Skeletor, a novel chromosomal protein that redistributes during mitosis provides evidence for the formation of a spindle matrix

Journal of Cell Biology

Volumn 151, Issue 7, 2000, Pages 1401-1411

  Walker, Diana L ^a   Wang, Dong ^a   Jin, Ye ^a   Rath, Uttama ^a   Wang, Yanming ^a   Johansen, Jørgen ^a   Johansen, Kristen M ^a  

^a IOWA STATE UNIVERSITY   (United States)

Author keywords

Chromosomes; Drosophila; Microtubules; Mitosis; Spindle matrix

Indexed keywords

CHROMOSOME PROTEIN;

ANAPHASE; ANTIBODY LABELING; ARTICLE; CHROMOSOME SEGREGATION; DROSOPHILA; EMBRYO DEVELOPMENT; EXPRESSED SEQUENCE TAG; INTERPHASE; METAPHASE; MICROTUBULE; MICROTUBULE ASSEMBLY; MITOSIS; MITOSIS SPINDLE; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROPHASE; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; SEQUENCE ANALYSIS;

ACTIVE TRANSPORT, CELL NUCLEUS; AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES; BASE SEQUENCE; CELL NUCLEUS; CHROMOSOMAL PROTEINS, NON-HISTONE; CLONING, MOLECULAR; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; IMMUNOHISTOCHEMISTRY; MITOSIS; MITOTIC SPINDLE APPARATUS; MOLECULAR SEQUENCE DATA; NOCODAZOLE; NUCLEAR MATRIX-ASSOCIATED PROTEINS; OPEN READING FRAMES; RNA, MESSENGER; SEQUENCE ALIGNMENT; TEMPERATURE;

EID: 0034739843     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.151.7.1401     Document Type: Article

References (43)

1. The genome sequence of Drosophila melanogaster
2. An efficient method for microinjection of mRNA into Drosophila embryos
3. Autoantibodies to a novel cell cycle-regulated protein that accumulates in the nuclear matrix during S phase and is localized in the kinetochores and spindle midzone during mitosis
4. Primary structure of NuMA, an intranuclear protein that defines a novel pathway for segregation of proteins at mitosis
5. The inner centromere protein (INCENP) antigens: Movement from inner centromere to midbody during mitosis
6. NuMA is a component of an insoluble matrix at mitotic spindle poles
7. Analysis of the distribution of the IN-CENPs throughout mitosis reveals the existence of a pathway of structural change in the chromosomes during metaphase, and the early events in cleavage furrow formation
8. Chromosomal proteins of Drosophila embryos
9. Ultraviolet microbeam irradiations of spindle fibres in crane-fly spermatocytes and newt epithelial cells: Resolution of previously conflicting observations
10. Microtubule organization during maturation of Xenopus oocytes: Assembly and rotation of the meiotic spindles
11. A microtubule-associated protein in Drosophila melanogaster: Identification, characterization, and isolation of coding sequences
12. Differential glycosylation of tractin and LeechCAM, two novel Ig superfamily members, regulates neurite extension and fascicle formation
13. JIL-1: A novel chromosomal tandem kinase implicated in transcriptional regulation in Drosophila
14. Dynamic remodeling of nuclear architecture during the cell cycle
15. Remodeling of nuclear architecture during the cell cycle in Drosophila embryos
16. Chromatin structure and nuclear remodeling
17. Behavior of microtubules and actin filaments in living Drosophila embryos
18. CP60: A microtubule-associated protein that is localized to the centrosome in a cell cycle-specific manner
19. Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles
20. Human specific nuclear protein that associates with the polar region of the mitotic apparatus: Distribution in a human/hamster hybrid cell
21. Molecular analysis of the INCENPs (inner centromere proteins): Separate domains are required for association with microtubules during interphase and with the central spindle during anaphase
22. A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly
23. Polewards microtubule flux in the mitotic spindle: Evidence from photoactivation of fluorescence
24. Localization of antibody determinants to whole Drosophila embryos
25. The cell cycle-dependent localization of the CP190 centrosomal protein is determined by the coordinate action of two separable domains
26. Two proteins that cycle asynchronously between centrosomes and nuclear structures: Drosophila CP60 and CP90
27. Time-resolved, in vivo studies of mitotic spindle formation and nuclear lamina breakdown in Drosophila early embryos
28. An antigen located in the kinetochore region in metaphase and on polar microtubule ends in the mid-body region in anaphase, characterized using a monoclonal antibody
29. Traction fiber: Toward a "tensegral" model of the spindle
30. Using neural networks for prediction of the subcellular location of proteins
31. Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: Identification of a class of bipartite nuclear targeting sequence
32. Poleward microtubule flux in mitotic spindles assembled in vitro
33. Microtubule flux in mitosis is independent of chromosomes, centrosomes, and antiparallel microtubules
34. Meiotic spindle assembly in Drosophila females: Behavior of nonexchange chromosomes and the effects of mutations in the nod kinesin-like protein
35. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
36. The nuclear-mitotic apparatus protein is important in the establishment and maintenance of the bipolar mitotic spindle apparatus
37. CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase
38. Chromosomes initiate spindle assembly upon experimental dissolution of the nuclear envelope in grasshopper spermatocytes
39. The impact of chromosomes and centrosomes on spindle assembly as observed in living cells
40. In vivo binding pattern of a trans-regulator of homoeotic genes in Drosophila melanogaster