Selective disruption of nuclear import by a functional mutant nuclear transport carrier

Journal of Cell Biology

Volumn 151, Issue 2, 2000, Pages 321-331

  Lane, Cynthia M ^a   Cushman, Ian ^a   Moore, Mary Shannon ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

Karyopherin; NTF2; Nuclear pore complex; Nuclear transport; P10

Indexed keywords

GUANINE NUCLEOTIDE EXCHANGE FACTOR; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; KARYOPHERIN; NUCLEOPORIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; BINDING SITE; CELL NUCLEUS; HELA CELL; HUMAN; HUMAN CELL; MEMBRANE PERMEABILITY; MEMBRANE TRANSPORT; PRIORITY JOURNAL;

ACTIVE TRANSPORT, CELL NUCLEUS; BETA KARYOPHERINS; BINDING SITES; CARRIER PROTEINS; GUANOSINE DIPHOSPHATE; NUCLEAR LOCALIZATION SIGNALS; NUCLEAR PORE; NUCLEAR PROTEINS; NUCLEOCYTOPLASMIC TRANSPORT PROTEINS; PEPTIDE FRAGMENTS; POINT MUTATION; PROTEIN BINDING; RAN GTP-BINDING PROTEIN; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 0034675931     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.151.2.321     Document Type: Article

References (58)

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3. Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide
4. Identification of an NTF2-related factor that binds Ran-GTP and regulates nuclear protein export
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6. The 1.6 angstroms resolution crystal structure of nuclear transport factor 2 (NTF2)
7. Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP-Ran
8. Separate binding sites on nuclear transport factor 2 (NTF2) for GDP-Ran and the phenylalanine-rich repeat regions of nucleoporins p62 and Nsp1p
9. The NTF2 gene encodes an essential, highly conserved protein that functions in nuclear transport in vivo
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14. Disassembly of RanGTP-karyophefin β complex, an intermediate in nuclear protein import
15. The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88
16. A novel class of RanGTP binding proteins
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18. Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins
19. The GLFG repetitive region of the nucleoporin Nup116p interacts with Kap95p, an essential yeast nuclear import factor
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21. The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human
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29. The two steps of nuclear import, targeting to the nuclear envelope and translocation through the nuclear pore, require different cytosolic factors
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31. Transport of proteins and RNAs in and out of the nucleus
32. Nup153 is an M9-containing mobile nucleoporin with a novel Ran-binding domain
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36. Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62
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39. A distinct and parallel pathway for the nuclear import of an mRNA-binding protein
40. Molecular interactions between the importin α/β heterodimer and proteins involved in vertebrate nuclear protein import
41. Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins
42. The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex
43. Ran/TC4: A small nuclear GTP-binding protein that regulates DNA synthesis
44. Protein import into nuclei: Association and dissociation reactions involving transport substrate, transport factors, and nucleoporins
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48. The nuclear pore complex: A protein machine bridging the nucleus and cytoplasm
49. Ran-dependent signal-mediated nuclear import does not require GTP hydrolysis by Ran
50. Separate nuclear import pathways converge on the nucleoporin nup153 and can be dissected with dominant-negative inhibitors
51. Nuclear import of Ran is mediated by the transport factor NTF2
52. Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran
53. Exogenously injected nuclear import factor p10/NTF2 inhibits signal-mediated nuclear import and export of proteins in living cells
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56. A new family of yeast nuclear pore complex proteins
57. Interaction between the small GTPase Ran/Gsp1p and Ntf2p is required for nuclear transport
58. Two distinct classes of Ran-binding sites on the nucleoporin Nup-358