Spontaneous tandem sequence duplications reverse the thermal stability of carboxyl-terminal modified 3-isopropylmalate dehydrogenase

Journal of Bacteriology

Volumn 178, Issue 21, 1996, Pages 6300-6304

  Akanuma, Satoshi ^a,b   Yamagishi, Akihiko ^b   Tanaka, Nobuo ^a   Oshima, Tairo ^b  

^a TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^b TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

3 ISOPROPYLMALATE DEHYDROGENASE;

ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME STRUCTURE; EVOLUTION; GENE DELETION; HYDROPHOBICITY; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN ANALYSIS; TANDEM REPEAT; TEMPERATURE SENSITIVITY; THERMOSTABILITY; THERMUS THERMOPHILUS;

THERMUS THERMOPHILUS;

EID: 0029908683     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.21.6300-6304.1996     Document Type: Article

References (19)

1. Akanuma, S., A. Yamagishi, and T. Oshima. 1995. Thermostabilization of 3-isopropylmalate dehydrogenase from an extreme thermophile, Thermus thermophilus, by introducing a bulky side chain at position 172. Protein Eng. 8(Suppl.):4.
2. Hall, B. G. 1981. Changes in the substrate specificities of an enzyme during directed evolution of new functions. Biochemistry 20:4042-4049.
3. Imada, K., M. Sato, N. Tanaka, Y. Katsube, Y. Matsuura, and T. Oshima. 1991. Three-dimensional structure of a highly thermostable enzyme, 3-iso-propylmalate dehydrogenase of Thermus thermophilus at 2.2 Å resolution. J. Mol. Biol. 222:725-738.
4. Kagawa, Y., H. Nojima, N. Nukiwa, M. Ishizuka, T. Nakajima, T. Yasuhara, T. Tanaka, and T. Oshima. 1984. High guanine plus cytosine content in the third letter of codons of an extreme thermophile. J. Biol. Chem. 259:2956-2960.
5. Kirino, H., M. Aoki, M. Aoshima, Y. Hayashi, M. Ohba, A. Yamagishi, T. Wakagi, and T. Oshima. 1994. Hydrophobic interaction at the subunit interface contributes to the thermal stability of 3-isopropylmalate dehydrogenase from an extreme thermophile, Thermus thermophilus. Eur. J. Biochem. 220:275-281.
6. Kless, H., and W. Vermaas. 1995. Tandem sequence duplications functionally complement deletions in the D1 protein of photosystem II. J. Biol. Chem. 270:16536-16541.
7. Kotsuka, T., S. Akanuma, M. Tomuro, A. Yamagishi, and T. Oshima. 1996. Further stabilization of 3-isopropylmalate dehydrogenase of an extreme thermophile, Thermus thermophilus, by a suppressor mutation method. J. Bacteriol. 178:723-727.
8. Koyama, Y., T. Hoshino, N. Tomizuka, and K. Furukawa. 1986. Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp. J. Bacteriol. 166:338-340.
9. Kunkel, T. A., J. D. Roberts, and R. A. Zakour. 1987. Rapid and efficient site-specific mutagenesis without phenotype selection. Methods Enzymol. 154:367-382.
10. Liao, H., T. McKenzie, and R. Hageman. 1986. Isolation of a thermostable enzyme variant by cloning and selection in a thermophile. Proc. Natl. Acad. Sci. USA 83:576-580.
11. Numata, K., M. Muro, N. Akutsu, Y. Nosoh, A. Yamagishi, and T. Oshima. 1995. Thermal stability of chimeric isopropylmalate dehydrogenase genes constructed from a thermophile and a mesophile. Protein Eng. 8:39-43.
12. Oshima, T., and K. Imahori. 1974. Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from a Japanese thermal spa. Int. J. Syst. Bacteriol. 24:102-112.
13. Sakaki, Y., and T. Oshima. 1975. Isolation and characterization of a bacte riophage infectious to an extreme thermophile, Thermus thermophilus HB8. J. Virol. 15:1449-1453.
14. 13a.Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
15. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
16. Takada, T., S. Akanuma, T. Kotsuka, M. Tamakoshi, A. Yamagishi, and T. Oshima. 1993. Recombination-deficient mutants of an extreme thermophile, Thermus thermophilus. Appl. Environ. Microbiol. 59:2737-2739.
17. Tamakoshi, M., A. Yamagishi, and T. Oshima. 1995. Screening of stable proteins in an extreme thermophile, Thermus thermophilus. Mol. Microbiol. 16:1031-1036.
18. Tanaka, T., N. Kawano, and T. Oshima. 1981. Cloning of 3-isopropylmalate dehydrogenase gene of an extreme thermophile and partial purification of the gene product. J. Biochcm. 89:677-682.
19. Yamada, T., N. Akutsu, K. Miyazaki, K. Kakinuma, M. Yoshida, and T. Oshima. 1990. Purification, catalytic properties, and thermal stability of threo-Ds-3-isopropylmalate dehydrogenase coded by leuB gene from an extreme thermophile, Thermus thermophilus strain HB8. J. Biochem. 108:449-456.