Loss of dimerisation of the nonstructural protein NS1 of Kunjin virus delays vital replication and reduces virulence in mice, but still allows secretion of NS1

Virology

Volumn 264, Issue 1, 1999, Pages 66-75

  Hall, Roy A ^a   Khromykh, Alexander A ^b   MacKenzie, Jason M ^b   Scherret, Jacqueline H ^a   Khromykh, Tatiana I ^a   MacKenzie, John S ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b ROYAL CHILDREN'S HOSPITAL   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

LEUCINE; PROLINE; PROTEIN NS1; UNCLASSIFIED DRUG; VIRUS RNA;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DIMERIZATION; FLAVIVIRUS; KUNJIN VIRUS; MOUSE; NONHUMAN; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; VERO CELL; VIRUS GENE; VIRUS REPLICATION; VIRUS VIRULENCE;

ANIMALIA; FLAVIVIRUS; KUNJIN VIRUS; RNA VIRUSES;

EID: 0033544434     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1006/viro.1999.9956     Document Type: Article

References (39)

1. Adams S. C., Broom A. K., Sammels L. M., Hartnett A. C., Howard M. J., Coelen R. J., Mackenzie J. S., Hall R. A. Glycosylation and antigenic variation among Kunjin virus isolates. Virology. 206:1995;49-56.
2. Blitvich B. J., Coelen R. J., Mackenzie J. S., Howard M. J., Hall R. A. A novel complex formed between the flavivirus E and NS1 proteins: Characterisation of its structure and function. Arch. Virol. 140:1995;145-156.
3. Blok J., McWilliam S. M., Butler H. C., Gibbs A. J., Weiller G., Herring B. L., Hemsley A. C., Aaskov J. G., Yoksan S., Bhamarapravati N. Comparison of a dengue-2 virus and its candidate vaccine derivative: Sequence relationships with the flaviviruses and other viruses. Virology. 187:1992;573-590.
4. Branden C., Tooze J. Introduction to Protein Structure. 1991.
5. Castle E., Leidner U., Nowak T., Wengler G., Wengler G. Primary structure of the West Nile flavivirus genome region coding for all nonstructural proteins. Virology. 149:1986;10-26.
6. Coia G., Parker M. D., Speight G., Byrne M. E., Westaway E. G. Nucleotide and complete amino acid sequence of Kunjin virus: Definitive gene order and characteristics of the virus-specified proteins. J. Gen. Virol. 69:1988;1-21.
7. Crooks A. J., Lee J. M., Easterbrook L. M., Timofeev A. V., Stephenson J. R. The NS1 protein of tick-borne encephalitis virus forms multimeric species upon secretion from the host cell. J. Gen. Virol. 75:1994;3453-3460.
8. Dalgarno L., Trent D. W., Strauss J. H., Rice C. M. Partial nucleotide sequence of the Murray Valley encephalitis virus genome: Comparison of the encoded polypeptides with Yellow Fever virus structural and nonstructural proteins. J. Mol. Biol. 187:1986;309-323.
9. Fan W., Mason P. W. Membrane association and secretion of the Japanese encephalitis virus NS1 protein from cells expressing NS1 cDNA. Virology. 177:1990;470-476.
10. Flamand M., Deubel V., Girard M. Expression and secretion of Japanese Encephalitis virus nonstructural protein NS1 by insect cells using a recombinant baculovirus. Virology. 191:1992;826-836.
11. Flamand M., Megret F., Mathieu M., Lepault J., Rey F. A., Deubel V. Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion. J. Virol. 73:1999;6104-6110.
12. Fu J., Tan B-H., Yap E-H., Chan Y-C., Tan Y. H. Full length cDNA sequence of dengue type 1 virus (Singapore strain S275/90). Virology. 188:1992;953-958.
13. Hall R. A., Kay B. H., Burgess G. W., Clancy P., Fanning I. D. Epitope analysis of the envelope and non-structural glycoproteins of Murray Valley encephalitis virus. J. Gen. Virol. 71:1990;2923-2930.
14. Khromykh A. A., Westaway E. G. Completion of Kunjin virus RNA sequence and recovery of an infectious RNA transcribed from stably cloned full-length cDNA. J. Virol. 68:1994;4580-4588.
15. Khromykh A. A., Westaway E. G. Subgenomic replicons of the flavivirus Kunjin: Construction and applications. J. Virol. 71:1997;1497-1505.
16. Khromykh A. A., Kenney M. T., Westaway E. G. trans-complementation of flavivirus RNA polymerase gene NS5 by using Kunjin virus replicon-expressing BHK cells. J. Virol. 72:1998;7270-7279.
17. Lindenbach B. D., Rice C. M. trans-Complementation of Yellow Fever virus NS1 reveals a role in early RNA replication. J. Virol. 71:1997;9608-9617.
