Transient expression of wild-type or biologically inactive telomerase allows the formation of artificial telomeres in mortal human cells

Experimental Cell Research

Volumn 265, Issue 2, 2001, Pages 304-311

  Guiducci, Cristiana ^a   Anglana, Mauro ^a   Wang, Arthur ^a   Bacchetti, Silvia ^a  

^a MCMASTER UNIVERSITY   (Canada)

Author keywords

hTERT; SV40 transformed cells; Telomerase; Telomere seeding

Indexed keywords

RNA DIRECTED DNA POLYMERASE; TELOMERASE;

AMINO ACID SEQUENCE; ARTICLE; CELL IMMORTALIZATION; CHROMOSOME STRUCTURE; CONTROLLED STUDY; DNA DETERMINATION; GENE EXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; TELOMERE;

EID: 0035325159     PISSN: 00144827     EISSN: None     Source Type: Journal    
DOI: 10.1006/excr.2001.5189     Document Type: Article

References (52)

1. The DNA structures at the ends of eukaryotic chromosomes
2. Mammalian telomeres end in a large duplex loop
3. Control of human telomere length by TRF1 and TRF2
4. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts
5. The telomerase reverse transcriptase: Components and regulation
6. Telomerase catalytic subunit homologs from fission yeast and human
7. hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization
8. Isolation of a candidate human telomerase catalytic subunit gene, which reveals complex splicing patterns in different cell types
9. Human telomerase contains evolutionarily conserved catalytic and structural subunits
10. Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT
11. Telomerase activity is restored in human cells by ectopic expression of hTERT (hEST2), the catalytic subunit of telomerase
12. Reconstitution of human telomerase activity in vitro
13. Telomeres shorten during ageing of human fibroblasts
14. Principle of marginotomy in template synthesis of polynucleotides
15. Origins of concatemeric T7 DNA
16. A theory of marginotomy. The incomplete copying of template margin in enzymic synthesis of polynucleotides and biological significance of the phenomenon
17. Extension of life-span by introduction of telomerase into normal human cells
18. Reconstitution of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span
19. Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity
20. p53- and ATM-dependent apoptosis induced by telomeres lacking TRF2
21. Inhibition of telomerase limits the growth of human cancer cells
22. Telomere shortening and apoptosis in telomerase-inhibited human tumor cells
23. Specific association of human telomerase activity with immortal cells and cancer
24. Telomerase activity in human ovarian carcinoma
25. Non-programmed and engineered chromosome breakage
26. Mammalian telomeres and chromosome fragmentation
27. Stringent sequence requirements for the formation of human telomeres
28. A human telomeric protein
29. Human telomeres contain two distinct Myb-related proteins, TRF1 and TRF2
30. Telomeric localization of TRF2, a novel human telobox protein
31. Telomere directed fragmentation of mammalian chromosomes
32. Functional reintroduction of human telomeres into mammalian cells
33. Telomere-associated chromosome fragmentation: Applications in genome manipulation and analysis
34. Targeted breakage of a human chromosome mediated by cloned human telomeric DNA
35. Acquisition of telomere repeat sequences by transfected DNA integrated at the site of a chromosome break
36. Cooperative effect of antisense-Rb and antisense p53 on the extension of life span in human diploid fibroblasts
37. A role for both pRB and p53 in the regulation of human cellular senescence
38. Telomeres-Unsticky ends
39. Ku binds telomeric DNA in vitro
40. Ku is associated with the telomere in mammals
41. DNA double-strand break repair proteins are required to cap the ends of mammalian chromosomes
42. MRT-2 checkpoint protein is required for germline immortality and telomere replication in C. elegans
43. TRF1 is a critical trans-acting factor for de novo telomere formation in human cells
44. Telomere maintenance by recombination in human cells
45. Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization
46. Telomerase activity does not always imply telomere maintenance
47. Telomerase extends the lifespan of virus-transformed human cells without net telomere lengthening
48. Techniques for human adenovirus vector construction and characterization
49. An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3
50. Expression of SV40 large T antigen, but not small t antigen, is required for the induction of chromosomal aberrations in transformed human cells
51. Construction of a recombinant adenovirus for efficient delivery of the I-SceI yeast endonuclease to human cells and its application in the in vivo cleavage of chromosomes to expose new potential telomeres
52. Reprogramming of telomerase by expression of mutant telomerase RNA template in human cells leads to altered telomeres that correlate with reduced cell viability