The human Prp8 protein is a component of both U2- and U12-dependent spliceosomes

RNA

Volumn 5, Issue 7, 1999, Pages 893-908

  Luo, Hongbo R ^b   Moreau, Gilles A ^a,b   Levin, Natasha ^b   Moore, Melissa J ^b  

^a UNIVERSITY OF GENEVA   (Switzerland)

^b BRANDEIS UNIVERSITY   (United States)

Author keywords

Pre mRNA splicing; Prp8p; Spliceosome; U12 dependent spliceosome

Indexed keywords

NUCLEAR PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; HUMAN; INTRON; MOLECULAR CLONING; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SOUTHERN BLOTTING; SPLICEOSOME;

EID: 0033028397     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838299990520     Document Type: Article

References (69)

1. Achsel T, Ahrens K, Brahms H, Teigelkamp S, Luhrmann R. 1998. The human U5-220 kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein. Mol Cell Biol 18:6756-6766.
2. Adams MD, Rudner DZ, Rio DC. 1996. Biochemistry and regulation of pre-mRNA splicing. Curr Opin Cell Biol 8:331-339.
3. Altschul SF, Madden TL, Schärfer AA, Zhan J, Zhang Z, Miller W Lipman DJ. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res 25:3389-3402.
4. Anderson GJ, Bach M, Lührmann R, Beggs JD. 1989. Conservation between yeast and man of a protein associated with U5 small nuclear ribonucleoprotein. Nature 342:819-821.
5. Arning S, Grüter P, Bilbe G, Krämer A. 1996. Mammalian splicing factor SF1 is encoded by variant cDNAs and binds to RNA. RNA 2:794-810.
6. Bach M, Winkelmann G, Lührmann R. 1989. 20S small nuclear ribonucleoprotein U5 shows a surprisingly complex protein composition. Proc Natl Acad Sci USA 86:6038-6042.
7. Baker TA, Bell SP. 1998. Polymerases and the replisome: Machines within machines. Cell 92:295-305.
8. Baserga SJ, Steitz JA. 1993. The diverse world of small ribonucleoproteins. In: Gesteland R, Atkins J, eds. The RNA world. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press. pp 359-381.
9. Behrens S-E, Lührmann R. 1991. Immunoaffinity purification of a [U4/U6.U5] tri-snRNP from human cells. Genes & Dev 5:1439-1452.
10. Berget SM. 1995. Exon recognition in vertebrate splicing. J Biol Chem 270:2411-2414.
11. Black DL, Pinto AL 1989. U5 small nuclear ribonucleoprotein:RNA structure analysis and ATP-dependent interaction with U4/U6. Mol Cell Biol 9:335-3359.
12. Brown JD, Beggs JD. 1992. Roles of PRP8 protein in the assembly of splicing complexes. EMBO J 11:3721-3729.
13. Buratowski S. 1994. The basics of basal transcription by RNA polymerase II. Cell 77:1-3.
14. Burge CB, Padgett RA, Sharp PA. 1998. Evolutionary fates and origins of U12-type introns. Mol Cell 2:773-785.
15. Chiara MD, Gozani O, Bennett M, Champion-Arnaud P, Palandjian L, Reed R. 1996. Identification of proteins that interact with exon sequences, splice sites, and the branchpoint sequence during each stage of spliceosome assembly. Mol Cell Biol 16:3317-3326.
16. Chiara MD, Palandjian L, Kramer RF, Reed R. 1997. Evidence that U5 snRNP recognizes the 3′ splice site for catalytic step II in mammals. EMBO J 16:4746-4759.
17. Chuang RY, Weaver PL, Liu Z, Chang TH. 1997. Requirement of the DEAD-box protein ded1p for messenger RNA translation. Science 275:1468-1471.
18. Ding F, Hagan JP, Wang Z, Grabowski PJ. 1998. Biochemical properties of a novel U2AF65 protein isoform generated by alternative RNA splicing. Biochem Biophys Res Commun 224:675-683.
19. Dix I, Russell CS, O'Keefe RT, Newman AJ, Beggs JD. 1998. Protein-RNA interactions in the U5 snRNP of Saccharomyces cerevisiae. RNA 4:1239-1250.
20. Dyke MWV, Sirito M, Sawadogo M. 1992. Single-step purification of bacterially expressed polypeptides containing an oligo-histidine domain. Gene 111:99-104.
