (cytosine-5) methyltransferases. EMBO J. 21, 4183-4195.
28. Lin, I.G., Han, L., Taghva, A., O'Brien, L.E. & Hsieh, C.L. (2002) Murine de novo methyltransferase dnmt3a demonstrates strand asymmetry and site preference in the methylation of DNA in vitro. Mol. Cell Biol. 22, 704-723.
29. Tollefsbol, T.O. & Hutchison, C.A.I. (1995) Mammalian DNA (cytosine-5-) methyltransferase expressed in Escherichia coli, purified and characterized. J. Biol. Chem. 270, 18543-18550.
30. Carotti, D., Funiciello, S., Palitti, F. & Strom, R. (1998) Influence of pre-existing methylation on the de novo activity of eukaryotic DNA methyltransferase. Biochemistry 37, 1101-1108.
31. Bacolla, A., Pradhan, S., Roberts, R.J. & Wells, R.D. (1999) Recombinant human DNA (cytosine-5) methyltransferase. II. Steady-state kinetics reveal allosteric activation by methylated DNA. J. Biol. Chem. 274, 33011-33019.
32. Flynn, J. & Reich, N. (1998) Murine DNA (cytosine-5-) methyl-transferase: Steady-state and substrate trapping analyses of the kinetic mechanism. Biochemistry 37, 15162-15169.
33. Qiu, C., Sawada, K., Zhang, X. & Cheng, X. (2002) The PWWP domain of mammalian DNA methyltransferase Dnmt3b defines a new family of DNA-binding fold. Nat. Struct. Biol. 9, 217-224.
34. Liang, G., Chan, M.F., Tomigahara, Y., Tsai, Y.C., Gonzales, F.A., Li, E., Laird, P.W. & Jones, P.A. (2002) Cooperativity between DNA methyltransferases in the maintenance methylation of repetitive elements. Mol. Cell Biol. 22, 480-491.
35. Bachman, K.E., Rountree, M.R. & Baylin, S.B. (2001) Dnmt3a and Dnmt3b are transcriptional repressors that exhibit unique localization properties to heterochromatin. J. Biol. Chem. 276, 32282-32287.
36. Rhee, I., Jair, K.W., Yen, R.W., Lengauer, C., Herman, J.G., Kinzler, K.W., Vogelstein, B., Baylin, S.B. & Schuebel, K.E. (2000) CpG methylation is maintained in human cancer cells lacking DNMT1. Nature 404, 1003-1007.