18. Lindenbach B. D., Rice C. M. Genetic interaction of flavivirus nonstructural proteins NS1 and NS4A as a determinant of replicase function. J. Virol. 73:1999;4611-4621.
19. Mackenzie J. M., Jones M. K., Young P. R. Immunolocalisation of the dengue virus nonstructural glycoprotein NS1 suggests a role in viral RNA replication. Virology. 220:1996;232-240.
20. Mackow E., Makino Y., Zhao B. T., Zhang Y. M., Markoff L., Buckler-White A., Guiler M., Chanock R., Lai C. J. The nucleotide sequence of dengue type 4 virus: Analysis of genes coding for nonstructural proteins. Virology. 159:1987;217-228.
21. Mandl C. W., Heinz F. X., Stockl E., Kunz C. Genome sequence of tick-borne encephalitis virus (Western subtype) and comparative analysis of nonstructural proteins with other flaviviruses. Virology. 173:1989;291-301.
22. Mason P. W. Maturation of Japanese Encephalitis virus glycoproteins produced by infected mammalian and mosquito cells. Virology. 169:1989;354-364.
23. Muylaert I. R., Galler R., Rice C. M. Genetic analysis of the yellow fever virus NS1 protein: Identification of a temperature-sensitive mutation which blocks RNA accumulation. J. Virol. 71:1997;291-298.
24. Osatomi K., Sumiyoshi H. Complete nucleotide sequence of dengue type 3 virus genome RNA. Virology. 176:1990;643-647.
25. Poidinger M., Hall R. A., Mackenzie J. S. Molecular characterisation of the Japanese encephalitis serocomplex of the flavivirus genus. Virology. 218:1996;417-421.
26. Pryor M. J., Wright P. J. The effects of site-directed mutagenesis on the dimerization and secretion of the NS1 protein specified by Dengue virus. Virology. 194:1993;769-780.
27. Pryor M. J., Wright P. J. Glycosylation mutants of Dengue virus NS1 protein. J. Gen. Virol. 75:1994;1183-1187.
28. Pryor M. J., Gualano R. C., Lin B., Davidson A. D., Wright P. J. Growth restriction of dengue virus type 2 by site-specific mutagenesis of virus-encoded glycoproteins. J. Gen. Virol. 79:1998;2631-2639.
29. Reed L. J., Meunch H. A simple method for estimating fifty percent endpoints. Am. J. Hyg. 27:1938;493-497.
30. Rice C. M. Flaviviridae: The viruses and their replication. Fields B. N., Knipe D. M., Howley P. M. Fields Virology. 1996;931-959 Lippincott-Raven Publishers, Philadelphia.
31. Rice C. M., Lenches E. M., Eddy S. R., Shin S. J., Sheets R. L., Strauss J. H. Nucleotide sequence of yellow fever virus: Implications for flavivirus gene expression and evolution. Science. 229:1985;726-733.
32. Schlesinger J. J., Brandriss M. W., Cropp C. B., Monath T. P. Protection against Yellow Fever in monkeys by immunization with Yellow Fever virus nonstructural protein NS1. J. Virol. 60:1986;1153-1155.
33. Schlesinger J. J., Brandriss M. W., Putnak J. R., Walsh E. E. Cell surface expression of yellow fever virus non-structural glycoprotein NS1: Consequences of interaction with antibody. J. Gen. Virol. 71:1990;593-599.
34. Sumiyoshi H., Mori C., Fuke I., Morita K., Kuhara S., Kondou J., Kikuchi Y., Nagamatu H., Igarashi A. Complete nucleotide sequence of the Japanese encephalitis virus genome RNA. Virology. 161:1987;497-510.
35. Timofeev A. V., Ozherelkov S. V., Pronin A. V., Deeva A. V., Karganova G. G., Elbert L. B., Stephenson J. R. Immunological basis for protection in a murine model of tick-borne encephalitis by a recombinant adenovirus carrying the gene encoding the NS1 non-structural protein. J. Gen. Virol. 79:1998;689-695.
36. Trent D. W., Kinney R. M., Johnson B. J. B., Vorndam A. V., Grant J. A., Deubel V., Rice C. M., Hahn C. Partial nucleotide sequence of St. Louis encephalitis RNA: Structural proteins, NS1, ns2a and ns2b. Virology. 156:1987;293-304.
37. Westaway E. G., Mackenzie J. M., Kenney M. T., Jones M. K., Khromykh A. A. Ultrastructure of Kunjin virus-infected cells: Colocalization of NS1 and NS3 with double-stranded RNA, and of NS2B with NS3, in virus-induced membrane structures. J. Virol. 71:1997;6650-6661.
38. Winkler G., Randolph V. B., Cleaves G. R., Ryan T. E., Stollar V. Evidence that the mature form of the flavivirus nonstructural protein NS1 is a dimer. Virology. 162:1988;187-196.
39. Winkler G., Maxwell S. E., Ruemmler C., Stollar V. Newly synthesized Dengue-2 virus nonstructural protein NS1 is a soluble protein but becomes partially hydrophobic and membrane-associated after dimerization. Virology. 171:1989;302-305.