Cytoarchitecture and physical properties of cytoplasm: Volume, viscosity, diffusion, intracellular surface area

International Review of Cytology

Volumn 192, Issue , 1999, Pages 189-221

  Luby Phelps, Katherine ^a  

^a UNIVERSITY OF TEXAS AT DALLAS   (United States)

Author keywords

Cytoplasm; Diffusion; Particle Transport; Partitioning; Viscosity

Indexed keywords

CELL PROTEIN; ENZYME; WATER;

AQUEOUS SOLUTION; CELL SURFACE; CELL VACUOLE; CELL VOLUME; CYTOARCHITECTURE; CYTOPLASM; DIFFUSION; PHASE SEPARATION; PRIORITY JOURNAL; REVIEW; STEREOSPECIFICITY; VISCOSITY;

EID: 0033991590     PISSN: 00747696     EISSN: None     Source Type: Book Series    
DOI: 10.1016/s0074-7696(08)60527-6     Document Type: Article

References (145)

1. Abercrombie, M., Heaysman, J. E. M., and Pegrum, S. M. (1971). The locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamella. Exp. Cell Res. 67, 359-367.
2. Agutter, P. S., Malone, P. C., and Wheatley, D. N. (1995). Intracellular transport mechanisms: a critique of diffusion theory. J. Theor. Biol. 176, 261-272.
3. Ainger, K., Avossa, D., Morgan, F., Hill, S. J., Barry, C., Barbarese, E., and Carson, J. H. (1993). Transport and localization of exogenous myelin basic protein mRNA microinjected into oligodendrocytes. J. Cell Biol. 123, 431-441.
4. Ainger, K., Avossa, D., Diana, A. S., Barry, C., Barbarese, E., and Carson, J. H. (1997). Transport and localization elements in myelin basic protein mRNA. J. Cell Biol. 138, 1077-1087.
5. Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K., and Watson, J. D. (1994). "Molecular Biology of the Cell," 3rd ed. Garland Publishing, New York.
6. Arnold, H., and Pette, D. (1968). Binding of glycolytic enzymes to structure proteins of the muscle. Eur. J. Biochem. 6, 163-171.
7. Arrio-Dupont, M., Cribier, S., Foucault, G., Devaux, P. F., and Dalbis, A. (1996). Diffusion of fluorescently labeled macromolecules in cultured muscle cells. Biophys. J. 70, 2327-2332.
8. Arrio-Dupont, M., Foucault, G., Vacher, M., Douhou, A., and Cribier, S. (1997). Mobility of creatine phosphokinase and beta-enolase in cultured muscle cells. Biophys. J. 73, 2667-2673.
9. Axelrod, D., Koppel, D. E., Schlessinger, J., Elson, E., and Webb, W. W. (1976). Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys. J. 16, 1055-1069.
10. Bassell, G., and Singer, R. H. (1997). mRNA and cytoskeletal filaments. Curr. Opin. Cell Biol. 9, 109-115.
11. Bereiter-Hahn, J., Stubig, C., and Heymann, V. (1995). Cell cycle-related changes in F-actin distribution are correlated with glycolytic activity. Exp. Cell Res. 218, 551-560.
12. Bicknese, S., Periasamy, N., Shohet, S. B., and Verkman, A. S. (1993). Cytoplasmic viscosity near the cell plasma membrane: Measurement by evanescent field frequency-domain microfluorimetry. Biophys. J. 65, 1272-1282.
13. Bray, D., and Thomas, C. (1975). The actin content of fibroblasts. Biochem. J. 147, 221-228.
14. Bridgman, P. C., Kachar, B., and Reese, T. S. (1986). The structure of cytoplasm in directly frozen cultured cells. II. Cytoplasmic domains associated with organelle movements. J. Cell Biol. 102, 1510-1521.
15. Burke, N. V., Han, W., Li, D., Takimoto, K., Watkins, S. C., and Levitan, E. S. (1997). Neuronal peptide release is limited by secretory granule mobility. Neuron. 19, 1095-1102.
16. Cameron, I. L., Hardman, W. E., Fullerton, G. D., Miseta, A., Koszegi, T., Ludany, A., and Kellermayer, M. (1996). Maintenance of ions, proteins and water in lens fiber cells before and after treatment with non-ionic detergents. Cell Biol. Int. 20, 127-137.
