Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis

Journal of Cell Biology

Volumn 132, Issue 4, 1996, Pages 617-633

  Echeverri, Christophe J ^a   Paschal, Bryce M ^b   Vaughan, Kevin T ^a   Vallee, Richard B ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; COMPLEMENTARY DNA; DYNACTIN; DYNEIN ADENOSINE TRIPHOSPHATASE; PROTEIN; PROTEIN SUBUNIT; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CATTLE; CENTROMERE; CERCOPITHECIDAE; CHROMOSOME; IMMUNOBLOTTING; IMMUNOFLUORESCENCE MICROSCOPY; MALE; MITOSIS; MITOSIS SPINDLE; NONHUMAN; NORTHERN BLOTTING; OPEN READING FRAME; PRIORITY JOURNAL; RAT;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CATTLE; CELL LINE; CHROMOSOMES; CLONING, MOLECULAR; DNA, COMPLEMENTARY; DYNEIN ATPASE; HUMANS; MALE; MICROTUBULE PROTEINS; MICROTUBULE-ASSOCIATED PROTEINS; MITOSIS; MOLECULAR SEQUENCE DATA; RATS; RATS, SPRAGUE-DAWLEY; SEQUENCE ANALYSIS;

ANIMALIA; BOS TAURUS; CERCOPITHECIDAE; VERTEBRATA;

EID: 0029913484     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.132.4.617     Document Type: Article

References (67)

