Identification of a Δ4 Fatty Acid Desaturase from Thraustochytrium sp. Involved in the Biosynthesis of Docosahexanoic Acid by Heterologous Expression in Saccharomyces cerevisiae and Brassica juncea

Journal of Biological Chemistry

Volumn 276, Issue 34, 2001, Pages 31561-31566

  Qiu, Xiao ^a,b   Hong, Haiping ^a,b   MacKenzie, Samuel L ^b  

^a Bioriginal Food and Science Corporation   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; ENZYMES; ORGANIC ACIDS; SYNTHESIS (CHEMICAL); TISSUE;

VEGETATIVE TISSUES;

BIOCHEMISTRY;

ACYL COENZYME A DESATURASE; COMPLEMENTARY DNA; DOCOSAHEXAENOIC ACID; DELTA 4 FATTY ACID DESATURASE; DELTA 5 FATTY ACID DESATURASE; DELTA-4 FATTY ACID DESATURASE; DELTA-5 FATTY ACID DESATURASE;

ALGA; ARTICLE; BRASSICA; BRASSICA JUNCEA; CHEMICAL BOND; CROP PRODUCTION; ENZYME ANALYSIS; FATTY ACID DESATURATION; FATTY ACID SYNTHESIS; GAS CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; PHYLOGENY; PRIORITY JOURNAL; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; THRAUSTOCHYTRIUM; AMINO ACID SEQUENCE; CHEMISTRY; ENZYMOLOGY; EUKARYOTIC CELL; GENETICS; MASS FRAGMENTOGRAPHY; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS;

ALGAE; BRASSICA; BRASSICA JUNCEA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; THRAUSTOCHYTRIUM; THRAUSTOCHYTRIUM SP.;

AMINO ACID SEQUENCE; BRASSICA; CLONING, MOLECULAR; DOCOSAHEXAENOIC ACIDS; EUKARYOTIC CELLS; FATTY ACID DESATURASES; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; SACCHAROMYCES CEREVISIAE;

EID: 0035943661     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M102971200     Document Type: Article

References (46)