17. Cameron, I. L., Kanal, K. M., Keener, C. R., and Fullerton, G. D. (1997). A mechanistic view of the non-ideal osmotic and motional behavior of intracellular water. Cell Biol. Int. 21, 99-113.
18. Carlier, M. F., and Pantaloni, D. (1997). Control of actin dynamics in cell motility. J. Mol. Biol. 269, 459-467.
19. Chang, T., Kim, H., and Yu, H. (1987). Diffusion through coarse meshes. Macromolecules 20, 2629-2631.
20. Chou, Y. H., Skalli, O., and Goldman, R. D. (1997). Intermediate filaments and Cytoplasmic networking: New connections and more functions. Curr. Opin. Cell Biol. 9, 49-53.
21. Clegg, J. S. (1984). Properties and metabolism of the aqueous cytoplasm and its boundaries. Am. J. Physiol. 246, R133-R151.
22. Clegg, J. S. (1986). L-929 cells under hyperosmotic conditions: Volume changes. Cell. Physiol. 129, 367-374.
23. Clegg, J. S., and Jackson, S. A. (1988). Glycolysis in permeabilized L-929 cells. Biochem. J. 255, 335-344.
24. Clegg, J. S., and Jackson, S. A. (1990). Glucose metabolism and the channeling of glycolytic intermediates in permeabilized L-929 cells. Arch. Biochem. Biophys. 278, 452-460.
25. Clegg, J. S., Jackson, S. A., and Fendl, K. (1990). Effects of reduced cell volume and water content on glycolysis in L-929 cells. J. Cell. Physiol. 142, 386-391.
26. Cohen, N. S. (1996). Intracellular localization of the mRNAs of argininosuccinate synthetase and argininosuccinate lyase around liver mitochondria, visualized by high-resolution in situ reverse transcription-polymerase chain reaction. J. Cell Biochem. 61, 81-96.
27. Cohen, N. S., and Kuda, A. (1996). Argininosuccinate synthetase and argininosuccinate lyase are localized around mitochondria: an immunocytochemical study. J. Cell Biochem. 60, 334-340.
28. Condeelis, J. (1995). Elongation factor 1 alpha, translation and the cytoskeleton. Trends Biochem. Sci. 20, 169-170.
29. Cooke, R., and Kuntz, I. D. (1974). The properties of water in biological systems. Annu. Rev. Biophys. 3, 95-126.
30. Cunningham, C. C., Leclerc, N., Flanagan, L. A., Lu, M., Janmey, P. A., and Kosik, K. S. (1997). Microtubule-associated protein 2c reorganizes both microtubules and microfilaments into distinct cytological structures in an actin-binding protein-280-deficient melanoma cell line. J. Cell Biol. 136, 845-857.
31. Eckert, B. S. (1986). Alteration of the distribution of intermediate filaments in PtK1 cells by acrylamide. II: Effect on the organization of cytoplasmic organelles. Cell Motil. Cytoskel. 6, 15-24.
32. Elson, E. L., and Qian, H. (1989). Interpretation of fluorescence correlation spectroscopy and photobleaching recovery in terms of molecular interactions. Methods Cell Biol. 30, 307-332.
33. Eriksson, J. E., Brautigan, D. L., Vallee, R., Olmsted, J., Fujiki, H., and Goldman, R. D. (1992). Cytoskeletal integrity in interphase cells requires protein phosphatase activity. Proc. Natl. Acad. Sci. U.S.A. 89, 11093-11097.
34. Felder, S., and Kam, Z. (1994). Human neutrophil motility: Time-dependent three-dimensional shape and granule diffusion. Cell Motil. Cytoskel. 28, 285-302.
35. Foster, K. R., Resing, H. A., and Garroway, A. N. (1976). Bounds on "bound water": Transverse nuclear magnetic resonance relaxation in barnacle muscle. Science 194, 324-326.
36. Fulton, A. B. (1982). How crowded is the cytoplasm? Cell (Cambridge, Mass.) 30, 345-347.
37. Fulton, A. B., and L'Ecuyer, T. (1993). Cotranslational assembly of some cytoskeletal proteins: Implications and prospects. J. Cell Sci. 105, 867-871.
38. Fushimi, K., and Verkman, A. S. (1991). Low viscosity in the aqueous domain of cell cytoplasm measured by picosecond polarization microfluorimetry. J. Cell Biol. 112, 719-725.