1. Adams, M.D., M. Bento Soares, A.R. Kerlavage, C. Fields, and J.C. Venter. 1993. Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nature Genet. 4:373-380.
2. Aebersold, R. 1989. In A Practical Guide to Protein and Peptide Purification for Microsequencing. P.R. Matsudaira, editor. Academic Press, San Diego, CA, 71-38
3. Altschul, S.F., W. Gish, W. Miller, E.W. Myers, and D.J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
4. Aniento, F., N. Emans, G. Griffiths, and J. Gruenberg. 1993. Cytoplasmic dynein-dependent vesicular transport from early to late endosomes. J. Cell Biol. 123:1373-1387.
5. Bernat, R.L., G.G. Borisy, N.F. Rothfield, and W.C. Earnshaw. 1990. Injection of anticentromere antibodies in interphase disrupts events required for chromosome movement at mitosis. J. Cell Biol. 111:1519-1533.
6. Bernat, R.L., M.R. Delannoy, N.F. Rothfield, and W.C. Earnshaw. 1991. Disruption of centromere assembly during interphase inhibits kinetochore morphogenesis and function in mitosis. Cell. 66:1229-1238
7. Clark, S W., and D.I. Meyer. 1992. Centractin is an actin homologue associated with the centrosome. Nature (Lond.). 359:246-250.
8. Clark, S.W., and D I. Meyer 1994. ACT3: a putative centractin homologue in S. cerevisiae is required for proper orientation of the mitotic spindle. J. Cell Biol. 127:129-138.
9. Clark, S.W., O. Staub, I.R. Clark, E L F. Holzbaur, B.M. Paschal, R.B. Vallee, and D.I. Meyer. 1994. β-Centractin: characterization and distribution of a new member of the centractin family of actin-related proteins. Mol. Biol. Cell. 5:1301-1310.
10. Collins, C. A., and R.B. Vallee. 1989. Preparation of microtubules from rat liver and testes:cytoplasmic dynein is a major microtubule-associated protein. Cell Motil. Cytoskeleton. 14 491-500.
11. Corthésy-Theulaz, I., A. Pauloin, and S.R. Pfeffer. 1992. Cytoplasmic dynein participates in the centrosomal localization of the Golgi complex. J. Cell Biol. 118:1333-1345.
12. Desai, A , and T.J. Mitchison. 1995. A new role for motor proteins as couplers to depolymerizing microtubules. J. Cell Biol. 128:1-4.
13. Dillman, J.F., and K.K. Pfister. 1994. Differential phosphorylation in vivo of cytoplasmic dynein associated with anterogradely moving organelles. J. Cell Biol. 127:1671-1681.
14. Eshel, D., L.A. Urrestarauzu, S. Vissers, J.C. Jauniaux, J C. van Vliet-Reedijk, R.J. Planta, and I.R. Gibbons. 1993. Cytoplasmic dynein is required for normal nuclear segregation in yeast. Proc. Natl. Acad. Sci. USA. 90 11172-11176.
15. Evan, G.I., G.K. Lewis, G. Ramsay, and J.M. Bishop. 1985. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell. Biol 5.3610-3616.
16. Fath, K.R., G.M. Trimbur, and D.R. Burgess. 1994. Molecular motors are differentially distributed on Golgi membranes from polarized epithelial cells. J. Cell Biol 126:661-675.
17. Gill, S.R T., T.A. Schroer, I Szilak, E.R. Steuer, M.P. Sheetz, and D.W. Cleveland. 1991. Dynactin, a conserved, ubiquitously expressed component of an activator of vesicle motility mediated by cytoplasmic dynein. J. Cell Biol. 115:1639-1650.
18. Gorbsky, G.J., P.J. Sammak, and G.G. Borisy. 1987. Chromosomes move pole-ward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends J Cell Biol. 104:9-18.
19. Harte, P.J., and D.R. Kankel. 1982. Genetic analysis of mutations at the Glued locus and interacting loci in Drosophila melanogaster. Genetics. 101:477-501.
20. Hayden, J H., S.S. Bowser, and C.L. Rieder. 1990. Kinetochores capture astral microtubules during chromosome attachment to the mitotic spindle direct visualization in live newt lung cells. J. Cell Biol. 111:1039-1045
21. Henikoff, S., and J.G. Henikoff. 1994. Protein family classification based on searching a database of blocks. Genomics. 19:97-107.
22. Holzbaur, E L.F., J.A. Hammarback, B.M. Paschal, N.G. Kravit, K K. Pfister, and R.B Vallee. 1991. Homology of a 150 kD cytoplasmic dynein-associated polypeptide with the Drosophila gene Glued Nature (Lond.). 351:579-583.
23. Holzbaur, E.L.F., and R.B. Vallee. 1994. Dyneins: molecular structure and cellular function. Annu. Rev. Cell Biol 10:339-372.
24. Hyman, A.A., and T J. Mitchison. 1991. Two different microtubule-based motor activities with opposite polarities in kinetochores. Nature (Lond). 351: 206-211.
25. Jacobs, K.A., R. Rudersdorf, S.D. Neill, J.P. Dougherty, E L. Brown, and E.F Fritsch. 1988. The thermal stability of oligonucleotide duplexes is sequence independent in tetraalkylammonium salt solutions: application to identifying recombinant DNA clones. Nucleic Acids Res. 16:4637-4650.
26. Karki, S , and E.L.F. Holzbaur. 1995. Affinity chromatography demonstrates a direct binding between cytoplasmic dynein and ihe dynactin complex J Biol. Chem. 270:28806-28811.
27. King, S.M., and G.B. Witman 1990. Localization of an intermediate chain of outer arm dynein by immunoelectron microscopy. J. Biol. Chem 265:19807-19811.
28. Kozak, M. 1991. An analysis of vertebrate mRNA sequences: intimations of translational control. J. Cell Biol 115.887-903.
29. Lacey, M.L., and L.T. Haimo. 1992 Cytoplasmic dynein is a vesicle protein. J Biol Chem. 267:4793-4798
30. Lees-Miller, J.P., D.M. Helfman, and T.A. Schroer. 1992. A vertebrate actin-related protein is a component of a multi-subunit complex involved in microtubule-based vesicle motility. Nature (Lond.) 359:244-246.
31. Li, Y.Y., E. Yeh, T. Hays, and K. Bloom. 1993. Disruption of mitotic spindle orientation in a yeast dynein mutant. Proc. Natl. Acad. Sci. USA. 90.10096-100100
32. Lin, S.X., and C.A. Collins. 1992. Immunolocalization of cytoplasmic dynein to lysosomes in culture cells. J Cell Sci 101:125-137.
33. Lipman, D.J , and W.R Pearson. 1985. Rapid and sensitive protein similarity searches. Science (Wash. DC). 227. 1435-1441.
