Reprogramming of telomerase by expression of mutant telomerase RNA template in human cells leads to altered telomeres that correlate with reduced cell viability

Molecular and Cellular Biology

Volumn 17, Issue 11, 1997, Pages 6394-6401

  Marušić, Lidija ^a   Anton, Martina ^a   Tidy, Antonella ^a   Wang, Ping ^a   Villeponteau, Bryant ^b   Bacchetti, Silvia ^a  

^a MCMASTER UNIVERSITY   (Canada)

^b GERON CORPORATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; NEOMYCIN; RNA; TELOMERASE;

ARTICLE; CELL VIABILITY; DNA TEMPLATE; ENZYME ACTIVITY; GENE; GROWTH RATE; HUMAN; HUMAN CELL; PLASMID; PRIORITY JOURNAL; SENESCENCE; TELOMERE;

EID: 1842339879     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.17.11.6394     Document Type: Article

References (63)

1. Adams, R. R. Personal communication.
2. Allshire, R. C., M. Dempster, and N. Hastie. 1989. Human telomeres contain at least three types of G-rich repeats distributed non-randomly. Nature 332:656-659.
3. Allsopp, R. C., H. Vaziri, C. Patterson, S. Goldstein, E. V. Younglai, A. B. Futcher, C. W. Greider, and C. B. Harley. 1992. Telomere length predicts replicative capacity of human fibroblasts. Proc. Natl. Acad. Sci. USA 89:10114-10118.
4. Avilion, A. A. 1995. Ph.D. thesis. State University of New York, Stony Brook.
5. Bacchetti, S. 1996. Telomere dynamics and telomerase activity in cell senescence and cancer. Semin. Cell Biol. 7:423-432.
6. Bacchetti, S., and C. M. Counter. 1995. Telomeres and telomerase in human cancer. Int. J. Oncol. 7:31-39.
7. Biessmann, H., and J. M. Mason. 1992. Genetics and molecular biology of telomeres. Adv. Genet. 30:185-249.
8. Bryan, T. M., A. Englezou, J. Gupta, S. Bacchetti, and R. R. Reddel. 1995. Telomere elongation in immortal human cells without detectable telomerase activity. EMBO J. 14:4240-4248.
9. Bryan, T. M., L. Marušić, S. Bacchetti, M. Namba, and R. R. Reddel. 1997. The non-telomerase telomere lengthening mechanism in immortal human cells does not involve the telomerase RNA subunit. Hum. Mol. Genet. 6:921-926.
10. Bryan, T. M., and R. R. Reddel. 1997. Telomere dynamics and telomerase activity in in vitro immortalized cells. Eur. J. Cancer 33:767-773.
11. Busch, H., R. Reddy, L. Rothblum, and Y. C. Choi. 1982. SnRNAs, SnRNPs, and RNA processing. Annu. Rev. Biochem. 51:617-654.
12. Chong, L., B. van Steensel, D. Broccoli, H. Erdjument-Bromage, J. Hanish, P. Tempst, and T. de Lange. 1995. A human telomeric protein. Science 270:1663-1667.
13. Cooper, J. P., E. R. Nimmo, R. C. Allshire, and T. R. Cech. 1997. Regulation of telomere length and function by a Myb-domain protein in fission yeast. Nature 385:744-747.
14. Counter, C. M., A. A. Avilion, C. E. LeFeuvre, N. G. Stewart, C. W. Greider, C. B. Harley, and S. Bacchetti. 1992. Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. EMBO J. 11:1921-1929.
15. Counter, C. M., F. M. Botelho, P. Wang, C. B. Harley, and S. Bacchetti. 1994. Stabilization of short telomeres and telomerase activity accompany immortalization of Epstein-Barr virus-transformed human B lymphocytes. J. Virol. 68:3410-3414.
16. Conter, C. M, H. W. Hirte, S. Bacchetti, and C. B. Harley. 1994. Telomerase activity in human ovarian carcinoma. Proc. Natl. Acad. Sci. USA 91:2900-2904.
17. de Lange, T. 1955. Telomere dynamics and genome instability in human cancer, p. 265-293. In E. H. Blackburn and C. W. Greider (ed.), Telomeres. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
18. de Lange, T. Personal communication.
