Subgenomic replicons of the flavivirus Kunjin: Construction and applications

Journal of Virology

Volumn 71, Issue 2, 1997, Pages 1497-1505

  Khromykh, Alexander A ^a   Westaway, Edwin G ^a  

^a ROYAL CHILDREN'S HOSPITAL   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CARBOXY TERMINAL SEQUENCE; ENCEPHALOMYOCARDITIS VIRUS; FLAVIVIRUS; GENETIC CODE; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RNA REPLICATION; VIRUS EXPRESSION; VIRUS REPLICATION;

EID: 0031014022     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.71.2.1497-1505.1997     Document Type: Article

References (43)

1. Andino, R., G. E. Rieckhof, P. L. Achacoso, and D. Baltimore. 1993. Poliovirus RNA synthesis utilizes an RNP complex formed around the 5′-end of viral RNA. EMBO J. 12:3587-3598.
2. Ansardi, D. C., D. C. Porter, and C. D. Morrow. 1993. Complementation of a poliovirus defective genome by a recombinant vaccinia virus which provides poliovirus P1 capsid precursor in trans. J. Virol. 67:3684-3690.
3. Bredenbeek, P., and C. M. Rice. Personal communication.
4. Bredenbeek, P., and C. M. Rice. 1992. Animal RNA virus expression systems. Semin. Virol. 3:297-310.
5. Bredenbeek, P., I. Frolov, C. M. Rice, and S. Schlesinger. 1993. Sindbis virus expression vectors: packaging of RNA replicons by using defective helper RNAs. J. Virol. 67:6439-6446.
6. Cassinotti, P., and M. Weitz. 1994. Increasing the sensitivity of a common CAT assay. BioTechniques 17:36-40.
7. Choi, W. S., R. Pal-Ghosh, and C. D. Morrow. 1991. Expression of human immunodeficiency virus type 1 (HIV-1) Gag, Pol, and envelope proteins from chimeric HIV-1-poliovirus minireplicons. J. Virol. 65:2875-2883.
8. Chu, P. W. G., and E. G. Westaway. 1985. Replication strategy of Kunjin virus: evidence for recycling role of replicative form RNA as template in semiconservative and asymmetric replication, Virology 140:68-79.
9. Chu, P. W. G., and E. G. Westaway. 1987. Characterization of Kunjin virus RNA-dependent RNA polymerase: reinitiation of synthesis in vitro. Virology 157:330-337.
10. Chu, P. W. G., and E. G. Westaway. 1992. Molecular and ultrastructural analysis of heavy membrane fractions associated with the replication of Kunjin virus RNA. Arch. Virol. 125:177-191.
11. Chu, P. W. G., E. G. Westaway, and G. Coia. 1992. Comparison of centrifugation methods for molecular and morphological analysis of membranes associated with RNA replication of the flavivirus Kunjin. J. Virol. Methods 37:219-234.
12. Coia, G., M. D. Parker, G. Speight, M. E. Byrne, and E. G. Westaway. 1988. Nucleotide and complete amino acid sequences of Kunjin virus: definitive gene order and characteristics of the virus-specified proteins. J. Gen. Virol. 69:1-21.
13. Collis, P. S., B. J. O'Donnell, D. J. Barton, J. A. Rogers, and J. B. Flanegan. 1992. Replication of poliovirus RNA and subgenomic RNA transcripts in transfected cells. J. Virol. 66:6480-6488.
14. Frolov, I., and S. Schlesinger. 1994. Comparison of the effects of Sindbis virus and Sindbis virus replicons on host cell protein synthesis and cytopathogenicity in BHK cells. J. Virol. 68:1721-1727.
15. Frolov, I., and S. Schlesinger. 1994. Translation of Sindbis virus mRNA: effects of sequences downstream of the initiation codon. J. Virol. 68:8111-8117.
16. Hagino-Yamagishi, K., and A. Nomoto. 1989. In vitro construction of poliovirus defective interfering particles. J. Virol. 63:5386-5392.
17. Hahn, C. S., Y. S. Hahn, C. M. Rice, E. Lee, L. Dalgarno, E. G. Strauss, and J. H. Strauss. 1987. Conserved elements in the 3′ untranslated region of flavivirus RNAs and potential cyclization sequences. J. Mol. Biol. 198:33-41.
18. Johanning, F. W., R. M. Conry, A. F. LoBuglio, M. Wright, L. A. Sumerel, M. J. Pike, and D. T. Curiel. 1995. A Sindbis virus mRNA polynucleotide vector achieves prolonged and high level heterologous gene expression in vivo. Nucleic Acids Res. 23:1495-1501.
19. Kaplan, G., and V. R. Racaniello. 1988. Construction and characterization of poliovirus subgenomic replicons. J. Virol. 62:1687-1696.
20. Khromykh, A. A., and E. G. Westaway. Unpublished data.
21. Khromykh, A. A., and E. G. Westaway. 1994. Completion of Kunjin virus RNA sequence and recovery of an infectious RNA transcribed from stably cloned full-length cDNA. J. Virol. 68:4580-4588.
22. Khromykh, A. A., and E. G. Westaway. 1996. RNA binding properties of core protein of the flavivirus Kunjin. Arch. Virol. 141:685-699.
