Selection of stabilized 3-isopropylmalate dehydrogenase of Saccharomyces cerevisiae using the host-vector system of an extreme thermophile, Thermus thermophilus

Extremophiles

Volumn 5, Issue 1, 2001, Pages 17-22

  Tamakoshi, Masatada ^a   Nakano, Yumiko ^a   Kakizawa, Shigeyuki ^a   Yamagishi, Akihiko ^a   Oshima, Tairo ^a  

^a TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

Author keywords

3 Isopropylmalate dehydrogenase; Evolutionary engineering; Saccharomyces cerevisiae; Thermostability; Thermus thermophilus

Indexed keywords

3 ISOPROPYLMALATE DEHYDRATASE; 3-ISOPROPYLMALATE DEHYDRATASE; GLYCEROL; HYDROLYASE; LEUCINE;

ARTICLE; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; FUNGAL GENE; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; HEAT; KINETICS; METABOLISM; MOLECULAR CLONING; MUTATION; PROTEIN DENATURATION; PROTEIN SECONDARY STRUCTURE; SACCHAROMYCES CEREVISIAE; THERMUS THERMOPHILUS;

CLONING, MOLECULAR; ENZYME STABILITY; GENES, FUNGAL; GENETIC VECTORS; GLYCEROL; HEAT; HYDRO-LYASES; KINETICS; LEUCINE; MUTATION; PROTEIN DENATURATION; PROTEIN STRUCTURE, SECONDARY; SACCHAROMYCES CEREVISIAE; THERMUS THERMOPHILUS; TRANSFORMATION, GENETIC;

EID: 0035259956     PISSN: 14310651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s007920000168     Document Type: Article

References (28)