19. Dimri, G. P., X. Lee, G. Basile, M. Acosta, G. Scott, C. Roskelley, E. E. Medrano, M. Linskens, I. Rubelj, O. Pereira-Smith, M. Peacocke, and J. Campisi. 1995. A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc. Natl. Acad. Sci. USA 92:9363-9367.
20. Fang, G., and T. R. Cech. 1995. Telomere proteins, p. 69-105. In E. H. Blackburn and C. W. Greider (ed.), Telomeres. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
21. Feng, J., W. D. Funk, S. S. Wang, S. L. Weinrich, A. A. Avilion, C. P. Chiu, R. R. Adams, E. Chang, R. C. Allsopp, J. Yu, S. Le, M. D. West, C. B. Harley, W. H. Andrews, C. W. Greider, and B. Villeponteau. 1995. The RNA component of human telomerase. Science 269:1236-1241.
22. Graham, F. L., and A. J. van der Eb. 1973. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456-467.
23. Graham, F. L., J. R. Smiley, W. C. Russell, and R. Nairn. 1977. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36:59-72.
24. Greider, C. W. 1996. Telomere length regulation. Annu. Rev. Biochem. 65:337-365.
25. Greider, C. W., and E. H. Blackburn. 1985. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell 43:405-413.
26. Greider, C. W., and E. H. Blackburn. 1989. A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis. Nature 337:331-337.
27. Hanish, J. P., J. Yanowitz, and T. de Lange. 1994. Stringent sequence requirements for telomere formation in human cells. Proc. Natl. Acad. Sci. USA 91:8861-8865.
28. Harley, C. B., A. B. Futcher, and C. W. Greider. 1990. Telomeres shorten during ageing of human fibroblasts. Nature 345:458-460.
29. Hayflick, L. 1965. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37:614-636.
30. Holt, S. E., J. C. Norton, W. E. Wright, and J. W. Shay. 1996. Comparison of the telomeric repeat amplification protocol (TRAP) to the new TRAP-eze telomerase detection kit. Methods Cell Sci. 18:237-248.
31. Holt, S. E., W. E. Wright, and J. W. Shay. 1997. Multiple pathways for the regulation of telomerase activity. Eur. J. Cancer 33:761-766.
32. Kim, N.-W., and F. Wu. 1997. Advances in quantification and characterization of telomerase activity by the telomeric repeat amplificaiton protocol (TRAP). Nucleic Acids Res. 25:2595-2597.
33. Kim, N.-W., M. A. Piatyszek, K. R. Prowse, C. B. Harley, M. D. West, P. L. C. Ho, G. M. Coviello, W. E. Wright, S. L. Weinrich, and J. W. Shay. 1994. Specific association of human telomerase activity with immortal cells and cancer. Science 266:2011-2015.
34. Kirk, K. E., B. P. Harmon, I. K. Reichardt, J. W. Sedat, and E. H. Blackburn. 1997. Block in anaphase chromosome separation caused by a telomerase template mutation. Science 275:1478-1481.
35. Klingelhutz, A. J., S. A. Barber, P. Smith, K. Dyer, and J. K. McDougall. 1994. Restoration of telomeres in human papillomavirus-immortalized human anogenital epithelial cells. Mol. Cell. Biol. 14:961-969.
36. Kyrion, G., K. A. Boakye, and A. J. Lustig. 1992. C-terminal truncation of RAP1 results in the deregulation of telomere size, stability, and function in Saccharomyces cerevisiae. Mol. Cell. Biol. 12:5159-5173.
37. Lundblad, V., and J. W. Szostak. 1989. A mutant with a defect in telomere elongation leads to senescence in yeast. Cell 57:633-643.
38. Lundblad, V., and E. H. Blackburn. 1993. An alternative pathway for yeast telomere maintenance rescues est1 senescence in yeast. Cell 73:347-360.
39. Mangin, M., M. Ares, Jr., and A. M. Weiner. 1986. Human U2 small nuclear RNA genes contain an upstream enhancer. EMBO J. 5:987-995.
40. Marušić, L. Unpublished data.
41. McEachern, M. J., and E. H. Blackburn. 1995. Runaway telomere elongation caused by telomerase RNA gene mutations. Nature 376:403-409.
