Translational diffusion of macromolecule-sized solutes in cytoplasm and nucleus

Journal of Cell Biology

Volumn 138, Issue 1, 1997, Pages 131-142

  Seksek, Olivier ^a,b   Biwersi, Joachim ^a   Verkman, A S ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; CELL NUCLEUS; CONTROLLED STUDY; CYTOPLASM; DIFFUSION; DOG; INTRACELLULAR TRANSPORT; KIDNEY CELL; MACROMOLECULE; NONHUMAN; PRIORITY JOURNAL; TRANSPORT KINETICS;

3T3 CELLS; ANIMALS; CELL LINE; CELL NUCLEUS; CYTOPLASM; DEXTRANS; DIFFUSION; DOGS; FICOLL; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENCE; MACROMOLECULAR SUBSTANCES; MICE; SOLUTIONS; TEMPERATURE;

ANIMALIA; CANIS FAMILIARIS;

EID: 0030742748     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.138.1.131     Document Type: Article

References (44)

1. Almeida, P.F.F., and W.L.C. Vaz. 1995. Lateral diffusion in membranes. In Handbook of Biological Physics. R. Lipowsky and E. Sackmann, editors. Elsevier Science Publishing Co., Inc., New York. 305-345.
2. Alexander, S.P., and C.L. Rieder. 1991. Chromosome motion during attachment to the vertebrate spindle: initial saltatory-like behavior of chromosomes and quantitative analysis of force production by nascent kinetochore fibers. J. Cell Biol. 113:805-815.
3. Axelrod, D., D.E. Koppel, J. Schlessinger, E. Elson, and W.W. Webb. 1976. Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys. J. 16:1055-1069.
4. Berezney, R. 1991. The nuclear matrix: a heuristic model for investigating genomic organization and function in the cell nucleus. J. Cell. Biochem. 47: 109-123.
5. Bicknese, S., N. Periasamy, S.B. Shohet, and A.S. Verkman. 1993. Cytoplasmic viscosity near the cell plasma membrane, measurement by evanescent field frequency-domain microfluorimetry. Biophys. J. 165:1272-1282.
6. Capco, D.G., K.M. Wan, and S. Penman. 1982. The nuclear matrix: three-dimensional architecture and protein composition. Cell. 29:847-858.
7. Clegg, J.S. 1984. Properties and metabolism of the aqueous cytoplasm and its boundaries. Am. J. Physiol. 246:R133-R151.
8. Cook, P.R. 1991. The nucleoskeleton and the topology of replication. Cell. 66: 627-635.
9. Farinas, J., V. Simenak, and A.S. Verkman. 1995. Cell volume measured in adherent cells by total internal reflection microfluorimetry: application to permeability in cells transacted with water channel homologs. Biophys. J. 68: 1613-1620.
10. Feder, T.J., I. Brust-Mascher, J.P. Slattery, B. Baird, and W.W. Webb. 1996. Constrained diffusion or immobile fraction on cell surfaces: a new interpretation. Biophys. J. 70:2367-2373.
11. Fey, E.G., G. Krochmalnic, and S. Penman. 1986. The nonchromatin substructures of the nucleus: the ribonucleoprotein (RNP)-containing and RNP-depleted matrices analyzed by sequential fractionation and resinless section electron microscopy. J. Cell Biol. 102:1654-1665.
12. Fulton, A.B. 1982. How crowded is the cytoplasm? Cell. 30:345-347.
13. Furakawa, R., J.L. Arauz-Lara, and B.R. Ware. 1991. Self-diffusion and probe diffusion in dilute and semidilute aqueous solutions of dextran. Macromolecules. 24:599-605.
14. Fushimi, K., and A.S. Verkman. 1991. Low viscosity in the aqueous domain of cytoplasm measured by picosecond polarization microscopy. J. Cell Biol. 112:719-725.
15. Goodsell, D.S. 1991. Inside a living cell. TIBS. 16:203-206.
16. He, D.C., J.A Nickerson, and S. Penman. 1990. Core filaments of the nuclear matrix. J. Cell Biol. 110:569-580.
17. Hou, L., F. Lanni, and K. Luby-Phelps. 1990. Tracer diffusion of F-actin and Ficoll mixtures: toward a model for cytoplasm. Biophys. J. 58:31-43.
18. Inman, J.K. 1975. Thymus-independent antigens: the preparation of covalent, hapten-Ficoll conjugates. J. Immunol. 114:704-709.
19. Jacobson, D.A. 1995. Nuclear organization: uniting replication foci, chromatin domains and chromosome structure. Bioessays. 17:587-591.
