Infectious cDNA clone of the epidemic West Nile virus from New York City

Journal of Virology

Volumn 76, Issue 12, 2002, Pages 5847-5856

  Shi, Pei Yong ^a   Tilgner, Mark ^a   Lo, Michael K ^a   Kent, Kim A ^a   Bernard, Kristen A ^a  

^a WADSWORTH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL STRAIN BHK; CONTROLLED STUDY; EPIDEMIC; ESCHERICHIA COLI; FEMALE; GENE AMPLIFICATION; GENE CONSTRUCT; GENE DELETION; GENETIC ENGINEERING; GENETIC LINKAGE; GENETIC MARKER; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROGENY; RNA TRANSCRIPTION; UNITED STATES; VIRUS GENOME; VIRUS STRAIN; VIRUS VIRULENCE; WEST NILE FLAVIVIRUS;

ANIMALS; CERCOPITHECUS AETHIOPS; CLONING, MOLECULAR; DISEASE OUTBREAKS; DNA, COMPLEMENTARY; DNA, VIRAL; HUMANS; MICE; NEW YORK CITY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; VERO CELLS; WEST NILE FEVER; WEST NILE VIRUS;

EID: 0036107695     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.76.12.5847-5856.2002     Document Type: Article

References (70)

1. De novo synthesis ot RNA by the dengue virus RNA-dependent RNA polymerase exhibits temperature dependence at the initiation but not elongation phase
2. Isolation of West Nile virus from mosquitoes, crows, and a Cooper's hawk in Connecticut
3. West Nile virus activity in the United States, 2001
4. Extensive nucleotide changes and deletions within the envelope glycoprotein gene of Euro-African West Nile viruses
5. BHK cell proteins that bind to the 3′ stem-loop structure of the West Nile virus genome RNA
6. Translation elongation factor-1 alpha interacts with the 3′ stem-loop region of West Nile virus genomic RNA
7. Sequence and secondary structure analysis of the 5′-terminal region of flavivirus genome RNA
8. The 3′-nucleotides of flavivirus genomic RNA form a conserved secondary, structure
9. Flaviviruses
10. Attenuation markers of a candidate dengue type 2 vaccine virus, strain 16681 (PDK-53), are defined by mutations in the 5′ noncoding region and nonstructural proteins 1 and 3
11. Infectious cDNA clones of Langat tick-borne flavivirus that differ from their parent in peripheral neurovirulence
12. Nucleotide changes responsible for loss of neuroinvasiveness in Japanese encephalitis virus neutralization-resistant mutants
13. Guidelines for surveillance, prevention and control of West Nile virus infection-United States
14. Weekly update: West Nile virus activity - United States, November 14-20, 2001
15. Flavivirus genome organization, expression, and replication
16. West Nile virus envelope proteins: Nucleotide sequence analysis of strains differing in mouse neuroinvasiveness
17. RNA-protein interactions: Involvement of NS3, NS5, and 3′ noncoding regions of Japanese encephalitis virus genomic RNA
18. Partial genetic characterization of West Nile virus strains, New York State, 2000
19. Infectious transcripts of tick-borne encephalitis virus, generated in days by RT-PCR
20. Loss of dimerisation of the nonstructural protein NS1 of Kunjin virus delays viral replication and reduces virulence in mice, but still allows secretion of NS1
21. Mutations in the envelope protein of Japanese encephalitis virus affect entry into cultured cells and virulence in mice
22. Differences in fusogenicity and mouse neurovirulence of Japanese encephalitis viruses
23. Mutational analysis of a neutralization epitope on the dengue type 2 virus (DEN2) envelope protein: Monoclonal antibody resistant DEN2/DEN4 chimeras exhibit reduced mouse neurovirulence
24. Amino acid substitution(s) in the stem-anchor region of Langat virus envelope protein attenuates mouse neurovirulence
25. A single amino acid substitution in envelope protein E of tick-borne encephalitis virus leads to attenuation in the mouse model
26. Characterization of monoclonal antibody-escape mutants of tick-borne encephalitis virus with reduced neuroinvasiveness in mice
27. Characterization of infectious Murray Valley encephalitis virus derived from a stably cloned genome-length cDNA
28. Genetic analysis of West Nile New York 1999 encephalitis virus
29. Single amino acid codon changes detected in louping ill virus antibody-resistant mutants with reduced neurovirulence
30. Discovery and molecular characterization of West Nile virus NY 1999
31. Synthesis and characterization of an infectious dengue virus type-2 RNA genome (New Guinea C strain)