1. Crawford, M. A., Costeloe, K., Ghebremeskel, K., Phylactos, A., Skirvin, L. & Stacey, F. (1997) Am. J. Clin. Nutr. 66, 1032S-1041S
2. Giusto, N. M., Pasquare, S. J., Salvador, P. I., Roque, M. E. & Ilincheta de Boschero, M. G. (2000) Prog. Lipid Res. 39, 315-391
3. Martinetz, M. (1992) J. Pediatr. 120, S129-S138
4. Horrocks, L. A. & Yeo, Y. K. (1999) Pharmacol. Res. 40, 211-215
5. Spector, A. A. (1999) Lipids 34, S1-S3
6. Horrobin, D. F. (1992) Prog. Lipid Res. 31, 163-194
7. Gurr, M., I., (1992) Lipid Biotech. 4, 141-143
8. Sprecher, H., Luthria, D. L., Mohammed B. S. & Baykousheva, S. P. (1995) J. Lipid Res. 36, 2471-2477
9. Sprecher, H., Chen, Q. & Yin F. Q. (1999) Lipids, S153-S156
10. Sprecher, H. (2000) Biochim. Biophys. Acta 1486, 219-231
11. Voss, A., Reinhart, M., Sankarappa, S. & Sprecher, H. (1991) J. Biol. Chem. 266, 19995-20000
12. Weete, J. D., Kim, H., Gandhi, S. R., Wang, Y. & Dute R. (1997) Lipids 32, 839-845
13. Lewis, T. E., Nichols, P. D. & McMeekin, T. A. (1999) Mar. Biotechnol. 1, 580-587
14. Napier, J. A., Sayanova, O., Sperling, P. & Heinz E. (1999) Trends Plant Sci. 4, 2-4
15. Qiu, X. & Erickson, L. (1994) Plant Mol. Biol. Repr. 12, 209-214
16. Qiu, X., Reed, D. R., Hong, H. P., Mackenzie, S. L & Covello, P. S. (2001) Plant Physiol. 125, 847-855
17. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, pp. 8.46-8.52, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
18. Radke, S. E., Turner, J. C. & Facciotti, D. (1992) Plant Cell Rep. 11, 499-505
19. Cho, H. P., Nakamura, M. T. & Clarke, S. D. (1999) J. Biol. Chem. 274, 471-477
20. Cho, H. P., Nakamura, M. & Clarke, S. D. (1999) J. Biol. Chem. 274, 37335-37339
21. Watts, J. L. & Browse, J. (1999) Arch. Biochem. Biophys. 362, 175-182
22. Huang, Y., Chaudhary, S., Thurmond, J. M., Bobik, E. G., Yuan, L., Chan, G., Kirchner, S. J., Mukerji, P. & Knutzon, D. S. (1999) Lipids 34, 649-659
23. Knutzon, S. D., Thurmond, J. M., Huang, Y. S., Chaudhary, S., Bobik, E. G., Jr., Chan, G., Kirchner, S. J. & Mukerji, P. (1998) J. Biol. Chem. 273, 29360-29366
24. Michaelson, L. V., Lazarus, C. M., Griffiths, G. & Napier, J. A. (1998) J. Biol. Chem. 273, 19055-19059
25. Saito, T., Morio, T. & Ochiai, H. (2000) Eur. J. Biochem. 267, 1813-1818
26. Sperling, P., Lee, M., Girke, T., Zaehringer, U., Stymne, S., and Heinz, E. (2000) Eur. J. Biochem. 267, 3801-3811
27. Girke, T., Schmidt, H., Zahringer, U., Reski, R. & Heinz, E. (1998) Plant J. 15, 39-48
28. Sayanova, O., Smith, M. A., Lapinskas, P., Stobart, K. A., Dobson, G., Christie, W. W., Shewry, P. R. & Napier, J. A. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 4211-4216
29. Sperling, P., Zahringers, U. & Heinz, E. (1998) J. Biol. Chem. 273, 28590-28596
30. Leonard, A. E., Kelder, B., Bobik, E. G., Chuang, L., Parker-Barnes, J. M., Thurmond, J. M., Kroeger, P. E., Kopchick, J. J., Huang, Y. & Mukerji, P. (2000) Biochem. J. 347, 719-724
31. Napier, J. A., Hey, S. J., Lacey, D. J. & Shewry, P. R. (1998) Biochem. J. 330, 611-614
32. Michaelson, L. V., Napier, J. A., Lewis, M., Griffiths, G., Lazarus, C. M. & Stobart, K. A. (1998) FEBS Lett. 439, 215-218
33. Wallis, J. G. & Browse, J. (1999) Arch. Biochem. Biophys. 365, 307-316
34. Sakuradani, E., Kobayashi, M. & Shimizu, S. (1999) Gene (Amst.) 238, 445-453
35. Parker-Barnes, J. M., Das, T., Bobik, E., Leonard, A. E., Thurmond, J. M., Chaung, L., Huang, Y. & Mukerji, P. (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 8284-8289
36. Beaudoin, F., Michaelson, L. V., Hey, S. J., Lewis, M. J., Shewry, P. R., Sayanova, O. & Napier, J. A. (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 6421-6426
37. Leonard, A. E., Bobik, E., Dorado, J., Kroeger, P. E., Chuang, L., Thurmond, J. M., Parker-Barnes, J. M., Das, T., Huang, Y. & Mukerji, P. (2000) Biochem. J. 350, 765-770
38. Mohammed, B. S., Sankarappa, S., Geiger, M. & Sprecher, H. (1995) Arch. Biochem Biophys. 317, 179-184
39. Infante, J. P. & Huszagh, V. A. (2000) FEBS Lett. 468, 1-5
40. Luthria, D. L., Mohammed, B. S. & Sprecher, H. (1996) J. Biol. Chem. 271, 16020-16025
41. Sprecher, H. (1996) Nutrition 12, S5-7
42. Mohammed, B. S., Luthria, D. L., Bakousheva, S. P. & Sprecher, H. (1997) Biochem. J 326, 425-430
43. Sprecher, H., Chen, Q. & Yin, F. Q. (1999) Lipids 34, S153-6
44. Innis, S. M., Sprecher, H., Hachey, D., Edmond, J. & Anderson, R. E. (1999) Lipids 34, 139-149
45. Infante, J. P. & Huszagh, V. A. (1998) FEBS Lett. 431, 1-6
46. Infante, J. P. & Huszagh, V. A. (1997) Mol. Cell. Biochem. 168, 101-115