Further improvement of the thermal stability of a partially stabilized Bacillus subtilis 3-isopropylmalate dehydrogenase variant by random and site- directed mutagenesis

European Journal of Biochemistry

Volumn 260, Issue 2, 1999, Pages 499-504

  Akanuma, Satoshi ^a,b   Yamagishi, Akihiko ^a   Tanaka, Nobuo ^b   Oshima, Tairo ^a  

^a TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

^b TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

3 isopropylmalate dehydrogenase; Random mutagenesis; Site directed mutagenesis; Thermal stability; Thermus thermophilus

Indexed keywords

3 ISOPROPYLMALATE DEHYDROGENASE;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS SUBTILIS; ENZYME ACTIVITY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN STABILITY; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

3-ISOPROPYLMALATE DEHYDROGENASE; ALCOHOL OXIDOREDUCTASES; AMINO ACID SEQUENCE; BACILLUS SUBTILIS; ENZYME STABILITY; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN CONFORMATION; SEQUENCE ALIGNMENT; THERMUS THERMOPHILUS;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); THERMUS THERMOPHILUS;

EID: 0033106205     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00182.x     Document Type: Article

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