21. Faura M, Renau-Piqueras J, Bachs O, Bosser R. 1995. Differential distribution of heterogeneous nuclear ribonucleoproteins in rat tissues. Biochem Biophys Res Commun 17:554-560.
22. Feinberg AP, Vogelstein B. 1984. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem 137:266-267.
23. García-Blanco MA, Anderson GJ, Beggs J, Sharp PA. 1990. A mammalian protein of 220 kDa binds pre-mRNA in the spliceosome: A potential homologue of the yeast PRP9 protein. Proc Natl Acad Sci USA 87:3082-3086.
24. Gozani O, Patton JG, Reed R. 1994. A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction. EMBO J 13:3356-3367.
25. Guthrie C, Patterson B. 1988. Spliceosomal snRNA. Annu Rev Genet 22:387-419.
26. Hall SL, Padgett RA. 1996. Requirement of U12 snRNA for in vivo splicing of a minor class of eukaryotic nuclear pre-mRNA introns. Science 271:1716-1718.
27. Hanamura A, Caceres JF, Mayeda A, Franza BR, Krainer AR. 1998. Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors. RNA 4:430-444.
28. Hirose Y, Manley JL. 1998. RNA polymerase II is an essential mRNA polyadenylation factor. Nature 395:93-96.
29. Hodges PE, Jackson SP, Brown JD, Beggs JD. 1995. Extraordinary sequence conservation of the PRP8 splicing factor. Yeast 11:337-342.
30. Jackson SP, Lossky M, Beggs JD. 1988. Cloning of the RNA8 gene of Saccharomyces cerevisiae, detection of the RNA8 protein, and demonstration that it is essential for nuclear pre-mRNA splicing. Mol Cell Biol 8:1067-1075.
31. Jamieson DJ, Rahe B, Pringle J, Beggs JD. 1991. A suppressor of a yeast splicing mutation (prp8-1) encodes a putative ATP-dependent RNA helicase. Nature 349:715-717.
32. Jumaa H, Nielsen PJ. 1997. The splicing factor SRp20 modifies splicing of its own mRNA and ASF/SF2 antagonizes this regulation. EMBO J 16:5077-5085.
33. Kamma H, Portman DS, Dreyfuss G. 1995. Cell type-specific expression of hnRNP proteins. Exp Cell Res 221:187-196.
34. Kolossova I, Padgett RA. 1997. U11 snRNA interacts in vivo with the 5′ splice site of U12-dependent (AU-AC) pre-mRNA introns. RNA 3:227-233.
35. Konarska MM, Sharp PA. 1987. Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes. Cell 49:763-774.
36. Krämer A. 1996. The structure and function of proteins involved in mammalian pre-mRNA splicing. Ann Rev Biochem 65:367-409.
37. Kuhn AN, Li Z, Brow DA. 1999. Splicing factor Prp8 governs U4/U6 RNA unwinding during activation of the spliceosome. Mol Cell 3:65-75.
38. Lucke S, Klockner T, Palfi Z, Boshart M, Bindereif A. 1997. Trans mRNA splicing in trypanosomes: Cloning and analysis of a PRP8-homologous gene from Trypanosoma brucei provides evidence for a U5-analogous RNP. EMBO J 16:4433-4440.
39. MacMillan AM, Query CC, Allerson CR, Chen S, Verdine GL, Sharp PA. 1994. Dynamic association of proteins with the pre-mRNA branch region. Genes & Dev 8:3008-3020.
40. Manley JL, Tacke R. 1996. SR proteins and splicing control. Genes & Dev 10:1569-1579.
41. Moore MJ, Query CC, Sharp PA. 1993. Splicing of precursors to mRNA by the spliceosome. In: Gesteland R, Atkins J, eds. The RNA world. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press. pp 303-357.
42. Mortillaro MJ, Blencowe BJ, Wei X, Nakayasu H, Du L, Warren SL, Sharp PA, Berezney R. 1996. A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix. Proc Natl Acad Sci USA 93:8253-8257.
43. Nagy E, Maquat LE. 1998. A rule for termination-codon position within intron-containing genes: When nonsense affects RNA abundance. Trends Biochem Sci 23:198-199.
44. O'Keefe RT, Norman C, Newman AJ. 1996. The invariant U5 snRNA loop 1 sequence is dispensable for the first catalytic step of pre-mRNA splicing in yeast. Cell 86:679-689.
45. Orphanides G, Lagrange T, Reinberg D. 1996. The general transcription factors of RNA polymerase II. Genes & Dev 10:2657-2683.
