Expression and purification of enzymatically active recombinant RNA-dependent RNA polymerase (NS5) of the flavivirus Kunjin

Journal of Virological Methods

Volumn 92, Issue 1, 2001, Pages 37-44

  Guyatt, Kimberley J ^a,b   Westaway, Edwin G ^a,b   Khromykh, Alexander A ^a,b  

^a ROYAL CHILDREN'S HOSPITAL   (Australia)

^b UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Flaviviruses; Recombinant baculovirus expression; RNA dependent RNA polymerase

Indexed keywords

RECOMBINANT ENZYME; RECOMBINANT RNA; RIBONUCLEASE A; RNA POLYMERASE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BACULOVIRUS; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PURIFICATION; FLAVIVIRUS; IN VITRO STUDY; KUNJIN VIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; VERO CELL; VIRUS REPLICATION;

ANIMALS; BACULOVIRIDAE; BINDING SITES; CERCOPITHECUS AETHIOPS; CHROMATOGRAPHY, AFFINITY; FLAVIVIRUS; GENETIC VECTORS; MUTATION; RECOMBINANT PROTEINS; RIBONUCLEASES; RNA REPLICASE; VERO CELLS;

EID: 0035150449     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(00)00270-6     Document Type: Article

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