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Volumn 245, Issue 2, 1998, Pages 203-215

Subcellular localization and some biochemical properties of the flavivirus Kunjin nonstructural proteins NS2A and NS4A

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS PROTEIN;

EID: 0032486487     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1006/viro.1998.9156     Document Type: Article
Times cited : (269)

References (50)
  • 1
    • 0030761459 scopus 로고    scopus 로고
    • Translation elongation factor-1 alpha interacts with the 3′ stem-loop region of West Nile virus genomic RNA
    • Blackwell J. L., Brinton M. A. Translation elongation factor-1 alpha interacts with the 3′ stem-loop region of West Nile virus genomic RNA. J. Virol. 71:1997;6433-6444.
    • (1997) J. Virol. , vol.71 , pp. 6433-6444
    • Blackwell, J.L.1    Brinton, M.A.2
  • 2
    • 0029140676 scopus 로고
    • A novel complex formed between the flavivirus E and NS1 proteins: Analysis of its structure and function
    • Blitvich B. J., Mackenzie J. S., Coelen R. J., Howard M. J., Hall R. A. A novel complex formed between the flavivirus E and NS1 proteins: Analysis of its structure and function. Arch. Virol. 140:1995;145-156.
    • (1995) Arch. Virol. , vol.140 , pp. 145-156
    • Blitvich, B.J.1    MacKenzie, J.S.2    Coelen, R.J.3    Howard, M.J.4    Hall, R.A.5
  • 3
    • 0026540617 scopus 로고
    • Cleavage of the dengue virus polyprotein at the NS3/NS4A and NS4B/NS5 junction is mediated by viral proteinase NS2b-NS3, whereas NS4A/NS4B may be processed by a cellular protease
    • Cahour A., Falgout B., Lai C.-J. Cleavage of the dengue virus polyprotein at the NS3/NS4A and NS4B/NS5 junction is mediated by viral proteinase NS2b-NS3, whereas NS4A/NS4B may be processed by a cellular protease. J. Virol. 66:1992;1525-1542.
    • (1992) J. Virol. , vol.66 , pp. 1525-1542
    • Cahour, A.1    Falgout, B.2    Lai, C.-J.3
  • 4
    • 0024590191 scopus 로고
    • Yellow fever virus proteins NS2A, NS2B, and NS4B: Identification and partial N-terminal amino acid sequence analysis
    • Chambers T. J., McCourt D. W., Rice C. M. Yellow fever virus proteins NS2A, NS2B, and NS4B: Identification and partial N-terminal amino acid sequence analysis. Virology. 169:1989;100-109.
    • (1989) Virology , vol.169 , pp. 100-109
    • Chambers, T.J.1    McCourt, D.W.2    Rice, C.M.3
  • 5
    • 0026039916 scopus 로고
    • Processing of the yellow fever virus nonstructural polyprotein: A catalytically active NS3 proteinase domain and NS2B are required for cleavages at dibasic sites
    • Chambers T. J., Grakoui A., Rice C. M. Processing of the yellow fever virus nonstructural polyprotein: a catalytically active NS3 proteinase domain and NS2B are required for cleavages at dibasic sites. J. Virol. 65:1991;6042-6050.
    • (1991) J. Virol. , vol.65 , pp. 6042-6050
    • Chambers, T.J.1    Grakoui, A.2    Rice, C.M.3
  • 6
    • 0031001919 scopus 로고    scopus 로고
    • RNA-protein interactions: Involvement of NS3, NS5, and 3′ noncoding regions of Japanese encephalitis virus genomic RNA
    • Chen C.-J., Kuo M.-D., Chien L.-J., Hsu S.-L., Wang Y.-M., Lin J.-H. RNA-protein interactions: Involvement of NS3, NS5, and 3′ noncoding regions of Japanese encephalitis virus genomic RNA. J. Virol. 71:1997;3466-3473.
    • (1997) J. Virol. , vol.71 , pp. 3466-3473
    • Chen, C.-J.1    Kuo, M.-D.2    Chien, L.-J.3    Hsu, S.-L.4    Wang, Y.-M.5    Lin, J.-H.6
  • 7
    • 0021893927 scopus 로고
    • Replication strategy of Kunjin virus: Evidence for recycling role of replicative form RNA as a template in semiconservative and asymmetric replication