32. Essential role of cyclization sequences in flavivirus RNA replication
33. Completion of Kunjin virus RNA sequence and recovery of an infectious RNA transcribed from stably cloned full-length cDNA
34. Infectious RNA transcribed from stably cloned full-length cDNA of dengue type 4 virus
35. Complete genome sequence and phylogenetic analysis of West Nile virus strains isolated from the United States, Europe and the Middle East
36. Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States
37. Substitutions at the putative receptor-binding site of an encephalitic flavivirus alter virulence and host cell tropism and reveal a role for glycosaminoglycans in entry
38. The 3′ stem loop of the West Nile virus genomic RNA can suppress translation of chimeric mRNAs
39. Flaviviridae: The viruses and their replication
40. Infectious cDNA clones of tick-borne encephalitis virus European subtype prototypic strain Neudoerfl and high virulence strain Hypr
41. Spontaneous and engineered deletions in the 3′ noncoding region of tick-borne encephalitis virus: Construction of highly attenuated mutants of a flavivirus
42. Widespread West Nile Virus activity, Eastern United States, 2000
43. The molecular basis of virulence of the encephalitogenic flaviviruses
44. A comparison of the spread of Murray Valley encephalitis viruses of high or low neuroinvasiveness in the tissues of Swiss mice after peripheral inoculation
45. Dengue type 4 virus mutants containing deletions in the 3′ noncoding region of the RNA genome: Analysis of growth restriction in cell culture and altered viremia pattern and immunogenicity in rhesus monkeys
46. Mutagenesis of the N-linked glycosylation sites of the yellow fever virus NS1 protein: Effects on virus replication and mouse neurovirulence
47. Attenuation of Japanese encephalitis virus by selection of its mouse brain membrane receptor preparation escape variants
48. Chimeric tick-borne encephalitis and dengue type 4 viruses: Effects of mutations on neurovirulence in mice
49. Infectious RNA transcripts from full-length dengue virus type 2 cDNA clones made in yeast
50. Secondary structure of the 3′ untranslated region of flaviviruses: Similarities and differences
51. Growth restriction of dengue virus type 2 by site-specific mutagenesis of virus-encoded glycoproteins
52. Secondary structure of the 3′-noncoding region of flavivirus genomes: Comparative analysis of base pairing probabilities
53. Effect of temperature on the transmission of western equine encephalomyelitis and St. Louis encephalitis viruses by Culex tarsalis (Diptera: Culicidae)
54. Transcription of infectious yellow fever RNA from full-length cDNA templates produced by in vitro ligation
55. Nucleotide sequence of yellow fever virus: Implications for flavivirus gene expression and evolution
56. Functional cDNA clones of the Flaviviridae: Strategies and applications
57. A single nucleotide change in the E protein gene of dengue virus 2 Mexican strain affects neurovirulence in mice
58. Entomologic and avian investigations of an epidemic of West Nile fever in Romania in 1996, with serologic and molecular characterization of a virus isolate from mosquitoes
59. Evidence for the existence of a pseudoknot structure at the 3′ terminus of the flavivirus genomic RNA
60. Construction of infectious dengue 2 virus cDNA clones using high copy number plasmid
61. Infectious Japanese encephalitis virus RNA can be synthesized from in vitro-ligated cDNA templates
62. West Nile encephalitis epidemic in southeastern Romania
63. Cytopathic effect of rubella virus in BHK-21 cells and growth to high titers in suspension culture
64. Japanese encephalitis virus antigenic variants with characteristic differences in neutralization resistance and mouse virulence
65. Mutation in NS5 protein attenuates mouse neurovirulence of yellow fever 17D vaccine virus
66. A new strategy in design of +RNA virus infectious clones enabling their stable propagation in E. coli
67. An infectious clone of the West Nile flavivirus
68. A novel in vitro replication system for dengue virus. Initiation of RNA synthesis at the 3′-end of exogenous viral RNA templates requires 5′-and 3′-terminal complementary sequence motifs of the viral RNA
69. Identification of specific nucleotide sequences within the conserved 3′-SL in the dengue type 2 virus genome required for replication