Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of Δ6-desaturated fatty acids in transgenic tobacco

Proceedings of the National Academy of Sciences of the United States of America

Volumn 94, Issue 8, 1997, Pages 4211-4216

  Sayanova, Olga ^a   Smith, Mark A ^a   Lapinskas, Peter ^b   Stobart, A Keith ^a   Dobson, Gary ^c   Christie, William W ^c   Shewry, Peter R ^a   Napier, Johnathan A ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

^b Weyvern House   (United Kingdom)

^c SCOTTISH CROP RESEARCH INSTITUTE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BORAGO OFFICINALIS EXTRACT; GAMMA LINOLENIC ACID; OCTADECATETRANOIC ACID; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL TRANSFORMATION; ENZYME ANALYSIS; FATTY ACID DESATURATION; FATTY ACID SYNTHESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; TOBACCO;

AMINO ACID SEQUENCE; CYTOCHROMES B5; DNA, COMPLEMENTARY; FATTY ACID DESATURASES; GENE EXPRESSION; LINOLEOYL-COA DESATURASE; MOLECULAR SEQUENCE DATA; PLANTS, GENETICALLY MODIFIED; PLANTS, TOXIC; SEQUENCE ALIGNMENT; TOBACCO;

BORAGINACEAE; BORAGO OFFICINALIS; EMBRYOPHYTA; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; OENOTHERA; PRIMULA;

EID: 0030890581     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.94.8.4211     Document Type: Article

References (37)

1. Horrobin, D. F. (1992) Prog. Lipid Res. 31, 163-194.
2. Horrobin, D. F. (1990) Rev. Contemp. Pharmacother. 1, 1-45.
3. Gunstone, F. D. (1992) Prog. Lipid Res. 31, 145-161.
4. Fieldsend, A. F. (1995) Biologist 42, 203-207.
5. Heinz, E. (1993) in Lipid Metabolism in Plants, ed. Moore, T. S. (CRC, Boca Raton, FL), pp. 34-89.
6. Stymne, S. & Stobart, A. K. (1993) in Seed Storage Compounds, eds. Shewry, P. R. & Stobart, A. K. (Oxford Univ. Press, Oxford), pp. 96-114.
7. Griffiths, G., Brechany, E. Y., Christie, W. W., Stymne, S. & Stobart A. K. (1989) in Biological Role of Plant Lipids, eds. Biacs, P. A., Gruiz, K. & Kremmer, T. (Plenum, New York), pp. 151-154.
8. Smith, M. A., Cross, A. R., Jones, O. T. G., Griffiths, W. T., Stymne, S. & Stobart, A. K. (1990) Biochem. J. 272, 23-29.
9. Griffiths, G., Brechany, E. Y., Jackson, F. M., Christie, W. W., Stymne S. & Stobart, A. K. (1996) Photochemistry 43, 381-386.
10. Arondel, V., Lemieux, B., Hwang, I., Gibson, S., Goodman, H. & Somerville, C. R. (1992) Science 258, 1353-1355.
11. Okuley, J., Lightner, J., Feldmann, K., Yadav, N., Lark, E. & Browse, J. (1994) Plant Cell 6, 147-158.
12. Lederer, F. (1994) Biochimie 76, 674-692.
13. Kerns, E. V., Hugly, S. & Somerville, C. R. (1991) Arch. Biochem. Biophys. 284, 431-436.
14. Napier, J. A., Smith, M. A., Stobart, A. K. & Shewry, P. R. (1995) Planta 197, 200-202.
15. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab. Press, Plainview, NY), 2nd Ed.
16. Devereux, J., Haeberli, P. & Smithies, O. (1984) Nucleic Acids Res. 12, 387-395.
17. Smith, M. A., Stobart, A. K., Shewry, P. R. & Napier, J. A. (1994) Plant Mol. Biol. 25, 527-537.
18. Zakai, N., Ballas, N., Hershkovitz, M., Broido, S., Ram, R. & Loyter, A. (1993) Plant Mol. Biol. 21, 823-830.
19. Gallie, D. R., Lucas, W. J. & Walbot, V. (1989) Plant Cell 1, 301-311.
20. Bevan, M. (1984) Nucleic Acid Res. 12, 8711-8722.
21. Draper, J., Scott, R., Armatige, P. & Walden, R. (1988) Plant Genetic Transformation and Gene Expression: A Laboratory Manual (Blackwell Scientific, Oxford).
22. Bligh, E. G. & Dyer, W. J. (1959) Can. J. Biochem. Physiol. 37, 911-917.
23. Fay, L. & Richli, U. (1991) J. Chromatogr. 541, 89-98.
24. Traitler, H., Willie, H. J. & Studer, A. (1988) J. Am. Oil Chem. Soc. 65, 755-760.
25. Zhang, J. Y., Yu, O. T., Liu, B. N. & Huang, Z. H. (1988) Biomed. Environ. Mass Spectrom. 15, 33-44.
26. Shanklin, J., Whittle, E. & Fox, B. G. (1994) Biochemistry 33, 12787-12794.
27. Stymne, S. & Stobart, A. K. (1986) Biochem. J. 24, 385-393.
28. Reddy, A. S., Nuccio, M. L., Gross, L. M. & Thomas, T. L. (1993) Plant Mol. Biol. 22, 293-300.
29. Sperling, P., Schmidt, H. & Heinz, E. (1995) Eur. J. Biochem. 232, 798-805.
30. Avelange-Macherel, M.-H., Macherel, D., Wada, H. & Murata, N. (1995) FEBS Lett. 361, 111-114.
31. van de Loo, F. J., Broun, P., Turner, S. & Somerville C. (1995) Proc. Natl. Acad. Sci. USA 92, 6743-6747.
32. Smith, M. A., Jonsson, L., Stymne, S. & Stobart, A. K. (1992) Biochem. J. 287, 141-144.
33. Smith, M. A., Napier, J. A., Stymne, S., Tatham, A. S., Shewry, P. R. & Stobart, A. K. (1994) Biochem. J. 303, 73-79.
34. Napier, J. A. (1995) in Methods in Molecular Biology, ed. Jones, H. (Humana, Totowa, NJ), Vol. 49, pp. 369-376.
35. Mitchell, A. G. & Martin, C. E. (1995) J. Biol. Chem. 270, 29766-29772.
36. Reddy, A. V. & Thomas, T. L. (1996) Nat. Biotechnol. 14, 639-642.
37. Salunkhe, D. K., Chavan, J. K., Adsule, R. N., Kadam, S. S. (1991) World Oilseeds - Chemistry, Technology and Utilization (Van Nostrand Reinhold, New York).