39. Gershon, N. D., Porter, K. R., and Trus, B. L. (1985). The cytoplasmic matrix: Its volume and surface area and the diffusion of molecules through it. Proc. Natl. Acad. Sci. U.S.A. 82, 5030-5034.
40. Goodsell, D. S. (1991). Inside a living cell. Trends Biochem. Sci. 16, 203-206.
41. Goodsell, D. S. (1993). "The Machinery of Life" Springer-Verlag, New York.
42. Green, L. (1968). Mechanism of movements of granules in melanocytes of fundulus heteroclitus. Proc. Natl. Acad. Sci. U.S.A. 59, 1179-1186.
43. Hailing, P. J. (1989). Do the laws of chemistry apply to living cells? Trends Biochem. Sci. 14, 317-318.
44. Hamill, D., Davis, J., Drawbridge, J., and Suprenant, K. A. (1994). Polyribosome targeting to microtubules: Enrichment of specific mRNAs in a reconstituted microtubule preparation from sea urchin embryos. J. Cell Biol. 127, 973-984.
45. Hashimoto, Y., and Shinozaki, N. (1988). Measurement of cytoplasmic viscosity by fluorescence polarization in phytohemagglutinin-stimulated and unstimulated human peripheral lymphocytes. J. Histochem. Cytochem. 36, 609-613.
46. Herzfeld, J. (1996). Entropically driven order in crowded solutions: From liquid crystals to cell biology. Acc. Chem. Res. 29, 31-37.
47. Heuser, J. E., and Kirschner, M. W. (1980). Filament organization revealed in platinum replicas of freeze-dried cytoskeletons. J. Cell Biol. 86, 212-234.
48. Hiller, G., and Weber, K. (1978). Radioimmunoassay for tubulin: A quantitative comparison of the tubulin content of different established tissue culture cells and tissues. Cell (Cambridge, Mass.) 14, 795-804.
49. Holwell, T. A., Schweitzer, S. C., and Evans, R. M. (1997). Tetracycline regulated expression of vimentin in fibroblasts derived from vimentin null mice. J. Cell Sci. 110, 1947-1956.
50. Hoppeler, H., Hudlicka, O., and Uhlmann, E. (1987). Relationship between mitochondria and oxygen consumption in isolated cat muscles. J. Physiol. (London) 385, 661-675.
51. Hou, L., Lanni, F., and Luby-Phelps, K. (1990). Tracer diffusion in F-actin and Ficoll mixtures. Toward a model for cytoplasm. Biophys. J. 58, 31-43.
52. Hsu, E. W., Aiken, N. R., and Blackband, S. J. (1996). Nuclear magnetic resonance microscopy of single neurons under hypotonic perturbation. Am. J. Physiol. 271, C1895-C1900.
53. Ito, T., Suzuki, A., and Stossel, T. P. (1992). Regulation of water flow by actin-binding protein-induced actin gelation. Biophys. J. 61, 1301-1305.
54. Jacobson, K., and Wojcieszyn, J. (1984). The translational mobility of substances within the cytoplasmic matrix. Proc. Natl. Acad. Sci. U.S.A. 81, 6747-6751.
55. Jaken, S. (1996). Protein kinase c isozymes and substrates. Curr. Opin. Cell Biol. 8, 168-173.
56. Janson, L. W., Ragsdale, G. K., and Luby-Phelps, K. (1996). Mechanism and size cutoff for steric exclusion from actin-rich cytoplasmic domains. Biophys. J. 71, 1228-1234.
57. Johansen, K. M. (1996). Dynamic remodeling of nuclear architecture during the cell cycle. J. Cell. Biochem. 60, 289-296.
58. Jones, J. D., Ragsdale, G. K., Rozelle, A., Yin, H. L., and Luby-Phelps, K. (1998). Diffusion of vesicle-sized particles in living cells is restricted by intermediate filaments. Mol. Biol. Cell 8, 174a.
59. Kao, H. P., Abney, J. R., and Verkman, A. S. (1993). Determinants of the translational mobility of a small solute in cell cytoplasm. J. Cell Biol. 120, 175-184.
60. Kellermayer, M., Ludany, A., Miseta, A., Koszegi, T., Berta, G., Bogner, P., Hazlewood, C. F., Cameron, I. L., and Wheatley, D. N. (1994). Release of potassium, lipids, and proteins from nonionic detergent treated chicken red blood cells. J. Cell. Physiol. 159, 197-204.