1. Akanuma S, Yamagishi A, Tanaka N, Oshima T (1996) Spontaneous tandem sequence duplications reverse the thermal stability of carboxyl-terminal modified 3-isopropylmalate dehydrogenase. J Bacteriol 178:6300-6304
2. Akanuma S, Yamagishi A, Tanaka N, Oshima T (1998) Serial increase in the thermal stability of 3-isopropylmalate dehydrogenase from Bacillus subtilis by experimental evolution. Protein Sci 7:698-705
3. Akanuma S, Yamagishi A, Tanaka N, Oshima T (1999) Further improvement of the thermal stability of a partially stabilized Bacillus subtilis 3-isopropylmalate dehydrogenase variant by random and site-directed mutagenesis. Eur J Biochem 260:499-504
4. Broach JR, Strathern JN, Hicks JB (1979) Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene (Amst) 8:121-123
5. Giver L, Gershenson A, Freskgard PO, Arnold FH (1998) Directed evolution of a thermostable esterase. Proc Natl Acad Sci USA 95:12809-12813
6. Hayashi-Iwasaki Y, Numata K, Yamagishi A, Yutani K, Sakurai M, Tanaka N, Oshima T (1996) A stable intermediate in the thermal unfolding process of a chimeric 3-isopropylmalate dehydrogenase between a thermophilic and a mesophilic enzyme. Protein Sci 5:511-516
7. Hoseki J, Yano T, Koyama Y, Kuramitsu S, Kagamiyama H (1999) Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus. J Biochem (Tokyo) 126:951-956
8. Hsu YP, Kohlhaw GB (1980) Leucine biosynthesis in Saccharomyces cerevisiae. Purification and characterization of ß-isopropylmalate dehydrogenase. J Biol Chem 255:7255-7260
9. Imada K, Sato M, Tanaka N, Katsube Y, Matsuura Y, Oshima T (1991) Three-dimensional structure of a highly thermostable enzyme, 3-isopropylmalate dehydrogenase of Thermus thermophilus at 2.2 Å resolution. J Mol Biol 222:725-738
10. Imada K, Inagaki K, Matsunami H, Kawaguchi H, Tanaka H, Tanaka N, Namba K (1998) Structure of 3-isopropylmalate dehydrogenase in complex with 3-isopropylmalate at 2.0 Å resolution: the role of Glu88 in the unique substrate-recognition mechanism. Structure (Lond) 6:971-982
11. Kirino H, Aoki M, Aoshima M, Hayashi Y, Ohba M, Yamagishi A, Wakagi T, Oshima T (1994) Hydrophobic interaction at the subunit interface contributes to the thermostability of 3-isopropylmalate dehydrogenase from an extreme thermophile, Thermus thermophilus. Eur J Biochem 220:275-281
12. Kohlhaw GB (1988) ß-Isopropylmalate dehydrogenase from yeast. Methods Enzymol 166:429-435
13. Kotsuka T, Akanuma S, Tomuro M, Yamagishi A, Oshima T (1996) Further stabilization of 3-isopropylmalate dehydrogenase of an extreme thermophile, Thermus thermophilus, by a suppressor mutation method. J Bacteriol 178:723-727
14. Koyama Y, Hoshino T, Tomizuka N, Furukawa K (1986) Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp. J Bacteriol 166:338-340
15. Miyazaki K, Wintrode PL, Grayling RA, Rubingh DN, Arnold FH (2000) Directed evolution study of temperature adaptation in a psychrophilic enzyme. J Mol Biol 297:1015-1026
16. Picard V, Ersdal BE, Lu A, Bock SC (1994) A rapid and efficient one-tube PCR-based mutagenesis technique using Pfu DNA polymerase. Nucleic Acids Res 22:2587-2591
17. Priev A, Almagor A, Yedgar S, Gavish B (1996) Glycerol decreases the volume and compressibility of protein interior. Biochemistry 35:2061-2066
18. Qu C, Akanuma S, Moriyama H, Tanaka N, Oshima T (1997) A mutation at the interface between domains causes rearrangement of domains in 3-isopropylmalate dehydrogenase. Protein Eng 10:45-52
19. Sakurai M, Moriyama H, Onodera K, Kadono S, Numata K, Hayashi Y, Kawaguchi J, Yamagishi A, Oshima T, Tanaka N (1995) The crystal structure of thermostable mutants of chimeric 3-isopropylmalate dehydrogenase, 2T2M6T. Protein Eng 8:763-767
20. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
21. Spiller B, Gershenson A, Arnold FH, Stevens RC (1999) A structural view of evolutionary divergence. Proc Natl Acad Sci USA 96:12305-12310
22. Stemmer WP (1994) Rapid evolution of a protein in vitro by DNA shuffling. Nature (Lond) 370:389-391
23. Tamakoshi M, Yamagishi A, Oshima T (1995) Screening of stable proteins in an extreme thermophile, Thermus thermophilus. Mol Microbiol 16:1031-1036
24. Tamakoshi M, Uchida M, Tanabe K, Fukuyama S, Yamagishi A, Oshima T (1997) A new Thermus-Escherichia coli shuttle integration vector system. J Bacteriol 179:4811-4814
25. Tamakoshi M, Yamagishi A, Oshima T (1998) The organization of the leuC, leuD and leuB genes of the extreme thermophile Thermus thermophilus. Gene (Amst) 222:125-132
26. Tamakoshi M, Yaoi T, Oshima T, Yamagishi A (1999) An efficient gene replacement and deletion system for an extreme thermophile, Thermus thermophilus. FEMS Microbiol Lett 173:431-437
27. Wallon G, Kryger G, Lovett ST, Oshima T, Ringe D, Petsko GA (1997) Crystal structures of Escherichia coli and Salmonella typhimurium 3-isopropylmalate dehydrogenase and comparison with their thermophilic counterpart from Thermus thermophilus. J Mol Biol 266:1016-1031
28. Zhao H, Arnold FH (1999) Directed evolution converts subtilisin E into a functional equivalent of thermitase. Protein Eng 12:47-53