34. Lipman, D.J., and W.R. Pearson. 1988. Improved tools for biological sequence comparison. Proc. Natl Acad. Sci USA. 85:2444-2448.
35. Lombillo, V A., C. Nislow, T.J. Yen, V.I. Gelfand, and J.R. McIntosh. 1995 Antibodies to the kinesin motor domain and CENP-E inhibit microtubule depolymerization-dependent motion of chromosomes in vitro. J. Cell Biol. 128:107-115.
36. Lupas, A., M. Van Dyke, and J Stock 1991. Predicting coiled coils from protein sequences. Science (Wash. DC). 252:1162-1164.
37. McGrail, M., J. Gepner, A. Silvanovich, S. Ludmann, M. Serr, and T.S. Hays. 1995 Regulation of cytoplasmic dynein function in vivo by the Drosophila Glued complex. J. Cell Biol 131:411-425.
38. McMillan, J.N., and K. Tatchell. 1994. The JNM1 gene m the yeast Saccharomyces cerevisiae is required for nuclear migration and spindle orientation during the mitotic cell cycle. J. Cell Biol 125:143-158.
39. Glued component of the dynactin complex in developing and adult rat brain. J. Comp. Neurol. 357:15-24.
40. Mitehison, T.J., and M.W. Kirschner. 1985. Properties of the kinetochore in vitro. I. Microtubule nucleation and tubulin binding. J. Cell Biol. 101:755-765.
41. Mitchison, T.J., L. Evans, E. Schultz, and M.W. Kirschner. 1986. Sites of microtubule assembly and disassembly in the mitotic spindle. Cell. 45:515-527.
42. Muhua, L., T.S. Karpova, and J.A. Cooper. 1994. A yeast actin-related protein homologous to that found in vertebrate dynactin complex is important for spindle orientation and nuclear migration. Cell 78:669-679.
43. Nicklas, R.B., and D.F. Kubai 1985. Microtubules, chromosome movement, and reorientation after chromosomes are detached from the spindle by micromanipulation Chromosoma. 92:313-324.
44. Paschal, B.M., and R.B. Vallee. 1987. Retrograde transport by the microtubule associated protein MAP 1C. Nature (Lond.). 330:181-183.
45. Paschal, B.M., A. Mikami, K K Pfister, and R.B. Vallee. 1992. Homology of the 74-kD cytoplasmic dynein subunit with a flagellar dynein polypeptide suggests an intracellular targeting function. J. Cell Biol. 118:1133-1143.
46. Glued and a novel actin. J. Biol Chem. 268:15318-15323.
47. Pfarr, C.M., M. Coue, P.M. Grisson, T.S. Hays, M.E Porter, and J.R. McIntosh. 1990 Cytoplasmic dynein is localized to kinetochores during mitosis. Nature (Lond.). 345 263-265.
48. Pierre, P., J Scheel, J.E. Rickard, and T.E. Kreis. 1992. CLIP-170 links endocytic vesicles to microtubules. Cell 70.887-900
49. Plamann, M., P.F Minke, J.H. Tinsley, and K.S. Bruno. 1994. Cytoplasmic dynein and actin-related protein Arp-1 are required for normal nuclear distribution in filamentous fungi. J. Cell Biol 127:139-149.
50. Rieder, C.L. 1982. The formation, structure, and composition of the mammalian kinetochore and kinetochore fiber. Int. Rev Cytol. 79:1-58.
51. Rieder, C.L., and S.P. Alexander. 1990. Kinetochores are transported poleward along a single astral microtubule during chromosome attachment to the spindle in newt lung cells. J Cell Biol. 110:81-95.
52. Rieder, C.L., and E.D. Salmon. 1994. Motile kinetochores and polar ejection forces dictate chromosome position on the vertebrate mitotic spindle. J. Cell Biol. 124:223-233.
53. Saunders, W.S., D. Koshland, D. Eshel, I.R Gibbons, and M.A. Hoyt. 1995. Saccharomyces cerevisiae kinesin- and dynein-related proteins required for anaphase chromosome segregation. J. Cell Biol. 128.617-624.
54. Schafer, D A , S.R. Gill, J.A. Cooper, J.E. Heuser, and T A. Schroer. 1994. Ultrastructural analysis of the dynactin complex, an actin-related protein is a component of a filament that resembles F-actin. J. Cell Biol 126:403-412.
55. Schnapp, B.J., and T.S. Reese. 1989. Dynein is the motor for retrograde axonal transport of organelles. Proc. Natl. Acad. Sci. USA. 86.1548-1552.
56. Schroer, T.A., and M.P. Sheetz 1991. Two activators of microtubule-based vesicle transport. J. Cell Biol. 115:1309-1318.
57. Schroer, T A., E.R. Steuer, and M.P. Sheetz. 1989 Cytoplasmic dynein is a minus end-directed motor for membranous organelles. Cell. 56:937-946.
58. Simerly, C., R. Balczon, B.R. Brinkley, and G. Schatten. 1990. Microinjected kinetochore antibodies interfere with chromosome movement in meiotic and mitotic mouse oocytes. J. Cell Biol. 111:1491-1504.
59. Skibbens, R.V., V.P. Skeen, and E.D. Salmon. 1993. Directional instability of kinetochore motility during chromosome congression and segregation in mitotic newt lung cells: a push-pull mechanism. J. Cell Biol. 122:859-875.
60. Spurck, T.P., O.G. Stonington, J.A. Snyder, J.D. Pickett-Heaps, A. Bajer, and J. Mole-Bajer. 1990. UV microbeam irradiations of the mitotic spindle. II. Spindle fiber dynamics and force production. J. Cell Biol. 111:1505-1518.
61. Steuer, E.R., L. Wordeman, T.A. Schroer, and M.P. Sheetz. 1990. Localization of cytoplasmic dynein to mitotic spindles kinetochores Nature (Lond.). 345: 266-268.
62. Vaisberg, E.A., M.P. Koonce, and J.R. McIntosh. 1993. Cytoplasmic dynein plays a role in mammalian mitotic spindle formation. J. Cell Biol. 123:849-858
63. Glued. J. Cell Biol. 131:1507-1516.
64. Glued component of the dynactin complex binds to both microtubules and the actin-related protein centractin (Arp-1). Proc. Natl. Acad. Sci. USA. 92:1634-1638.
65. Xiang, X., S.M. Beckwith, and N.R. Morris. 1994. Cytoplasmic dynein is involved in nuclear migration in Aspergillus nidulans. Proc. Natl. Acad. Sci. USA 91:2100-2104.
66. Yen, T.J , D.A. Compton, D. Wise, R.P Zinkowski, B.R. Brinkley, W C. Earnshaw, and D.W. Cleveland. 1991. CENP-E, a novel centromere associated protein required for progression from metaphase to anaphase. EMBO (Eur. Mol. Biol. Organ.) J. 10:1245-1254.
67. Yen, T.J., G. Li, B T. Schaar, I. Szilak, and D W. Cleveland. 1992. CENP-E is a putative kinetochore motor that accumulates just before mitosis. Nature (Lond.) 359:536-539.