Specificity modulation of barley α-amylase through biased random mutagenesis involving a conserved tripeptide in β → α loop 7 of the catalytic (β/α)8-barrel domain

Biochemistry

Volumn 40, Issue 43, 2001, Pages 12844-12854

  Gottschalk, T E ^a,b   Tull, D ^a,c   Aghajari, N ^d   Haser, R ^d   Svensson, B ^a  

^a CARLSBERG LABORATORY   (Denmark)

^b Pharmexa AS   (Denmark)

^c UNIVERSITY OF MELBOURNE   (Australia)

^d UNIVERSITÉ LYON 1   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CLEAVAGE PATTERN;

CATALYSIS; ENZYMES; MODULATION; STABILIZATION; STARCH; YEAST;

MUTAGENESIS;

2 CHLORO 4 NITROPHENYL BETA DEXTRO MALTOHEPTAOSIDE; 4 NITROPHENYL ALPHA DEXTRO MALTOTRIOSIDE; AMYLASE; AMYLOSE; ASPARTIC ACID; GLUTATHIONE; GLYCOSIDASE; HISTIDINE; MALTOSE; OLIGOSACCHARIDE; STARCH; TRIPEPTIDE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CATALYSIS; CONTROLLED STUDY; ENZYME BINDING; ENZYME INACTIVATION; ENZYME SPECIFICITY; ENZYME STABILITY; GENETIC ENGINEERING; LIGAND BINDING; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FOLDING; SEQUENCE ANALYSIS;

ALPHA-AMYLASE; AMINO ACID SEQUENCE; CATALYTIC DOMAIN; CONSERVED SEQUENCE; HORDEUM; KINETICS; LIGANDS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTATION; PEPTIDES; PLASMIDS; POLYMERASE CHAIN REACTION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY, AMINO ACID;

HORDEUM VULGARE SUBSP. VULGARE;

EID: 0035980244     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0108608     Document Type: Article

References (75)