Regulation of Op18 during spindle assembly in Xenopus egg extracts

Journal of Cell Biology

Volumn 153, Issue 1, 2001, Pages 149-157

  Budde, Priya Prakash ^a   Kumagai, Akiko ^b   Dunphy, William G ^b   Heald, Rebecca ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Chromatin; Microtubule dynamics; P1x1; Phosphorylation; Spindle assembly

Indexed keywords

ONCOPROTEIN; ONCOPROTEIN 18; PROTEIN PLX1; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; CHROMATIN; CONTROLLED STUDY; EGG; ENZYME REGULATION; MICROTUBULE ASSEMBLY; MITOSIS; MITOSIS SPINDLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN PHOSPHORYLATION; PROTEIN STABILITY; XENOPUS;

ANIMALS; BINDING SITES; CELL CYCLE PROTEINS; CELL EXTRACTS; CHROMATIN; MICROTUBULE PROTEINS; MITOTIC SPINDLE APPARATUS; OVUM; PHOSPHOPROTEINS; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; STATHMIN; XENOPUS; XENOPUS PROTEINS;

AMPHIBIA; REPTILIA;

EID: 0035795412     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.153.1.149     Document Type: Article

References (68)

1. Spindle assembly and the art of regulating microtubule dynamics by MAPs and Stathmin/Op18
2. Mitotic chromatin regulates phosphorylation of Stathmin/Op18
3. GFP tagging reveals human Polo-like kinase 1 at the kinetochore/centromere region of mitotic chromosomes
4. Role of polo kinase and Midlp in determining the site of cell division in fission yeast
5. Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules
6. Cell-cycle-regulated phosphorylation of oncoprotein 18 on Ser16, Ser25 and Ser38
7. Drosophila polo kinase is required for cytokinesis
8. Accessory protein regulation of microtubule dynamics throughout the cell cycle
9. The Polo-related kinase Cdc5 activates and is destroyed by the mitotic cyclin destruction machinery in S. cerevisiae
10. The stathmin/tubulin interaction in vitro
11. Overexpression of stathmin in breast carcinomas points out to highly proliferative tumours
12. Microtubule polymerization dynamics
13. The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro
14. The Polo-like kinase P1x1 is required for M phase exit and destruction of mitotic regulators in Xenopus egg extracts
15. Phosphorylation regulates the microtubule-destabilizing activity of stathmin and its interaction with tubulin
16. A post-ribosomal supernatant from activated Xenopus eggs that displays post-translationally regulated oscillation of its cdc2+ mitotic kinase activity
17. Ectopic spindle assembly during maturation of Xenopus oocytes: Evidence for functional polarization of the oocyte cortex
18. The stathmin phosphoprotein family: Intracellular localization and effects on the microtubule network
19. The 4 ANG x-ray structure of a tubulinstathmin-like domain complex
20. Polo-like kinases: A team that plays throughout mitosis
21. Cell cycle regulation of the activity and subcellular localization of P1k1, a human protein kinase implicated in mitotic spindle function
22. Ran induces spindle assembly by reversing the inhibitory effect of importin α on TPX2 activity
23. Polo-like kinase is a cell cycle-regulated kinase activated during mitosis
24. Motor function in the mitotic spindle
25. Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts
26. Spindle assembly in Xenopus egg extracts: Respective roles of centrosomes and microtubule self-organization
27. In vitro assays for mitotic spindle assembly and function
28. A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro
29. The microtubule-destabilizing activity of metablastin (p19) is controlled by phosphorylation
30. Decreasing oncoprotein 18/ stathmin levels reduces microtubule catastrophes and increases microtubule polymer in vivo
31. Dissociation of the tubulin-sequestering and microtubule catastrophe-promoting activities of oncoprotein 18/stathmin
32. Centrosomes: Sic transit gloria centri
33. Stathmin: A tubulin-sequestering protein which forms a ternary T-2S complex with two tubulin molecules
34. Phosphatase 2A and Polo kinase, two antagonistic regulators of Cdc25 activation and MPF auto-amplification
35. PKA and MPF-activated polo-like kinase regulate anaphase- promoting complex activity
36. Purification and molecular cloning of P1x1, a Cdc25-regulatory kinase from Xenopus egg extracts
37. The Xenopus Chk1 protein kinase mediates a caffeine-sensitive pathway of checkpoint control in cell-free extracts
38. Antibody microinjection reveals as essential role for human polo-like kinase 1 (Ptk1) in the functional maturation of mitotic centrosomes
39. Control of microtubule dynamics by oncoprotein 18: Dissection of the regulatory role of multisite phosphorylation during mitosis
40. Microtubule dynamics: If you need a shrink try stathmin/Op18
41. Polo encodes a protein kinase homolog required for mitosis in Drosophila
42. The Drosophila POLO kinase localises to multiple compartments of the mitotic apparatus and is required for the phosphorylation of MPM2 reactive epitopes
43. Regulation of phosphoprotein p18 in leukemic cells: Cell cycle regulated phosphorylation by p34-cdc2 kinase
44. Oncoprotein 18 is a phosphorylation-responsive regulator of microtubule dynamics
45. Stathmin gene family: Phylogenetic conservation and developmental regulation in Xenopus
46. Chromosomal control of meiotic cell division
47. In vivo localisation of the mitotic POLO kinase shows a highly dynamic association with the mitotic apparatus during early embryogenesis in Drosophila
48. Cell cycle extracts
49. Importin β is a mitotic target of the small GTPase ran in spindle assembly
50. Polo-like kinases: Positive regulators of cell division from start to finish
51. The conserved Schizosaccharomyces pombe kinase plo1, required to form a bipolar spindle, the actin ring, and septum, can drive septum formation in G1 and G2 cells
52. Activated polo-like kinase P1x1 is required at multiple points during mitosis in Xenopus laevis
53. Mitotic effects of a constitutively active mutant of the Xenopus polo-like kinase P1x1
54. Transcriptional repression by nucleosomes but not H1 in reconstituted preblastoderm Drosophila chromatin
55. Mitotic spindle assembly by two different pathways in vitro
56. Normal development of mice lacking metablastin (P19), a phosphoprotein implicated in cell cycle regulation
57. The Polo-like kinase Cdc5p and the WD-repeat protein Cdc20p/fizzy are regulators and substrates of the anaphase promoting complex in Saccharomyces cerevisiae
58. Op18/stathmin caps a kinked protofilament-like tubulin tetramer
59. Polo, a mitotic mutant of Drosophila displaying abnormal spindle poles
60. DNA topoisomerase II-alpha is the major chromosome protein recognized by the mitotic phosphoprotein antibody MPM-2
61. Distinct roles of PP1 and PP2A-like phosphatases in control of microtubule dynamics during mitosis
62. Xk1p1, a chromosomal Xenopus kinesin-like protein essential for spindle organization and chromosome positioning
63. A model for the proposed roles of different microtubule-based motor proteins in establishing spindle bipolarity
64. Model for stathmin/ OP18 binding to tubulin
65. Mouse polo-like kinase 1 associates with the acentriolar spindle poles, meiotic chromosomes and spindle midzone during oocyte maturation
66. Role of importin-β in coupling ran to downstream targets in microtubule assembly
67. Silver staining of proteins in polyacrylamide gels
68. Chromosomes initiate spindle assembly upon experimental dissolution of the nuclear envelope in grasshopper spermatocytes