Site-directed mutagenesis of arginine-89 supports the role of its guanidino side-chain in substrate binding by Cephalosporium acremonium isopenicillin N synthase

FEMS Microbiology Letters

Volumn 179, Issue 2, 1999, Pages 423-429

  Loke, Paxton ^a   Sim, Tiow Suan ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Cephalosporium acremonium; Isopenicillin N synthase; Site directed mutagenesis; Substrate binding

Indexed keywords

ISOPENICILLIN N SYNTHETASE;

ARTICLE; CATALYSIS; CEPHALOSPORIUM ACREMONIUM; CYCLIZATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME BINDING; ENZYME SPECIFICITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS;

ACREMONIUM; AMINO ACID SEQUENCE; ARGININE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; OXIDOREDUCTASES; PROTEIN STRUCTURE, SECONDARY; SUBSTRATE SPECIFICITY;

ACREMONIUM CHRYSOGENUM; ASPERGILLUS; CEPHALOSPORIUM; EMERICELLA NIDULANS;

EID: 0032863509     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(99)00445-0     Document Type: Article

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