Enzymatic properties of recombinant Dnmt3a DNA methyltransferase from mouse: The enzyme modifies DNA in a non-processive manner and also methylates non-CpA sites

Journal of Molecular Biology

Volumn 309, Issue 5, 2001, Pages 1201-1208

  Gowher, Humaira ^a   Jeltsch, Albert ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

De novo methylation; DNA methylation; Enzyme specificity; Epigenetics; Gene regulation

Indexed keywords

DNA METHYLTRANSFERASE; METHYL GROUP; OLIGONUCLEOTIDE; RECOMBINANT ENZYME; RECOMBINANT PROTEIN;

ARTICLE; CATALYSIS; DNA METHYLATION; DNA MODIFICATION; ENZYME ACTIVITY; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE CONTROL; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; PROTEIN METHYLATION;

ESCHERICHIA COLI; MAMMALIA;

EID: 0035933337     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4710     Document Type: Article

References (31)

1. Cancer epigenetics comes of age
2. Dynamics of DNA methylation pattern
3. Methylation moves into medicine
4. Differential mRNA expression of the human DNA methyltransferases (DNMTs) 1, 3a and 3b during the G0/G1 to S phase transition in normal and tumor cells
5. CG and CNG methyltransferases in plants
6. Non-CpG methylation is prevalent in embryonic stem cells and may be mediated by DNA methyltransferase 3a
7. DNA modification by methyltransferases
8. Structure and function of DNA methyltransferases
9. DNA methyltransferases
10. Bacterial DNA methyltransferases
11. Eukaryotic DNA methyltransferases
12. The mojo of methylation
13. Dissecting de novo methylation
14. Eukaryotic DNA methyltransferases - Structure and function
15. Baculovirus-mediated expression and characterization of the full-length murine DNA methyltransferase
16. Recombinant human DNA (cytosine-5) methyltransferase
17. Recombinant human DNA (cytosine-5) methyltransferase. II. Steady-state kinetics reveal allosteric activation by methylated DNA
18. Murine DNA cytosine-C5 methyltransferase: Pre-steady- and steady-state kinetic analysis with regulatory DNA sequences
19. The activity of the murine DNA methyltransferase Dnmt1 is controlled by interaction of the catalytic domain with the N-terminal part of the enzyme leading to an allosteric activation of the enzyme after binding to methylated DNA
20. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality
21. De novo DNA cytosine methyltransferase activities in mouse embryonic stem cells
22. Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases
23. The human DNA methyltransferases (DNMTs) 1, 3a and 3b: Coordinate mRNA expression in normal tissues and overexpression in tumors
24. Chromosome instability and imunodeficiency syndrome caused by mutations in a DNA methyltransferase gene
25. The DNMT3B DNA methyltransferase gene is mutated in the ICF immunodeficiency syndrome
26. DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development
27. In vivo activity of murine de novo methyltransferases, Dnmt3a and Dnmt3b
28. Mammalian (cytosine-5) methyltransferases cause genomic DNA methylation and lethality in drosophila
29. Dependence of McrBC cleavage on distance between recognition elements
30. Molecular enzymology of the EcoRV DNA-(adenine-N6)-methyltransferase: Kinetics of DNA binding and bending, kinetic mechanism, and linear diffusion of the enzyme on DNA
31. Biotin-avidin microplate assay for the quantitative analysis of enzymatic methylation of DNA by DNA methyltransferases