Specific inhibition of barley α-amylase 2 by barley α- amylase/subtilisin inhibitor depends on charge interactions and can be conferred to isozyme 1 by mutation

European Journal of Biochemistry

Volumn 267, Issue 4, 2000, Pages 1019-1029

  Rodenburg, Kees W ^a,e   Vallée, Francois ^b,f   Juge, Nathalie ^a,c   Aghajari, Nushin ^d   Guo, Xiao jun ^c   Haser, Richard ^d   Svensson, Birte ^a  

^a CARLSBERG LABORATORY   (Denmark)

^b CNRS and Aix Marseille University   (France)

^c AIX MARSEILLE UNIVERSITY   (France)

^d CNRS   (France)

^e Department of Biology   (Netherlands)

^f HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

Barley amylase subtilisin inhibitor; Charge screening; Enzyme engineering of inhibitor recognition; Protein protein interactions; Site directed mutagenesis

Indexed keywords

AMYLASE; AMYLASE INHIBITOR; SUBTILISIN;

AMINO ACID SEQUENCE; ARTICLE; BARLEY; ENZYME INHIBITOR INTERACTION; MOLECULAR INTERACTION; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SITE DIRECTED MUTAGENESIS;

ALPHA-AMYLASE; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BINDING SITES; CALCIUM; ELECTROSTATICS; ENZYME STABILITY; HORDEUM; HYDROGEN BONDING; HYDROGEN-ION CONCENTRATION; ISOELECTRIC POINT; ISOENZYMES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PLANT PROTEINS; RECOMBINANT FUSION PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; THERMODYNAMICS; TRYPSIN INHIBITOR, KUNITZ SOYBEAN;

HORDEUM VULGARE SUBSP. VULGARE;

EID: 0033969448     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01094.x     Document Type: Article

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