-
1
-
-
0023392267
-
A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains
-
Alani, E., L. Cao, and N. Kleckner. 1987. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116:541-545.
-
(1987)
Genetics
, vol.116
, pp. 541-545
-
-
Alani, E.1
Cao, L.2
Kleckner, N.3
-
2
-
-
0021712245
-
Cloning gene ura5 for the orotidylic acid pyrophosphorylase of the filamentous fungus Podospora anserina: Transformation of protoplasts
-
Begueret, J., V. Razanamparany, M. Perrot, and C. Barreau. 1984. Cloning gene ura5 for the orotidylic acid pyrophosphorylase of the filamentous fungus Podospora anserina: transformation of protoplasts. Gene 32:487-492.
-
(1984)
Gene
, vol.32
, pp. 487-492
-
-
Begueret, J.1
Razanamparany, V.2
Perrot, M.3
Barreau, C.4
-
3
-
-
0025305971
-
Construction of an integration vector for use in the archaebacterium Methanococcus voltae and expression of a eubacterial resistance gene
-
Gernhardt, P., O. Possot, M. Foglino, L. Sibold, and A. Klein. 1990. Construction of an integration vector for use in the archaebacterium Methanococcus voltae and expression of a eubacterial resistance gene. Mol. Gen. Genet. 221:273-279.
-
(1990)
Mol. Gen. Genet.
, vol.221
, pp. 273-279
-
-
Gernhardt, P.1
Possot, O.2
Foglino, M.3
Sibold, L.4
Klein, A.5
-
4
-
-
0025321953
-
The development of a heterologous transformation system for the cellulolytic fungus Trichoderma reesei based on a pyrG-negative mutant strain
-
Gruber, F., J. Visser, C. P. Kubicek, and L. H. de Graaff. 1990. The development of a heterologous transformation system for the cellulolytic fungus Trichoderma reesei based on a pyrG-negative mutant strain. Curr. Genet. 18:71-76.
-
(1990)
Curr. Genet.
, vol.18
, pp. 71-76
-
-
Gruber, F.1
Visser, J.2
Kubicek, C.P.3
De Graaff, L.H.4
-
5
-
-
0027435913
-
Plasmid marker rescue transformation in Thermus thermophilus
-
Hoshino, T., H. Maseda, and T. Nakahara. 1993. Plasmid marker rescue transformation in Thermus thermophilus. J. Ferment. Bioeng. 76:276-279.
-
(1993)
J. Ferment. Bioeng.
, vol.76
, pp. 276-279
-
-
Hoshino, T.1
Maseda, H.2
Nakahara, T.3
-
6
-
-
0030028478
-
Further stabilization of 3-isopropylmalate dehydrogenase of an extreme thermophile, Thermus thermophilus, by a suppressor mutation method
-
Kotsuka, T., S. Akanuma, M. Tomuro, A. Yamagishi, and T. Oshima. 1996. Further stabilization of 3-isopropylmalate dehydrogenase of an extreme thermophile, Thermus thermophilus, by a suppressor mutation method. J. Bacteriol. 178:723-727.
-
(1996)
J. Bacteriol.
, vol.178
, pp. 723-727
-
-
Kotsuka, T.1
Akanuma, S.2
Tomuro, M.3
Yamagishi, A.4
Oshima, T.5
-
7
-
-
0025114294
-
A plasmid vector for an extreme thermophile, Thermus thermophilus
-
Koyama, Y., Y. Arikawa, and K. Furukawa. 1990. A plasmid vector for an extreme thermophile, Thermus thermophilus. FEMS Microbiol. Lett. 60:97-101.
-
(1990)
FEMS Microbiol. Lett.
, vol.60
, pp. 97-101
-
-
Koyama, Y.1
Arikawa, Y.2
Furukawa, K.3
-
8
-
-
0022448655
-
Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp
-
Koyama, Y., T. Hoshino, N. Tomizuka, and K. Furukawa. 1986. Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp. J. Bacteriol. 166:338-340.
-
(1986)
J. Bacteriol.
, vol.166
, pp. 338-340
-
-
Koyama, Y.1
Hoshino, T.2
Tomizuka, N.3
Furukawa, K.4
-
9
-
-
0025336553
-
Cloning of α- and β-galactosidase genes from an extreme thermophile, Thermus strain T2, and their expression in Thermus thermophilus HB27
-
Koyama, Y., S. Okamoto, and K. Furukawa. 1990. Cloning of α-and β-galactosidase genes from an extreme thermophile, Thermus strain T2, and their expression in Thermus thermophilus HB27. Appl. Environ. Microbiol. 56: 2251-2254.
-
(1990)
Appl. Environ. Microbiol.
, vol.56
, pp. 2251-2254
-
-
Koyama, Y.1
Okamoto, S.2
Furukawa, K.3
-
10
-
-
2642699794
-
Rapid and efficient site-specific mutagenesis without phenotypic selection
-
Kunkel, T. A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82:488-492.