42. McEachern, M. J., and E. H. Blackburn. 1996. Cap-prevented recombination between terminal telomeric repeat arrays (telomere CPR) maintains telomeres in Kluyveromyces lactis lacking telomerase. Genes Dev. 10:1822-1834.
43. Morin, G. B. 1989. The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats. Cell 59:521-529.
44. n, present at the telomeres of human chromosomes. Proc. Natl. Acad. Sci. USA 85:6622-6626.
45. Murnane, J. P., L. Sabatier, B. A. Marder, and W. F. Morgan. 1994. Telomere dynamics in an immortal human cell line. EMBO J. 13:4953-4962.
46. Ohmura, H., H. Tahara, M. Suzuki, T. Ide, M. Shimizu, M. A. Yoshida, E. Tahara, J. W. Shay, J. C. Barrett, and M. Oshimura. 1995. Restoration of the cellular senescence program and repression of telomerase by human chromosome 3. Jpn. J. Cancer Res. 86:899-904.
47. Olovnikov, A. M. 1971. Principle of marginotomy in template synthesis of polynucleotides. Dokl. Biochem. 201:394-397.
48. Olovnikov, A. M. 1973. A theory of marginotomy: the incomplete copying of template margin in enzymatic synthesis of polynucleotides and biological significance of the phenomenon. J. Theor. Biol. 41:181-190.
49. Prescott, J., and E. H. Blackburn. 1997. Telomerase RNA mutations in Saccharomyces cerevisiae alter telomerase action and reveal nonprocessivity in vivo and in vitro. Genes Dev. 11:528-540.
50. Rasheed, S., W. A. Nelson-Reed, E. M. Toth, P. Arnstein, and M. B. Gardner. 1974. Characterization of a newly derived human sarcoma cell line (HT-1080). Cancer 33:1027-1033.
51. Shay, J. W., G. Tomlinson, M. A. Piatyszek, and L. S. Gollahon. 1995. Spontaneous in vitro immortalization of breast epithelial cells from a patient with Li-Fraumeni syndrome. Mol. Cell. Biol. 15:425-432.
52. Singer, M. S., and D. E. Gottschling. 1994. TLC1, the template RNA component of Saccharomyces cerevisiae telomerase. Science 266:387-388.
53. Strahl, C., and E. H. Blackburn. 1996. Effects of reverse transcriptase inhibitors on telomere length and telomerase activity in two immortalized human cell lines. Mol. Cell. Biol. 16:53-65.
54. van Steensel, B., and T. de Lange. 1997. Control of telomere length by the human telomeric protein TRF1. Nature 385:740-743.
55. Virta-Pearlman, V., D. K. Morris, and V. Lundblad. 1996. Est1 has the properties of a single-stranded telomere end-binding protein. Genes Dev. 10:3094-3104.
56. Wang, S.-S., and V. A. Zakian. 1990. Telomere-telomere recombination provides an express pathway for telomere acquisition. Nature 345:446-458.
57. Watson, J. D. 1972. Origins of concatemeric T7 DNA. Nature New Biol. 239:197-201.
58. Wright, W. E., M. A. Piatyszek, W. E. Rainey, W. Byrd, and J. W. Shay. 1996. Telomerase activity in human germline and embryonic tissues and cells. Dev. Genet. 18:173-179.
59. Wright, W. E., D. Brasiskyte, M. A. Piatyszek, and J. W. Shay. 1996. Experimental elongation of telomeres extends the lifespan of immortal x normal cell hybrids. EMBO J. 15:1734-1741.
60. Yu, G., J. D. Bradley, L. D. Attardi, and E. H. Blackburn. 1990. In vivo alteration of telomere sequences and senescence caused by mutated Tetrahymena telomerase RNAs. Nature 344:126-131.
61. Zakian, V. A. 1995. Telomeres: beginning to understand the end. Science 270:1601-1607.
62. Zhong, Z., L. Shiue, S. Kaplan, and T. de Lange. 1992. A mammalian factor that binds telomeric TTAGGG repeats in vitro. Mol. Cell. Biol. 12:4834-4843.
63. Zhu, X., R. Kumar, M. Mandai, N. Sharma, H. W. Sharma, U. Dhingra, J. A. Sokoloski, R. Hsiao, and R. Narayanan. 1996. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93:6091-6095.