Identification of Δ5-desaturase from Mortierella alpina by heterologous expression in bakers' yeast and canola

Journal of Biological Chemistry

Volumn 273, Issue 45, 1998, Pages 29360-29366

  Knutzon, Deborah S ^b   Thurmond, Jennifer M ^a   Huang, Yung Sheng ^a   Chaudhary, Sunita ^a   Bobik Jr , Emil G ^a   Chan, George M ^b   Kirchner, Stephen J ^a   Mukerji, Pradip ^a  

^a ABBOTT LABORATORIES   (United States)

^b Calgene LLC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LONG CHAIN FATTY ACID; POLYUNSATURATED FATTY ACID; ACYL COENZYME A DESATURASE; COMPLEMENTARY DNA; DELTA 15 DESATURASE; DELTA-15 DESATURASE; PRIMER DNA; RECOMBINANT PROTEIN;

ARTICLE; FATTY ACID SYNTHESIS; GENE ISOLATION; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; AMINO ACID SEQUENCE; ENZYMOLOGY; GENETICS; MASS FRAGMENTOGRAPHY; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MORTIERELLA; NUCLEOTIDE SEQUENCE; PLANT SEED; SEQUENCE HOMOLOGY; TRANSGENIC PLANT;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; DNA, COMPLEMENTARY; FATTY ACID DESATURASES; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; MORTIERELLA; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SEEDS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0032491316     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.273.45.29360     Document Type: Article

References (33)

1. Horrobin, D. F. (1992) Prog. Lipid Res. 31, 163-194
2. Gunstone, F. D., Harwood, J. L., and Padley, F. B. (1994) The Lipid Handbook, 2nd Ed., Chapman & Hill, London
3. Browse, J., and Somerville, C. (1991) Annu. Rev. Plant Physiol. Plant Mol. Biol. 42, 467-506
4. Okuley, J., Lightner, J., Feldmann, K., Yadav, N., Lark, E., and Browse, J. (1994) Plant Cell 6, 147-158
5. Arondel, V., Lemieux, B., Hwang, I., Gibson, S., Goodman, H. M., and Somerville, C. R. (1992) Science 258, 1353-1355
6. Spychalla, J. P., Kinney, A. J., and Browse, J. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 1142-1147
7. Reddy, A. S., Nuccio, M. L., Gross, L. M., and Thomas T. L. (1993) Plant Mol. Biol. 22, 293-300
8. Murata, N., Deshnium, P., and Tasaka, Y. (1996) in Gamma-linolenic Acid, Metabolism and Its Roles in Nutrition and Medicine (Huang, Y., and Milles, D. E., eds) pp. 22-32, AOC Press, Champaign, IL
9. Sayanova, O., Smith, M. A., Lapinskas, P., Stobart, A. K., Dobson, G., Christie, W. W., Shewry, P. R., and Napier, J. A. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 4211-4216
10. Shanklin, J., Whittle, E., and Fox, B. G. (1994) Biochemistry 33, 12787-12794
11. Napier, J. A., Hey, S. J., Lacey, D. J., and Shewry, P. (1998) Biochem. J. 330, 611-614
12. Cadena, D. L., Kurten, R. C., and Gill, G. N. (1997) Biochemistry 36, 6960-6967
13. Yamada, H., Shimizu, S., and Shinmen. Y. (1987) Agric. Biol. Chem. 51, 785-790
14. Shinmen, Y., Shimizu, S., Akimoto, K., Kawashima, H., and Yamada, H. (1989) Appl. Microbiol. Biotechnol. 31, 11-16
15. Jareonkitmongkol, S., Shimizu, S., and Yamada, H. (1992) J. Gen. Microbiol. 138, 997-1002
16. Hoveland, P., Flick, J., Johnston, M., and Sclafani, R. A. (1989) Gene (Amst.) 83, 57-64
17. Hoge, J. H. C., Springer, J., Zantinge, B., and Wessels, J. G. H. (1982) Exp. Mycol. 6, 225-232
18. Knutzon, D. S., Lardizabal, K. D., Nelsen, J. S., Bleibaum, J. L., Davies, H. M., and Metz, J. G. (1995) Plant Physiol. 109, 999-1006
19. Stearns, T., Ma, H., and Botstein, D. (1990) Methods Enzymol. 185, 280-297
20. Kridl, J. C., McCarter, D. W., Rose, R. E., Scherer, D. E., Knutzon, D. S., Radke, S. E., and Knauf, V. C. (1991) Seed Sci. Res. 1, 209-219
21. McBride, K. E., and Summerfelt, K. R. (1990) Plant Mol. Biol. 14, 269-276
22. Radke, S. E., Andrews, B. M., Moloney, M. M., Crouch, M. L., Kridl, J. C., and Knauf, V. K. (1987) Theor. Appl. Genet. 75, 685-694
23. Huang, Y. S., Smith, R. S., Redden, P. R., Cantrill, R. C., and Horrobin, D. F. (1991) Biochim. Biophys. Acta 1082, 319-327
24. Claros, M. G., and von Heijne, G. (1994) Comput. Appl. Biosci. 10, 685-686
25. Sperling, P., Schmidt, H., and Heinz, E. (1995) Eur. J. Biochem. 232, 798-805
26. Smith, M. A., Cross, A. R., Jones, O. T. G., Griffiths, W. T., Stymne, S., and Stobart, K. (1990) Biochem. J. 272, 23-29
27. Smith, M. A., Jonsson, L., Stymne, S., and Stobart K. (1992) Biochem. J. 287, 141-144
28. Takagi, T., and Itabashi, Y. (1982) Lipids 17, 716-723
29. Wolff, R. L. (1997) INFORM 8, 116-119
30. Browse, J., McConn, M., James, D., and Miquel, M. (1993) J. Biol. Chem. 268, 16345-16351
31. Truan, G., Epinat, J. C., Rougeulle, C., Cullin, C., and Pompon, D. (1994) Gene (Amst.) 142, 123-127
32. Smith, M. A., Stobart, A. K., Shewry, P. R., and Napier, J. A. (1994) Plant Mol. Biol. 25, 527-537
33. Yoo, M., and Steggles, A. W. (1988) Biochem. Biophys. Res. Commun. 156, 576-580