-
(1985)
Proc. Natl. Acad. Sci. USA
, vol.82
, pp. 488-492
-
-
Kunkel, T.A.1
-
11
-
-
0026752841
-
Insertional mutagenesis in the extreme thermophilic eubacteria Thermus thermophilus HB8
-
Lasa, I., J. R. Caston, H. L. Fernandez, M. de Pedro, and J. Berenguer. 1992. Insertional mutagenesis in the extreme thermophilic eubacteria Thermus thermophilus HB8. Mol. Microbiol. 6:1555-1564.
-
(1992)
Mol. Microbiol.
, vol.6
, pp. 1555-1564
-
-
Lasa, I.1
Caston, J.R.2
Fernandez, H.L.3
De Pedro, M.4
Berenguer, J.5
-
12
-
-
0026722505
-
Development of Thermus-Escherichia shuttle vectors and their use for expression of the Clostridium thermocellum celA gene in Thermus thermophilus
-
Lasa, I., M. de Grado, M. A. de Pedro, and J. Berenguer. 1992. Development of Thermus-Escherichia shuttle vectors and their use for expression of the Clostridium thermocellum celA gene in Thermus thermophilus. J. Bacteriol. 174:6424-6431.
-
(1992)
J. Bacteriol.
, vol.174
, pp. 6424-6431
-
-
Lasa, I.1
De Grado, M.2
De Pedro, M.A.3
Berenguer, J.4
-
13
-
-
0342367772
-
Isolation of a thermostable enzyme variant by cloning and selection in a thermophile
-
Liao, H., T. McKenzie, and R. Hageman. 1986. Isolation of a thermostable enzyme variant by cloning and selection in a thermophile. Proc. Natl. Acad. Sci. USA 83:576-580.
-
(1986)
Proc. Natl. Acad. Sci. USA
, vol.83
, pp. 576-580
-
-
Liao, H.1
McKenzie, T.2
Hageman, R.3
-
14
-
-
0026500944
-
Development of plasmid cloning vectors for Thermus thermophilus HB8: Expression of a heterologous, plasmid-borne kanamycin nucleotidyltransferase gene
-
Mather, M. W., and J. A. Fee. 1992. Development of plasmid cloning vectors for Thermus thermophilus HB8: expression of a heterologous, plasmid-borne kanamycin nucleotidyltransferase gene. Appl. Environ. Microbiol. 58:421-125.
-
(1992)
Appl. Environ. Microbiol.
, vol.58
, pp. 421-1125
-
-
Mather, M.W.1
Fee, J.A.2
-
15
-
-
0022403302
-
Screening for thermostable mutant of kanamycin nucleotidyltransferase by the use of a transformation system for a thermophile, Bacillus stearothermophilus
-
Matsumura, M., and S. Aiba. 1985. Screening for thermostable mutant of kanamycin nucleotidyltransferase by the use of a transformation system for a thermophile, Bacillus stearothermophilus. J. Biol. Chem. 260:15298-15303.
-
(1985)
J. Biol. Chem.
, vol.260
, pp. 15298-15303
-
-
Matsumura, M.1
Aiba, S.2
-
16
-
-
0022016296
-
Cloning and characterization of the pyrE gene and of PyrE::Mud1 (Ap lac) fusions from Salmonella typhimurium
-
Neuhard, J., E. Stauning, and R. A. Kelln. 1985. Cloning and characterization of the pyrE gene and of PyrE::Mud1 (Ap lac) fusions from Salmonella typhimurium. Eur. J. Biochem. 146:597-603.
-
(1985)
Eur. J. Biochem.
, vol.146
, pp. 597-603
-
-
Neuhard, J.1
Stauning, E.2
Kelln, R.A.3
-
17
-
-
0015955554
-
Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from a Japanese thermal spa
-
Oshima, T., and K. Imahori. 1974. Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from a Japanese thermal spa. Int. J. Syst. Bacteriol. 24:102-112.
-
(1974)
Int. J. Syst. Bacteriol.
, vol.24
, pp. 102-112
-
-
Oshima, T.1
Imahori, K.2
-
18
-
-
0026697675
-
Directed mutagenesis in an asporogenous methylotrophic yeast: Cloning, sequencing, and one-step gene disruption of the 3-isopropylmalate dehydrogenase gene (LEU2) of Candida boidinii to derive doubly auxotrophic marker strains
-
Sakai, Y., and Y. Tani. 1992. Directed mutagenesis in an asporogenous methylotrophic yeast: cloning, sequencing, and one-step gene disruption of the 3-isopropylmalate dehydrogenase gene (LEU2) of Candida boidinii to derive doubly auxotrophic marker strains. J. Bacteriol. 174:5988-5993.
-
(1992)
J. Bacteriol.
, vol.174
, pp. 5988-5993
-
-
Sakai, Y.1
Tani, Y.2
-
19
-
-
0003903343
-
-
Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
-
Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
-
(1989)
Molecular Cloning: A Laboratory Manual, 2nd Ed.