61. Kempner, E. S., and Miller, J. H. (1968a). The molecular biology of Euglena gracilis. IV. Cellular stratification by centrifuging. Exp. Cell Res. 51, 141-149.
62. Kempner, E. S., and Miller, J. H. (1968b). The molecular biology of Euglena gracilis. V. Enzyme localization. Exp. Cell Res. 51, 150-156.
63. Kertesz, J. (1981). Percolation of holes between overlapping spheres: Monte Carlo calculation of the critical volume fraction. J. Phys. (Paris) 42, L393-L395.
64. Kislauskis, E. H., and Singer, R. H. (1991). Characterization of sequences responsible for peripheral localization of β-actin mRNA. J. Cell Biol. 115, 158a.
65. Kislauskis, E. H., Zhu, X., and Singer, R. H. (1994). Sequences responsible for intracellular localization of β-actin messenger RNA also affect cell phenotype. J. Cell Biol. 127, (2) 441-451.
66. Knowles, R. B., Sabry, J. H., Martone, M. E., Deerinck, T. J., Ellisman, M. H., Bassell, G. J., and Kosik, K. S. (1996). Translocation of RNA granules in living neurons. J. Neurosci. 16, 7812-7820.
67. Knull, H., and Minton, A. P. (1996). Structure within eukaryotic cytoplasm and its relationship to glycolytic metabolism. Cell Biochem. Funct. 14, 237-248.
68. Kreis, T. E., Geiger, B., and Schlessinger, J. (1982). Mobility of microinjected rhodamine actin within living chicken gizzard cells determined by fluorescence photobleaching recovery. Cell (Cambridge, Mass.) 29, 835-845.
69. Lai, Y. K., Lee, W. C., and Chen, K. D. (1993). Vimentin serves as a phosphate sink during the apparent activation of protein kinases by okadaic acid in mammalian cells. J. Cell. Biochem. 53, 161-168.
70. Lanni, F., Waggoner, A. S., and Taylor, D. L. (1985). Structural organization of interphase 3T3 fibroblasts studied by total internal reflection fluorescence microscopy. J. Cell Biol. 100, 1091-1102.
71. L'Ecuyer, T. J., Tompach, P. C., Morris, E., and Fulton, A. B. (1995). Transdifferentiation of chicken embryonic cells into muscle cells by the 3′ untranslated region of muscle tropomyosin. Proc. Natl. Acad. Sci. U.S.A. 92, 7520-7524.
72. Lepock, J. R., Cheng, K.-H., Campbell, S. D., and Kruuv, J. (1983). Rotational diffusion of tempone in the cytoplasm of chinese hamster lung cells. Biophys. J. 44, 405-412.
73. Lim, L., Manser, E., Leung, T., and Hall, C. (1996). Regulation of phosphorylation pathways by p21 GTPases. The p21 Ras-related Rho subfamily and its role in phosphorylation signalling pathways. Eur. J. Biochem. 242, 171-185.
74. Lindmo, T., and Steen, H. B. (1977). Flow cytometric measurement of the polarization of fluorescence from intracellular fluorescein in mammalian cells. Biophys. J. 18, 173-187.
75. Ling, G. N. (1988). A physical theory of the living state: Application to water and solute distribution. Scanning Microsc. 2, 899-913.
76. Ling, G. N., Niu, Z., and Ochsenfeld, M. (1993). Predictions of polarized multilayer theory of solute distribution confirmed from a study of the equilibrium distribution in frog muscle of twenty-one nonelectrolytes including five cryoprotectants. Physiol. Chem. Phys. Med. NMR 25, 177-208.
77. Livingston, D. J., LaMar, G. N., and Brown, W. D. (1983). Myoglobin diffusion in bovine heart muscle. Science 220, 71-73.
78. Luby-Phelps, K., and Taylor, D. L. (1988). Subcellular compartmentalization by local differentiation of cytoplasmic structure. Cell Motil. Cytoskel. 10, 28-37.
79. Luby-Phelps, K., Lanni, F., and Taylor, D. L. (1985). Behavior of a fluorescent analogue of calmodulin in living 3T3 cells. J. Cell Biol. 101, 1245-1256.
