Structural basis for SRY-dependent 46-X,Y sex reversal: Modulation of DNA bending by a naturally occurring point mutation

Journal of Molecular Biology

Volumn 312, Issue 3, 2001, Pages 481-499

  Murphy, Elizabeth C ^a   Zhurkin, Victor B ^b   Louis, John M ^a   Cornilescu, Gabriel ^a   Clore, G Marius ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

46X,Y sex reversal; DNA bending mutation; Multidimensional NMR; SRY DNA complex

Indexed keywords

CURVED DNA; HIGH MOBILITY GROUP PROTEIN; NUCLEAR PROTEIN; TESTIS DETERMINING FACTOR; UNCLASSIFIED DRUG;

ARTICLE; DNA PROTEIN COMPLEX; DNA SEQUENCE; DNA STRUCTURE; HUMAN; HYDROGEN BOND; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POINT MUTATION; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SEX TRANSFORMATION; X CHROMOSOME; Y CHROMOSOME;

EID: 0035929239     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4977     Document Type: Article

References (86)

1. Transcriptional activation: A complex puzzle with few easy pieces
2. Higher-order nucleoprotein complexes in transcription: Analogies with site-specific recombination
3. Minor groove-binding architectural proteins: Structure, function and DNA recognition
4. Flexing DNA: HMG-box proteins and their partners
5. Structure and function of the interferon-β enhanceosome
6. Transcription regulation by repressosome and by RNA polymerase contact
7. Non-sequence-specific DNA recognition: A structural perspective
8. Floppy SOX: Mutual induced fit in HMG (high-mobility group) box-DNA recognition
9. Ancestry and diversity of the HMG box superfamily
10. HMG domain proteins: Architectural elements in the assembly of nucleoprotein structures
11. High-mobility-group chromosomal proteins: Architectural components that facilitate chromatin function
12. Molecular basis of human 46X,Y sex reversal revealed from the three-dimensional solution structure of the human SRY-DNA complex
13. Structural basis for DNA bending by the architectural transcription factor LEF-1
14. The structure of a chromosomal high mobility group protein-DNA complex reveals sequence-neutral mechanisms important for non-specific DNA recognition
15. Solution structure of the HMG protein NHP6A and its interaction with DNA reveals the structural determinants for non-specific binding
16. Basis for recognition of cisplatin-modified DNA by high-mobility-group proteins
17. SRY and sex determination in mammals
18. Sry and Sox9: Mammalian testis-determining genes
19. Gene interactions in gonadal development
20. Whatever happened to SRY?
21. SRY recognizes conserved DNA sites in sex-specific promoters
22. Molecular basis of mammalian sexual determination: Activation of Müllerian inhibiting substance gene expression by SRY
23. Analysis of the role of Amh and Fra-1 in the Sry regulatory pathway
24. A male-specific role for Sox9 in vertebrate sex determination
25. Sox9 expression during gonadal development implies a conserved role for the gene in testis differentiation in mammals and birds
26. A gene from the human sex determining region encodes a protein with homology to a conserved DNA-binding motif
27. Two independent nuclear localization signals are present in the DNA-binding high-mobility group domains of SRY and SOX9
28. The HMG box of SRY is a calmodulin binding domain
29. Understanding 46X,Y sex reversal at the atomic level
30. Sex-reversing mutations affect the architecture of SRY-DNA complexes
31. Structures of larger proteins in solution: Three- and four-dimensional heteronuclear NMR spectroscopy
32. Determining structures of larger proteins and protein complexes by NMR
33. Methodological advances in protein NMR
34. Direct measurement of distances and angles in biomolecules by NMR in dilute liquid crystalline medium
35. 13C couplings in the structure determination of magnetically oriented macromolecules in solution
36. Impact of residual dipolar couplings on the accuracy of NMR structures determined from a minimal number of NOE restraints
37. R-factor, free R and complete cross-validation for dipolar coupling refinement of NMR structures
38. rphosphoryl transfer complex between enzyme I and HPr
39. Accurate and rapid docking of protein-protein complexes on the basis of intermolecular nuclear Overhauser enhancement data and dipolar couplings by rigid body minimization
40. Glucose of the Escherichia coli phosphoenolpyruvate: Sugar phosphotransferase system
