NTF2 monomer-dimer equilibrium

Journal of Molecular Biology

Volumn 314, Issue 3, 2001, Pages 465-477

  Chaillan Huntington, Catherine ^a   Butler, P Jonathan G ^a   Huntington, James A ^b   Akin, Debra ^c   Feldherr, Carl ^c   Stewart, Murray ^a  

^a MEDICAL RESEARCH COUNCIL   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

Author keywords

Crystallography; Dimerization; Nuclear trafficking; Protein structure

Indexed keywords

CELL PROTEIN; MESSENGER RNA; NUCLEAR TRANSPORT FACTOR 2; NUCLEOPORIN; RAN PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; BINDING SITE; CELL TRANSPORT; DIMERIZATION; DISSOCIATION CONSTANT; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN SECRETION; PROTEIN STRUCTURE; THERMOSTABILITY; XENOPUS;

EID: 0035976709     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.5136     Document Type: Article

References (50)

1. Transport between the cell nucleus and the cytoplasm
2. Transport of proteins and RNAs in and out of the nucleus
3. The molecular mechanism of transport of macromolecules through nuclear pore complexes
4. Gatekeepers of the nucleus
5. Pores for thought: Nuclear pore complex proteins
6. The yeast nuclear pore complex: Composition, architecture and transport mechanism
7. The nuclear pore complex: A protein machine bridging the nucleus and cytoplasm
8. From nucleoporins to nuclear pore complexes
9. The mechanism of ran import into the nucleus by nuclear transport factor 2
10. Running on ran: Nuclear transport and the mitotic spindle
11. Two-way trafficking with Ran
12. Transport into and out of the cell nucleus
13. Structural basis for the interaction between FxFG nucleoporin repeats and importin-β in nuclear trafficking
14. Interaction between NTF2 and xFxFG-containing nucleoporins is required to mediate nuclear import of RanGDP
15. Mapping interactions between nuclear transport factors in living cells reveals pathways through the nuclear pore complex
16. NTF2 mediates nuclear import of Ran
17. Nuclear import of Ran is mediated by the transport factor NTF2
18. Insights into the molecular mechanism of nuclear trafficking using nuclear transport factor 2 (NTF2)
19. Nuclear transport factor p10/NTF2 functions as a Ran-GDP dissociation inhibitor (Ran-GDI)
20. Dissecting the interactions between NTF2, RanGDP and the nucleoporin xFxFG repeats
21. The NTF2 gene encodes an essential, highly conserved protein that functions in nuclear transport in vivo
22. High levels of the GTPase Ran/TC4 relieve the requirement for nuclear protein transport factor 2
23. Selective disruption of nuclear import by a functional mutant nuclear carrier protein
24. Prediction of structural domains of TAP reveals details of its interaction with p15 and nucleoporins
25. The structure of the mRNA export factor TAP reveals a cis arrangement of a non-canonical RNP domain and an LRR domain
26. The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human
27. Identification of an NTF2-related factor that binds Ran-GTP and regulates nuclear protein export
28. NXT1 is necessary for the terminal step of Crm1-mediated nuclear export
29. The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates
30. Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG and FG repeat nucleoporins is essential for nuclear mRNA export
31. The 1.6 Å resolution crystal structure of nuclear transport factor 2 (NTF2)
32. Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran
33. Macromolecular crowding: An important but neglected aspect of the intracellular environment
34. Separate binding sites on nuclear transport factor 2 (NTF2) for GDP-Ran and the phenylalanine-rich repeat regions of nucleoporins p62 and Nsp1p
35. Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP Ran
36. The nuclear import factor p10 regulates the functional size of the nuclear pore complex during oogenesis
37. Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore protein p62
38. Purification of a Ran-interacting protein that is required for protein import into the nucleus
39. Interaction between the small GTPase Ran/Gsp1p and Ntf2p is required for nuclear transport
40. Rapid attainment of sedimentation equilibrium
41. Computer-aided interpretation of analytical sedimentation data for proteins
42. Comments on the analysis of sedimentation equilibrium experiments
43. The CCP4 suite: Programs for protein crystallography
44. Crystallography and NMR system: A new software suite for macromolecular structure determination
45. Free R value: A novel statistical quality for assessing the accuracy of crystal structures
46. The solvent content of protein crystals
47. Improved methods for building protein models in electron density maps and for locating errors in these models
48. An extensively modified version of MolScript that includes greatly enhanced colouring capabilities
49. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
50. Translocation of RNA-coated gold particles through the nuclear pores of oocytes