80. Luby-Phelps, K., Taylor, D. L., and Lanni, F. (1986). Probing the structure of cytoplasm. J. Cell Biol. 102, 2015-2022.
81. Luby-Phelps, K., Castle, P. E., Taylor, D. L., and Lanni, F. (1987). Hindered diffusion of inert tracer particles in the cytoplasm of mouse 3T3 cells. Proc. Natl. Acad. Sci. U.S.A. 84, 4910-4913.
82. Luby-Phelps, K., Mujumdar, S., Mujumdar, R., Ernst, L., Galbraith, W., and Waggoner, A. (1993). A novel fluorescence ratiometric method confirms the low solvent viscosity of the cytoplasm. Biophys. J. 65, 236-242.
83. 2+-regulated dynamic compartmentalization of calmodulin in living smooth muscle cells. J. Biol. Chem. 270, 21532-21538.
84. Maccioni, R. B., and Cambiazo, V. (1995). Role of microtubule-associated proteins in the control of microtubule assembly. Physiol. Rev. 75, 835-864.
85. Masters, C. (1992). Microenvironmental factors and the binding of glycolytic enzymes to contractile filaments. Int. J. Biochem. 24, 405-410.
86. Mastro, A. M., Babich, M. A., Taylor, W. D., and Keith, A. D. (1984). Diffusion of a small molecule in the cytoplasm of mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 81, 3414-3418.
87. Mejean, C., Pons, F., Benyamin, Y., and Roustan, C. (1989). Antigenic probes locate binding sites for the glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase, aldolase and phosphofructokinase on the actin monomer in microfilaments. Biochem. J. 264, 671-677.
88. Minaschek, G., Groschel-Stewart, U., Blum, S., and Bereiter-Hahn, J. (1992). Microcompartmentation of glycolytic enzymes in cultured cells. Eur. J. Cell Biol. 58, 481-428.
89. Minton, A. P. (1997). Influence of excluded volume upon macromolecular structure and associations in 'crowded' media. Curr. Opin. Biotechnol. 8, 65-69.
90. Mochly-Rosen, D., and Gordon, A. S. (1998). Anchoring proteins for protein kinase C-A means for isozyme selectivity. FASEB J. 12, 35-42.
91. Mon, H., Hiromoto, N., Nakahara, M., and Shiraishi, T. (1982). Stereological analysis of Leydig cell ultrastructure in aged humans. J. Clin. Endocrinol. Metab. 55, 634-641.
92. O'Reilly, G., and Clarke, F. (1993). Identification of an actin binding region in aldolase. FEBS Lett. 321, 69-72.
93. Ormo, M., Cubitt, A. B., Kallio, K., Gross, L. A., Tsien, R. Y., and Remington, S. J. (1996). Crystal structure of the aequorea victoria green fluorescent protein. Science 273, 1392-1395.
94. Ovádi, J., and Srere, P. A. (1996). Metabolic consequences of enzyme interactions. Cell Biochem. Fund. 14, 249-258.
95. Pagliaro, L., and Taylor, D. L. (1988). Aldolase exists in both the fluid and solid phases of cytoplasm. J. Cell Biol. 107, 981-991.
96. Pagliaro, L., Kerr, K., and Taylor, D. L. (1989). Enolase exists in the fluid phase of cytoplasm in 3T3 cells. J. Cell Sci. 94, 333-342.
97. Paine, P. L. (1984). Diffusive and nondiffusive proteins in vivo. J. Cell Biol. 99, 188s-195s.
98. Parsegian, V. A., Rand, R. P., and Rau, D. C. (1995). Macromolecules and water: Probing with osmotic stress. In "Methods in Enzymology," (M. L. Johnson and G. K. Ackers, eds.), Vol. 259, pp. 43-94. Academic Press, San Diego, CA.
99. Pawson, T., and Scott, J. D. (1997). Signaling through scaffold, anchoring, and adaptor proteins. Science 278, 2075-2080.
100. Pereira, G., and Schiebel, E. (1997). Centrosome-microtubule nucleation. J. Cell Sci. 110, 295-300.
101. Periasamy, N., Armijo, M., and Verkman, A. S. (1991). Picosecond rotation of small polar fluorophores in the cytosol of sea urchin eggs. Biochemistry 30, 11836-11841.
102. Periasamy, N., Kao, H. P., Fushimi, K., and Verkman, A. S. (1992). Organic osmolytes increase cytoplasmic viscosity in kidney cells. Am. J. Physiol. 263, C901-C907.