41. A role for CH-O interactions in protein-DNA recognition
42. Mechanisms of DNA bending in the free state and in the nucleosome
43. DNA sequence-dependent deformability deduced from protein-DNA crystal complexes
44. Steric interactions in polyribonucleotides: A virtual bond model
45. Bent DNA: Visualization of a base-paired and stacked A-B conformational junction
46. Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal
47. DNA-binding properties of the HMG domain of the lymphoid-specific transcriptional regulator LEF-1
48. The DNA sequence specificity of HMG boxes lies in the minor wing of the structure
49. Functional and structural studies of wild-type SOX9 and mutations causing campomelic displasia
50. Empirical estimation of protein-induced bending angles: Applications to lambda site-specific recombination complexes
51. Relating independent measures of DNA curvature: Electrophoretic anomaly and cyclization efficiency
52. DNA bending due to specific p53 and p53 core domain-DNA interactions visualized by electron microscopy
53. Modeling DNA deformations
54. Preparation of uniformly isotope-labeled DNA oligonucleotides for NMR spectroscopy
55. Bending of DNA by gene-regulatory proteins: Construction and use of a DNA bending vector
56. NMRPipe: A multidimensional spectral processing system based on UNIX PIPES
57. A common sense approach to peak picking in two-, three- and four-dimensional spectra using automatic computer analysis of contour diagrams
58. Determination of three-dimensional structures of proteins and nucleic acids in solution by nuclear magnetic resonance spectroscopy
59. Measurement of homo- and heteronuclear J couplings from quantitative J correlation
60. Protein backbone angle restraints from searching a database for protein chemical shift and sequence homology
61. 31P couplings in nucleic acids and protein-nucleic acid complexes by quantitative J correlation spectroscopy
62. Measurement of J and dipolar couplings from simplified two-dimensional NMR spectra
63. 13C J couplings in staphylococcal nuclease
64. Solution structure of cyanovirin-N, a potent HW-inactivating protein
65. The NMR structure of a DNA dodecamer in an aqueous dilute liquid crystalline phase
66. A robust method for determining the magnitude of the fully asymmetric alignment tensor of oriented macromolecules in the absence of structural information
67. The solution structure of a specific GAGA factor-DNA complex reveals a modular binding mode
68. Conformation and dynamics of DNA and protein-DNA complexes by P-31 NMR
69. 1H NMR
70. Determination of three-dimensional structures of proteins by simulated annealing with interproton distance restraints: Application to crambin, potato carboxypeptidase inhibitor and barley serine proteinase inhibitor 2
71. New methods of structure refinement for macromolecular structure determination by NMR
72. Internal coordinates for molecular dynamics and minimization in structure determination and refinement
73. Direct refinement against proton-proton dipolar couplings in NMR structure determination of macromolecules
74. Direct refinement against residual dipolar couplings in the presence of rhombicity of unknown magnitude
75. Solution structure of the MEF2A-DNA complex: Structural basis for the modulation of DNA bending and specificity by MADS-box transcription factors
76. Source of and solutions to problems in the refinement of protein NMR structures against torsion angle potentials of mean force
77. Improving the accuracy of NMR structures of DNA by means of a database potential of mean force describing base-base positional interactions
78. The solution structure of a fungal AREA protein-DNA complex: An alternative binding mode for the basic carboxyl tail of GATA factors
79. The solution structure of the Leu22Æval mutant of the AREA DNA binding domain complexed with a TGATA core element defines a role for hydrophobic packing in the determination of specificity
80. The definition of generalized helicoidal parameters and of axis curvature for irregular nucleic acids
81. B-DNA twisting correlates with base-pair morphology
82. The VMD-XPLOR visualization package for NMR structure refinement
83. MOL-MOL: A program for display and analysis of macromolecular structures
84. Protein folding and association: Insights into interfacial and thermodynamic properties of hydrocarbons
85. PROCHECK: A program to check the stereochemical quality of protein structures