A stable intermediate in the thermal unfolding process of a chimeric 3-isopropylmalate dehydrogenase between a thermophilic and a mesophilic enzymes

Protein Science

Volumn 5, Issue 3, 1996, Pages 511-516

  Hayashi Iwasaki, Yoko ^a,c   Numata, Koichi ^b   Yamagishi, Akihiko ^c   Yutani, Katsuhide ^d   Sakurai, Masahiro ^a   Tanaka, Nobuo ^a   Oshima, Tairo ^c  

^a TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^b TOYOTA MOTOR CORPORATION   (Japan)

^c TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

^d OSAKA UNIVERSITY   (Japan)

Author keywords

Chimeric protein; Circular dichroism; Domain; Scanning microcalorimetry; Thermal stability; Thermus thermophilus

Indexed keywords

3 ISOPROPYLMALATE DEHYDROGENASE; CHIMERIC PROTEIN;

ARTICLE; BACILLUS SUBTILIS; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME CONFORMATION; ENZYME STABILITY; MICROCALORIMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING; THERMOSTABILITY; THERMUS THERMOPHILUS;

3-ISOPROPYLMALATE DEHYDROGENASE; ALCOHOL OXIDOREDUCTASES; BACILLUS SUBTILIS; CALORIMETRY, DIFFERENTIAL SCANNING; CIRCULAR DICHROISM; COMPUTER GRAPHICS; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; TEMPERATURE; THERMUS THERMOPHILUS;

EID: 0029881741     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560050313     Document Type: Article

References (15)

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