Functional analysis of a conserved aspartate D218 in Cephalosporium acremonium isopenicillin N synthase

FEMS Microbiology Letters

Volumn 157, Issue 1, 1997, Pages 137-140

  Loke, Paxton ^a   Sim, Janet ^a   Sim, Tiow Suan ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Cephalosporium acremonium; Isopenicillin N synthase; Site directed mutagenesis

Indexed keywords

ISOPENICILLIN N SYNTHETASE;

ANTIBIOTIC BIOSYNTHESIS; ARTICLE; CEPHALOSPORIUM ACREMONIUM; ENZYME ACTIVITY; PRIORITY JOURNAL; REACTION ANALYSIS; SITE DIRECTED MUTAGENESIS;

ACREMONIUM; ASPARTIC ACID; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; MUTAGENESIS, SITE-DIRECTED; OXIDOREDUCTASES; PENICILLINS;

ACREMONIUM CHRYSOGENUM; ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); CEPHALOSPORIUM; STREPTOMYCES JUMONJINENSIS; UNCULTURED ACTINOMYCETE;

EID: 0031408344     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(97)00466-7     Document Type: Article

References (15)

1. Baldwin, J.E. and Abraham, E.P. (1988) The biosynthesis of penicillins and cephalosporins. Nat. Prod. Rep. 5, 129-145.
2. Perry, D., Abraham, E.P. and Baldwin, J.E. (1988) Factors affecting the isopenicillin N synthetase reaction. Biochem. J. 255, 345-351.
3. Chen, V.J., Orville, A.M., Harpel, M.R., Frolik, C.A., Sureus, K.K., Munck, E. and Lipscomb, J.D. (1989) Spectroscopic studies of Isopenicillin N synthase. J. Biol. Chem. 264, 21677-21681.
4. Ming, L.-J., Que, L. Jr., Kriauciunas, A., Frolik, C.A. and Chen, V.J. (1991) NMR studies of the active site of isopenicillin N synthase, a non-heme iron(II) enzyme. Biochemistry 30, 11653-11659.
5. Roach, P.L., Clifton, I.J., Fulop, V., Harlos, K., Barton, G.J., Hadju, J., Anderson, I., Schofield, C.J. and Baldwin, J.E. (1995) Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes. Nature 375, 700-704.
6. Roach, P.L., Clifton, I.J., Hensgens, C.M.H., Shibata, N., Schofield, C.J., Hadju, J. and Baldwin, J.E. (1997) Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation. Nature 387, 827-830.
7. Tan, S.H.D. and Sim, T.S. (1996) Functional analysis of conserved histidine residues in Cephalosporium acremonium Isopenicillin N synthase by site-directed mutagenesis. J. Biol. Chem. 271, 889-894.
8. Borovok, I., Landman, O., Kreisberg-Zakarin, R., Aharonowitz, Y. and Cohen, G. (1996) Ferrous active site of isopenicillin N synthase: genetic and sequence analysis of the endogenous ligands. Biochemistry 35, 1981-1987.
9. Devereux, J., Haeberli, P. and Smithies, O. (1984) A comprehensive set of sequence analysis programmes for VAX. Nucleic Acids Res. 12, 387-395.
10. Chou, P.Y. and Fasman, G.D. (1974) Prediction of protein conformation. Biochemistry 13, 222-245.
11. Garnier, J., Osguthorpe, D.J. and Robson, B. (1978) Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J. Mol. Biol. 120, 97-120.
12. Sim, B.J., Tan, D.S.H., Liu, X. and Sim, T.S. (1996) Production of high levels of soluble recombinant Streptomyces clavuligerus isopenicillin N synthase in Escherichia coli. J. Mol. Catal. B Enzym. 2, 71-83.
13. Cohen, G., Shiffman, D., Mevarech, M. and Aharonowitz, Y. (1990) Microbial isopenicillin N synthase genes: structure, function, diversity and evolution. Trends Biotechnol. 8, 105-111.
14. Weigel, B.J., Burgett, S.G., Chen, V.J., Skatrud, P.L., Frolik, C.A., Queener, S.W. and Ingolia, T.D. (1988) Cloning and expression in Escherichia coli of Isopenicillin N synthetase genes from Streptomyces lipmanii and Aspergillus nidulans. J. Bacteriol. 170, 3817-3826.
15. Shaw, J.F., Chou, Y.S., Chang, R.C. and Yang, S.F. (1996) Characterization of the ferrous ion binding sites of apple 1-aminocyclopropane-1-carboxylate oxidase by site-directed mutagenesis. Biochem. Biophys. Res. Commun. 225, 697-700.