103. Popov, S., and Poo, M. M. (1992). Diffusional transport of macromolecules in developing nerve processes. J. Neurosci. 12, 77-85.
104. Porter, K. R. (1984). The cytomatrix: a short history of its study. J. Cell Biol. 99, 3s-12s.
105. Provance, D. W., MacDowall, A., Marko, M., and Luby-Phelps, K. (1993). Cytoarchitecture of size-excluding compartments in living cells. J. Cell Sci. 106, 565-578.
106. Reitz, F. B., and Pagliaro, L. (1997). Does regulatory protein play a role in glucokinase localization? Hormone & Metabolic Research. 29, 317-321.
107. Rhoads, A. R., and Friedberg, F. (1997). Sequence motifs for calmodulin recognition. FASEB J. 11, 331-340.
108. Rorschach, H. E., Chang, D. C., Hazlewood, C. F., and Nichols, B. L. (1973). The diffusion of water in striated muscle. Ann. N. Y. Acad. Sci. 204, 445-452.
109. Rorschach, H. E., Lin, C., and Hazlewood, C. F. (1991). Diffusion of water in biological tissues. Scanning Microsc., Suppl. 5, S9-S10.
110. Salmon, E. D., Saxton, W. M., Leslie, R. J., Karow, M. L., and McIntosh, J. R. (1984). Diffusion coefficient of fluorescein-labeled tubulin in the cytoplasm of embryonic cells of a sea urchin: Video image analysis of fluorescence redistribution after photobleaching. J. Cell Biol. 99, 2157-2164.
111. Saxton, M. J. (1995). Single-particle tracking: effects of corrals. Biophys. J. 69, 389-398.
112. Schliwa, M., and van Blerkom, J. (1981). Structural Interaction of cytoskeletal components. J. Cell Biol. 90, 222-235.
113. Seksek, O., Biwersi, J., and Verkman, A. S. (1997). Translational diffusion of macromoleculesized solutes in cytoplasm and nucleus. J. Cell Biol. 138, 131-142.
114. Simson, R., Sheets, E. D., and Jacobson, K. (1995). Detection of temporary lateral confinement of membrane proteins using single-particle tracking analysis. Biophys. J. 69, 989-993.
115. Singer, R. H., Langevin, G. L., and Lawrence, J. B. (1989). Ultrastructural visualization of cytoskeletal mRNAs and their associated proteins using double-label in situ hybridization. J. Cell Biol 108, 2343-2353.
116. Small, J. V., Isenberg, G., and Celis, J. E. (1978). Polarity of actin at the leading edge of cultured cells. Nature (London) 272, 638-639.
117. Srere, P. A. (1967). Enzyme concentrations in tissues. Science 158, 936-937.
118. Srivastava, A., and Krishnamoorthy, G. (1997). Cell type and spatial location dependence of cytoplasmic viscosity measured by time-resolved fluorescence microscopy. Arch. Biochem. Biophys. 340, 159-167.
119. Steyer, J. A., Horstmann, H., and Almers, W. (1997). Transport, docking and exocytosis of single secretory granules in live chromaffin cells. Nature (London) 388, 474-478.
120. Suprenant, K. A. (1993). Microtubules, ribosomes, and RNA: Evidence for cytoplasmic localization and translational regulation. Cell Motil. Cytoskel. 25, 1-9.
121. Swaminathan, R., Bicknese, S., Periasamy, N., and Verkman, A. S. (1996). Cytoplasmic viscosity near the cell plasma membrane-translational diffusion of a small fluorescent solute measured by total internal reflection-fluorescence photobleaching recovery. Biophys. J. 71, 1140-1151.
122. Swaminathan, R., Hoang, C. P., and Verkman, A. S. (1997). Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: Cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion. Biophys. J. 72, 1900-1907.
123. Tang, J. X., and Janmey, P. A. (1996). The polyelectrolyte nature of F-actin and the mechanism of actin bundle formation. J. Biol. Chem. 271, 8556-8563.
124. 2+ sensitivity of light chain phosphorylation within smooth muscle cells. J. Biol. Chem. 269, 9912-9920.
125. Tapon, N., and Hall, A. (1997). Rho, Rac and Cdc42 GTPases regulate the organization of the actin cytoskeleton. Curr. Opin. Cell Biol. 9, 86-92.
