Structure of proline 3-hydroxylase: Evolution of the family of 2-oxoglutarate dependent oxygenases

European Journal of Biochemistry

Volumn 268, Issue 24, 2001, Pages 6625-6636

  Clifton, Ian J ^a   Hsueh, Li Ching ^a   Baldwin, Jack E ^a   Harlos, Karl ^b   Schofield, Christopher J ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

2 oxo glutarate; Hydroxyproline; Nonhaem iron.; Oxygenases; Proline 3 hydroxylase

Indexed keywords

2 OXOGLUTARIC ACID; AMINO ACID; ASCORBIC ACID; BACTERIAL ENZYME; IRON; LYSINE; OXYGENASE; PROLINE; PROLINE 3 HYDROXYLASE; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CRYSTAL STRUCTURE; ENZYME MECHANISM; ENZYME STRUCTURE; HYDROXYLATION; MOLECULAR EVOLUTION; OXIDATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN METABOLISM; PROTEIN MOTIF; STREPTOMYCES; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; EVOLUTION, MOLECULAR; KETOGLUTARIC ACIDS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROCOLLAGEN-PROLINE DIOXYGENASE; PROLINE; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY, AMINO ACID;

BACTERIA (MICROORGANISMS); STREPTOMYCES; STREPTOMYCES SP.;

EID: 0035663669     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2001.02617.x     Document Type: Article

References (58)

1. Geometric and electronic structure/function correlations in non-heme iron enzymes
2. Structural and mechanistic studies on 2-oxoglutarate-dependent oxygenases and related enzymes
3. Oxygen activating nonheme iron enzymes
4. Dioxygen activation by enzymes with mononuclear non-heme iron active sites
5. Reactions of nonheme iron (II) centers with dioxygen in biology and chemistry
6. The 2-His-1-carboxylate facial triad-an emerging structural motif in mononuclear non-heme iron(II) enzymes
7. A dilemma of dioxygenases (or where biochemistry and molecular biology fail to meet)
8. Dioxygenases: Molecular structure and role in plant metabolism
9. Collagen hydroxylases and the protein disulfide isomerase subunit of prolyl 4-hydroxy-lases
10. Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase - This subunit and protein disulfide isomerase are products of the same gene
11. Characterization of the iron- and 2-oxoglutarate-binding sites of human prolyl 4-hydroxyl-ase
12. Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation
13. Protein disulfide isomerase acts as a molecular chaperone during the assembly of procollagen
14. Progress towards drugs to treat fibrotic diseases
15. The structure of etamycin
16. Purification and initial characterization of proline 4-hydroxylase from Streptomyces griseoviridus P8648: A 2-oxoacid, ferrous-dependent dioxygenase involved in etamycin biosynthesis
17. Detection of novel proline 3-hydroxylase activities in Streptomyces and Bacillus spp by regio- and stereospecific hydroxylation of L-proline
18. Purification and cloning of a proline 3-hydroxylase, a novel enzyme which hydroxylates free L-proline to cis-3-hydroxy-L-proline
19. Enzymatic production of trans-4-hydroxy-L-proline by regio- and stereospecific hydroxylation of L-proline
20. Studies on the active site of deacetoxycephalosporin C synthase
21. Structure of a cephalosporin synthase
22. Structural origins of the selectivity of the trifunctional oxygenase clavaminic acid synthase
23. Structure of isopenicillin N synthase is the first from a new structural family of enzymes
24. Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation
25. Processing of X-ray diffraction data collected in oscillation mode
26. The CCP4 suite: Programs for protein crystallography
27. Detecting and overcoming crystal twinning
28. Trimethyllead acetate: A first choice heavy atom derivative for protein crystallography
29. XtalView Xfit - A versatile program for manipulating atomic coordinates and electron density
30. Maximum-likelihood heavy atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods
31. Phase combination and cross validation in iterated density-modification calculations
32. Refinement of macromolecular structures by the maximum-likelihood method
33. Crystallography & NMR system: A new software system for macromolecular structure determination
34. Protein structure comparison by alignment of distance matrices
35. Unanticipated inhibition of the metallo-beta-lactamase from Bacteroides fragilis by 4-morpholineethanesulfonic acid (MES): A crystallographic study at 1.85-angstrom resolution
36. Glutamine-330 is not essential for activity in isopenicillin N synthase from Aspergillus nidulans
37. The glutamine ligand in the ferrous iron active site of isopenicillin N synthase of Streptomyces jumonjinensis is not essential for catalysis
38. A kinetic study of thymine 7-hydroxylase from Neurospora grassa
39. Substrate binding to the alpha-ketoglutarate-dependent non-heme iron enzyme clavaminate synthase 2: Coupling mechanism of oxidative decarboxylation and hydroxylation
40. Circular dichroism and magnetic circular dichroism spectroscopic studies of the non-heme ferrous active site in clavaminate synthase and its interaction with alpha-ketoglutarate cosubstrate
41. A stereochemical concept for the catalytic mechanism of prolylhydroxylase. Applicability to classification and design of inhibitors
42. 18O-labeled water into oxygenated products produced by the enzyme deacetoxy deacetylcephalosporin C synthase
43. Two isozymes of clavaminate synthase central to clavulanic acid formation - Cloning and sequencing of both genes from Streptomyces clavuligerus
44. Expression in Escherichia coli of a clavaminic acid synthase isozyme - A trifunctional oxygenase involved in clavulanic acid biosynthesis
45. Crystal structure of Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase: An enzyme involved in the tyrosine degradation pathway
46. Stereochemical course of the conversion of β-ketoisocaproate to b-hydroxyisovalerate by soluble, recombinant mammalian 4-hydroxyphenylpyruvate dioxy-genase
47. Substrate-specificity of proline 4-hydroxylase - Chemical and enzymatic-synthesis of 2S,3R,4S-epoxyproline
48. Substrate selectivities of proline hydroxylases
49. Molscript - A program to produce both detailed and schematic plots of protein structures
50. Protein structural topology: Automated analysis and diagrammatic representation
51. An extensively modified version of MolScript that includes greatly enhanced coloring capabilities
52. Raster3D: Photorealistic molecular graphics
53. Mechanism-based inactivation of the human prolyl-4-hydroxylase by 5-oxaproline-containing peptides: Evidence for a prolyl radical intermediate
54. Studies on the mechanism of deacetoxy deacetylcephalo-sporin-C synthase using cyclopropyl substituted cephalosporin probes
55. A method for the systematic comparison of the three-dimensional structures of proteins and some results
56. Protein sequence alignments: A strategy for the hierarchical analysis of residue conservation