46. Pinto AL, Steitz JA. 1989. The mammalian analogue of the yeast PRP8 splicing protein is present in the U4/5/6 small nuclear ribonucleoprotein particle and the spliceosome. Proc Natl Acad Sci USA 86:8742-8746.
47. Reyes JL, Gustafson EH, Luo HR, Moore MJ, Konarska MM. 1999. The C-terminal region of hPrp8 interacts with the conserved GU dinucleotide at the 5′ splice site. RNA 5:167-179.
48. Reyes JL, Kois P, Konforti BB, Konarska MM. 1996. The canonical GU dinucleotide at the 5′ splice site is recognized by p220 of the U5 snRNP within the spliceosome. RNA 2:213-225.
49. Ruby SW, Abelson J. 1991. Pre-mRNA splicing in yeast. Trends Genet 7:79-85.
50. Sharp PA, Burge CB. 1997. Classification of introns: U2-type or U12-type. Cell 91:875-879.
51. Shea JE, Toyn JH, Johnston LH. 1994. The budding yeast U5 snRNP Prp8 is a highly conserved protein which links RNA splicing with cell cycle progression. Nucleic Acids Res 22:5555-5564.
52. Sontheimer EJ, Steitz JA. 1992. Three novel functional variants of human U5 small nuclear RNA. Mol Cell Biol 12:734-746.
53. Strauss EJ, Guthrie C. 1991. A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family. Genes & Dev 5:629-641.
54. Tarn WY, Steitz JA. 1996a. Highly diverged U4 and U6 small nuclear RNAs required for splicing rare AT-AC introns. Science 273:1824-1832.
55. Tarn WY, Steitz JA. 1996b. A novel spliceosome containing U11, U12, and U5 snRNPs excises a minor class (AT-AC) intron in vitro. Cell 84:801-811.
56. Tarn WY, Steitz JA. 1997. Pre-mRNA splicing: The discovery of a new spliceosome doubles the challenge. Trends Biochem Sci 22:132-137.
57. Teigelkamp S, Mundt CS, Achsel T, Will CL, Luhrmann R. 1997. The human U5 snRNP-specific 100-kD protein is an RS domain-containing, putative RNA helicase with significant homology to the yeast splicing factor Prp28p. RNA 3:1313-1326.
58. Teigelkamp S, Newman AJ, Beggs JD. 1995a. Extensive interactions of PRP8 protein with the 5′ and 3′ splice sites during splicing suggest a role in stabilization of exon alignment by U5 snRNA. EMBO J 14:101-111.
59. Teigelkamp S, Whittaker E, Beggs JD. 1995b. Interaction of the yeast splicing factor PRP8 with substrate RNA during both steps of splicing. Nucleic Acids Research 23:320-326.
60. Umen JG, Guthrie C. 1995a. A novel role for a U5 snRNP protein in 3′ splice site selection. Genes & Dev 9:855-868.
61. Umen JG, Guthrie C. 1995b. Prp16p, Slu7p, and Prp8p interact with the 3′ splice site in two distinct stages during the second catalytic step of pre-mRNA splicing. RNA 1:584-597.
62. Umen JG, Guthrie C. 1996. Mutagenesis of the yeast gene PRP8 reveals domains governing the specificity and fidelity of 3′ splice site selection. Genetics 143:723-739.
63. Vijayraghavan U, Company M, Abelson J. 1989. Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes & Dev 3:1206-1216.
64. Whittaker E, Beggs JD. 1991. The yeast PRP8 protein interacts directly with pre-mRNA. Nucleic Acids Res 19:5483-5489.
65. Whittaker E, Lossky M, Beggs JD. 1990. Affinity purification of spliceosomes reveals that the precursor RNA processing protein PRP8, a protein in the U5 small nuclear ribonucleoprotein particle, is a component of yeast spliceosomes. Proc Natl Acad Sci USA 87:2216-2219.
66. Will CL, Lührmann R. 1997. Protein functions in pre-mRNA splicing. Curr Opin Cell Biol 9:320-328.
67. Wu Q, Krainer AR. 1996. U1-mediated exon definition interactions between AT-AC and GT-AG introns. Science 274:1005-1008.
68. Wyatt JR, Sontheimer EJ, Steitz JA. 1992. Site-specific crosslinking of mammalian U5 snRNP to the 5′ splice site prior to the first step of premessenger RNA splicing. Genes & Dev 6:2542-2553.
69. Xu D, Field DJ, Tang SJ, Moris A, Bobechko BP, Friesen JD. 1998. Synthetic lethality of yeast sit mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing. Mol Cell Biol 18:2055-2066.