20. Kao, H.P., and A.S. Verkman. 1994. Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position. Biophys. J. 67:1291-1300.
21. Kao, H.P., and A.S. Verkman. 1996. Construction and performance of a FRAP instrument with microsecond time resolution. Biophys. Chem. 59:203-210.
22. Kao, H.P., J.R. Abney, and A.S. Verkman. 1993. Determinants of the translational diffusion of a small solute in cytoplasm. J. Cell Biol. 120:175-184.
23. Lang, I., M. Scholz, and R. Peters. 1986. Molecular mobility and nucleocytoplasmic flux in hepatoma cells. J. Cell Biol. 102:1183-1190.
24. Lopez, A., L. Dupou, A. Altibelli, J. Trotard, and J.F. Tocanne. 1988. Fluorescence recovery after photobleaching (FRAP) experiments under conditions of uniform disk illumination. Biophys. J. 53:963-970.
25. Luby-Phelps, K. 1994. Physical properties of cytoplasm. Curr. Opin. Cell Biol. 6:3-9.
26. Luby-Phelps, K., D.L. Taylor, and F. Lanni. 1986. Probing the structure of cytoplasm. J. Cell Biol. 102:2015-2022.
27. Luby-Phelps, K., P.E. Castle, D.L. Taylor, and F. Lanni. 1987. Hindered diffusion of inert tracer particles in the cytoplasm of mouse 3T3 fibroblasts. Proc. Natl. Acad. Sci. USA. 84:4910-4913.
28. Luby-Phelps, K., S. Mujundar, R. Mujundar, L. Ernst, W. Galbraith, and A. Waggoner. 1993. A novel fluorescence ratiometric method confirms the low solvent viscosity of the cytoplasm. Biophys. J. 65:236-242.
29. Mastro, A.M., and A.D. Keith. 1984. Diffusion in the aqueous compartment. J. Cell Biol. 99:180s-187s.
30. McNeil, P.L., and E. Warder. 1987. Glass beads load macromolecules into living cells. J. Cell Sci. 88:669-678.
31. Nagle, J.F. 1992. Long tail kinetics in biophysics? Biophys. J. 63:366-370.
32. Periasamy, N., S. Bicknese, and A.S. Verkman. 1996. Reversible photobleaching of fluorescein conjugates in air-saturated viscous solutions: molecular tryptophan as a triplet state quencher. Photochem. Photobiol. 63:265-271.
33. Phillies, G.D.J. 1987. Dynamics of polymers in concentrated solutions: the universal scaling equation derived. Macromolecules. 20:558-564.
34. Phillies, G.D.J. 1989. The hydrodynamic scaling method for polymer self-diffusion. J. Phys. Chem. 93:5029-5039.
35. Porter, K.R. 1984. The cytomatrix: a short history of its study. J. Cell Biol. 99: 3s-12s.
36. Provance, D.W., A. McDowall, M. Marko, and K. Luby-Phelps. 1993. Cytoarchitecture of size-excluding compartments in living cells. J. Cell Sci. 106:565-577.
37. Raska, I., M. Dundr, and K. Koberna. 1992. Structure-function subcompartments of the mammalian cell nucleus as revealed by the electron microscopy affinity cytochemistry. Cell Biol. Int. Rep. 16:771-789.
38. Saxton, M.J. 1993. Lateral diffusion in an archipelago. Dependence on tracer size. Biophys. J. 64:1053-1062.
39. Saxton, M.J. 1994. Anomalous diffusion due to obstacles: a Monte Carlo study. Biophys. J. 66:394-401.
40. Seksek, O., J. Biwersi, and A.S. Verkman. 1995. Direct measurement of trans-Golgi pH in living cells and regulation by second messengers. J. Biol. Chem. 270:4967-4970.
41. Swaminathan, R., S. Bicknese, N. Periasamy, and A.S. Verkman. 1996. Cytoplasmic viscosity near the cell plasma membrane: translation of BCECF measured by total internal reflection-fluorescence photobleaching recovery. Biophys. J. 71:1140-1151.
42. Swaminathan, R., C.P. Hoang, and A.S. Verkman. 1997. Photochemical properties of green fluorescent protein GFP-S65T in solution and transfected CHO cells: analysis of cytoplasmic viscosity by GFP translational and rotational diffusion. Biophys. J. 72:1900-1907.
43. Verkman, A.S. 1991. New microfluorimetry approaches to examine cell dynamics. Comments Mol. Cell. Biophys. 7:173-187.
44. Welch, G.R., and J.S. Easterby. 1994. Metabolic channeling versus free diffusion: transition-time analysis. TIBS. 19:193-197.