126. Trantham, E. C., Rorschach, H. E., Clegg, J. S., Hazlewood, C. F., Nicklow, R. M., and Wakabayashi, N. (1984). Diffusive properties of water in artemia cysts as determined from quasi-elastic neutron scattering spectra. Biophys. J. 45, 927-938.
127. Van Steveninck, J., Paardekooper, M., Dubbelman, T. M. A. R., and Ben-Hur, E. (1991). Anomalous properties of water in macromolecular gels. Biochim. Biophys. Acta 1115, 96-100.
128. Wang, J., Morris, A. J., Tolan, D. R., and Pagliaro, L. (1996). The molecular nature of the F-actin binding activity of aldolase revealed with site-directed mutants. J. Biol. Chem. 271, 6861-6865.
129. Wang, Y., Lanni, F., McNeil, P. L., Ware, B. R., and Taylor, D. L. (1982). Mobility of cytoplasmic and membrane-associated actin in living cells. Proc. Natl. Acad. Sci. U.S.A. 79, 4660-4664.
130. Waterman-Storer, C. M., and Salmon, E. D. (1997). Microtubule dynamics: Treadmilling comes around again. Curr. Biol. 7, R369-R372.
131. Webster, R. E., Henderson, D., Osborn, M., and Weber, K. (1978). Three-dimensional electron microscopical visualization of the cytoskeleton of animal cells: Immunoferritin identification of actin- and tubulin-containing structures. Proc. Natl. Acad. Sci. U.S.A. 75, 5511-5515.
132. Wheatley, D. N. (1998). Diffusion theory, the cell and the synapse. BioSystems 45, 151-163.
133. Wheatley, D. N., and Malone, P. C. (1993). Heat conductance, diffusion theory and intracellular metabolic regulation. Biol. Cell 79, 1-5.
134. Wiggins, P. M. (1996). High and low density water and resting, active and transformed cells. Cell Biol. Int. 20, 429-435.
135. Wiggins, P. M., van Ryn, R. T., and Ormrod, D. G. C. (1991). Donnan membrane equilibrium is not directly applicable to distributions of ions and water in gels or cells. Biophys. J. 60, 8-14.
136. Williams, S. P., Haggle, P. M., and Brindle, K. M. (1997). F-19 nmr measurements of the rotational mobility of proteins in vivo. Biophys. J. 72, 490-498.
137. Wilson, I. A., Brindle, K. M., and Fulton, A. M. (1995). Differential localization of the mRNA of the M and B isoforms of creatine kinase in myoblasts. Biochem. J. 308, 599-605.
138. Wojcieszyn, J. W., Schlegel, R. A., Wu, E., and Jacobson, K. A. (1981). Diffusion of injected macromolecules within the cytoplasm of living cells. Proc. Natl. Acad. Sci. U.S.A. 78, 4407-4410.
139. Wojtas, K., Slepecky, N., von Kalm, L., and Sullivan, D. (1997). Flight muscle function in Drosophila requires colocalization of glycolytic enzymes. Mol. Biol. Cell. 8, 1665-1675.
140. Wolosewick, J. J., and Porter, K. R. (1979). Microtrabecular lattice of the cytoplasmic ground substance. J. Cell Biol. 82, 114-139.
141. Yang, F., Moss, L. G., and Phillips, G. N. (1996). The molecular structure of green fluorescent protein. Nat. Biotechnol. 14, 1246-1251.
142. Yguerabide, J., Schmidt, J. A., and Yguerabide, E. E. (1982). Lateral mobility in membranes as detected by fluorescence recovery after photobleaching. Biophys. J. 39, 69-75.
143. Yokoe, E., and Meyer, T. (1996). Spatial dynamics of gfp-tagged proteins investigated by local fluorescence enhancement. Nat. Biotechnol. 14, 1252-1256.
144. Zalokar, M. (1960). Cytochemistry of centrifuged hyphae of Neurospora. Exp. Cell Res. 19, 114-132.
145. Zimmermann, B., Somlyo, A. V., Ellis-Davies, G. C., Kaplan, J. H., and Somlyo, A. P. (1995). Kinetics of prephosphorylation reactions and myosin light chain phosphorylation in smooth muscle. Flash photolysis studies with caged calcium and caged ATP. J. Biol. Chem. 270, 23966-23974.