-
-
Sambrook, J.1
Fritsch, E.F.2
Maniatis, T.3
-
20
-
-
0025089125
-
Transformation of Aspergillus parasiticus with a homologous gene (pyrG) involved in pyrimidine biosynthesis
-
Skory, C. D., J. S. Horng, J. J. Pestka, and J. E. Linz. 1990. Transformation of Aspergillus parasiticus with a homologous gene (pyrG) involved in pyrimidine biosynthesis. Appl. Environ. Microbiol. 56:3315-3320.
-
(1990)
Appl. Environ. Microbiol.
, vol.56
, pp. 3315-3320
-
-
Skory, C.D.1
Horng, J.S.2
Pestka, J.J.3
Linz, J.E.4
-
21
-
-
0027202734
-
Recombination-deficient mutants of an extreme thermophile, Thermus thermophilus
-
Takada, T., S. Akanuma, T. Kotsuka, M. Tamakoshi, A. Yamagishi, and T. Oshima. 1993. Recombination-deficient mutants of an extreme thermophile, Thermus thermophilus. Appl. Environ. Microbiol. 59:2737-2739.
-
(1993)
Appl. Environ. Microbiol.
, vol.59
, pp. 2737-2739
-
-
Takada, T.1
Akanuma, S.2
Kotsuka, T.3
Tamakoshi, M.4
Yamagishi, A.5
Oshima, T.6
-
22
-
-
0029154808
-
Screening of stable proteins in an extreme thermophile, Thermus thermophilus
-
Tamakoshi, M., A. Yamagishi, and T. Oshima. 1995. Screening of stable proteins in an extreme thermophile, Thermus thermophilus. Mol. Microbiol. 16:1031-1036.
-
(1995)
Mol. Microbiol.
, vol.16
, pp. 1031-1036
-
-
Tamakoshi, M.1
Yamagishi, A.2
Oshima, T.3
-
23
-
-
0019510613
-
Cloning of 3-isopropylmalate dehydrogenase gene of an extreme thermophile and partial purification of the gene product
-
Tokyo
-
Tanaka, T., N. Kawano, and T. Oshima. 1981. Cloning of 3-isopropylmalate dehydrogenase gene of an extreme thermophile and partial purification of the gene product. J. Biochem. (Tokyo) 89:677-682.
-
(1981)
J. Biochem.
, vol.89
, pp. 677-682
-
-
Tanaka, T.1
Kawano, N.2
Oshima, T.3
-
24
-
-
0026014904
-
Production and extracellular secretion of aqualysin I (a thermophilic subtilisin-type protease) in a host-vector system for Thermus thermophilus
-
Touhara, N., H. Taguchi, Y. Koyama, T. Ohta, and H. Matsuzawa. 1991. Production and extracellular secretion of aqualysin I (a thermophilic subtilisin-type protease) in a host-vector system for Thermus thermophilus. Appl. Environ. Microbiol. 57:3385-3387.
-
(1991)
Appl. Environ. Microbiol.
, vol.57
, pp. 3385-3387
-
-
Touhara, N.1
Taguchi, H.2
Koyama, Y.3
Ohta, T.4
Matsuzawa, H.5
-
25
-
-
0026709492
-
Selection of a thermostable variant of chloramphenicol acetyltransferase (Cat-86)
-
Turner, S. L., G. C. Ford, A. Mountain, and A. Moir. 1992. Selection of a thermostable variant of chloramphenicol acetyltransferase (Cat-86). Protein Eng. 5:535-541.
-
(1992)
Protein Eng.
, vol.5
, pp. 535-541
-
-
Turner, S.L.1
Ford, G.C.2
Mountain, A.3
Moir, A.4
-
26
-
-
0028938565
-
A chromosome integration system for stable gene transfer into Thermus flavus
-
Weber, J. M., S. P. Johnson, V. Vonstein, M. J. Casadaban, and D. C. Demirjian. 1995. A chromosome integration system for stable gene transfer into Thermus flavus. Bio/Technology 13:271-275.
-
(1995)
Bio/Technology
, vol.13
, pp. 271-275
-
-
Weber, J.M.1
Johnson, S.P.2
Vonstein, V.3
Casadaban, M.J.4
Demirjian, D.C.5
-
27
-
-
0030001222
-
Pyrimidine biosynthesis genes (pyrE and pyrF) of an extreme thermophile, Thermus thermophilus
-
Yamagishi, A., T. Tanimoto, T. Suzuki, and T. Oshima. 1996. Pyrimidine biosynthesis genes (pyrE and pyrF) of an extreme thermophile, Thermus thermophilus. Appl. Environ. Microbiol. 62:2191-2194.
-
(1996)
Appl. Environ. Microbiol.
, vol.62
, pp. 2191-2194
-
-
Yamagishi, A.1
Tanimoto, T.2
Suzuki, T.3
Oshima, T.4
|