23. Khromykh, A. A., H. Meka, and E. G. Westaway. 1995. Preparation of recombinant baculovirus by transfection of a ligated cDNA fragment without prior plasmid amplification in E. coli. BioTechniques 19:356-360.
24. Khromykh, A. A., T. J. Harvey, M. Abedinia, and E. G. Westaway. 1996. Expression and purification of the seven nonstructural proteins of the flavivirus Kunjin in the E. coli and the baculovirus expression systems. J. Virol. Methods 61:47-58.
25. Leahy, P., G. G. Carmichael, and E. F. Rossomando. 1995. Effects of ethanol concentration and incubation period at 65°C on CAT activity in mammalian cell extracts. BioTechniques 19:894-898.
26. Liljestrom, P., and H. Garoff. 1991. A new generation of animal cell expression vectors based on the Semliki Forest virus replicon. Bio/Technology 9:1356-1361.
27. Liljestrom, R., S. Lusa, D. Huylebroeck, and H. Garoff. 1991. In vitro mutagenesis of a full-length cDNA clone of Semliki Forest virus: small 6,000-molecular-weight membrane protein modulates virus release. J. Virol. 65: 4107-4113.
28. McKnight, K., and S. M. Lemon. 1996. Capsid protein sequence is required for efficient replication of human rhinovirus 14 RNA. J. Virol. 70:1941-1952.
29. Men, R., M. Bray, D. Clark, R. Chanock, and C-J. Lai. 1996. Dengue type 4 virus mutants containing deletions in the 3′ noncoding region of the RNA genome: analysis of growth restriction in cell culture and altered viremia pattern and immunogenicity in rhesus monkeys. J. Virol. 70:3930-3937.
30. Parrish, C. R., G. Coia, A. Hill, A. Müllbacher, E. G. Westaway, and R. V. Blanden. 1991. Preliminary analysis of murine cytotoxic T cell responses to the proteins of the flavivirus Kunjin using vaccinia virus expression. J. Gen. Virol. 72:1645-1653.
31. Percy, N., W. S. Barclay, M. Sullivan, and J. W. Almond. 1992. A poliovirus replicon containing the chloramphenicol acetyltransferase gene can be used to study the replication and encapsidation of poliovirus RNA. J. Virol. 66:5040-5046.
32. Poidinger, M., R. Hall, and J. S. Mackenzie. 1996. Molecular characterization of the Japanese encephalitis serocomplex of the flavivirus genus. Virology 218:417-121.
33. Porter, D. C., D. C. Ansardi, W. S. Choi, and C. D. Morrow. 1993. Encapsidation of genetically engineered poliovirus minireplicons which express human immunodeficiency virus type 1 Gag and Pol proteins upon infection. J. Virol. 67:3712-3719.
34. Porter, D. C., D. C. Ansardi, and C. D. Morrow. 1995. Encapsidation of poliovirus replicons encoding the complete human immunodeficiency virus type 1 gag gene by using a complementation system which provides the P1 capsid protein in trans. J. Virol. 69:1548-1555.
35. Rees, S. J. Coote, J. Stablles, G. Goodson, S. Harris, and M. G. Lee. 1996. Bicistronic vector for the creation of stable mammalian cell lines that predisposes all antibiotic-resistant cells to express recombinant protein. Bio-Techniques 20:102-110.
36. Schlesinger, S. 1995. Alphaviruses-vectors for the expression of heterologous genes. Trends Biotechnol. 11:18-22.
37. Sjöberg, E. M., M. Suomalainen, and H. Garoff. 1994. A significantly improved Semliki Forest virus expression system based on translation enhancer segments from the viral capsid gene. Bio/Technology 12:1127-1131.
38. Southern, P. G., and P. Berg. 1982. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under the control of the SV40 early region promoter. J. Mol. Appl. Genet. 1:327-341.
39. Speight, G., and E. G. Westaway. 1989. Carboxy-terminal analysis of nine proteins specified by the flavivirus Kunjin: evidence that only the intracellular core protein is truncated. J. Gen. Virol. 70:2209-2214.
40. Wallner, G., C. W. Mandl, C. Kunz, and F. X. Heinz. 1995. The flavivirus 3′-noncoding region: extensive size heterogeneity independent of evolutionary relationships among strains of tick-borne encephalitis virus. Virology 213:169-178.
41. Westaway, E. G. 1987. Flavivirus replication strategy. Adv. Virus Res. 33: 45-90.
42. Whetter, L. A., S. P. Day, O. Elroy-Stein, E. A. Brown, and S. M. Lemon. 1994. Low efficiency of the 5′ nontranslated region of hepatitis A virus RNA in directing cap-independent translation in monkey kidney cells. J. Virol. 68:5253-5263.
43. Xiong, C., R. Levis, P. Shen, S. Schlesinger, C. M. Rice, and H. V. Huang. 1989. Sindbis virus: an efficient, broad host range vector for gene expression in animal cells. Science 243:1188-1191.