    • Chu P. W. G., Westaway E. G. Replication strategy of Kunjin virus: Evidence for recycling role of replicative form RNA as a template in semiconservative and asymmetric replication. Virology. 140:1985;68-79.
    • (1985) Virology , vol.140 , pp. 68-79
    • Chu, P.W.G.1    Westaway, E.G.2
  • 8
    • 0023101499 scopus 로고
    • Characterization of Kunjin virus RNA-dependent RNA polymerase: Reinitiation of synthesisin vitro.
    • Chu P. W. G., Westaway E. G. Characterization of Kunjin virus RNA-dependent RNA polymerase: Reinitiation of synthesisin vitro. Virology. 157:1987;330-337.
    • (1987) Virology , vol.157 , pp. 330-337
    • Chu, P.W.G.1    Westaway, E.G.2
  • 9
    • 0026494350 scopus 로고
    • Molecular and ultrastructural analysis of heavy membrane fractions associated with the replication of Kunjin virus RNA
    • Chu P. W. G., Westaway E. G. Molecular and ultrastructural analysis of heavy membrane fractions associated with the replication of Kunjin virus RNA. Arch. Virol. 125:1992;177-191.
    • (1992) Arch. Virol. , vol.125 , pp. 177-191
    • Chu, P.W.G.1    Westaway, E.G.2
  • 10
    • 0026590169 scopus 로고
    • Comparison of centrifugation methods for molecular and morphological analysis of membranes associated with RNA replication of the flavivirus Kunjin
    • Chu P. W. G., Westaway E. G., Coia G. Comparison of centrifugation methods for molecular and morphological analysis of membranes associated with RNA replication of the flavivirus Kunjin. J. Virol. Methods. 37:1992;219-234.
    • (1992) J. Virol. Methods , vol.37 , pp. 219-234
    • Chu, P.W.G.1    Westaway, E.G.2    Coia, G.3
  • 11
    • 0023854242 scopus 로고
    • Nucleotide and complete amino acid sequences of Kunjin virus: Definitive gene order and characteristics of the virus-specified proteins
    • Coia G., Parker M. D., Speight G., Byrne M. E., Westaway E. G. Nucleotide and complete amino acid sequences of Kunjin virus: Definitive gene order and characteristics of the virus-specified proteins. J. Gen. Virol. 69:1988;1-21.
    • (1988) J. Gen. Virol. , vol.69 , pp. 1-21
    • Coia, G.1    Parker, M.D.2    Speight, G.3    Byrne, M.E.4    Westaway, E.G.5
  • 12
    • 0027154255 scopus 로고
    • Localization and functions of Japanese encephalitis virus nonstructural proteins NS3 and NS5 for viral RNA synthesis in the infected cell
    • Edward Z., Takegami T. Localization and functions of Japanese encephalitis virus nonstructural proteins NS3 and NS5 for viral RNA synthesis in the infected cell. Microbiol. Immunol. 37:1993;239-243.
    • (1993) Microbiol. Immunol. , vol.37 , pp. 239-243
    • Edward, Z.1    Takegami, T.2
  • 13
    • 0028849770 scopus 로고
    • Evidence that flavivirus NS1-NS2A cleavage is mediated by a membrane-bound host protease in the endoplasmic reticulum
    • Falgout B., Markoff L. Evidence that flavivirus NS1-NS2A cleavage is mediated by a membrane-bound host protease in the endoplasmic reticulum. J. Virol. 69:1995;7232-7243.
    • (1995) J. Virol. , vol.69 , pp. 7232-7243
    • Falgout, B.1    Markoff, L.2
  • 14
    • 0024553952 scopus 로고
    • Proper processing of dengue virus nonstructural glycoprotein NS1 requires the N-terminal hydrophobic signal sequence and the downstream nonstructural protein NS2a
    • Falgout B., Chanock R., Lai C.-J. Proper processing of dengue virus nonstructural glycoprotein NS1 requires the N-terminal hydrophobic signal sequence and the downstream nonstructural protein NS2a. J. Virol. 63:1989;1852-1860.
    • (1989) J. Virol. , vol.63 , pp. 1852-1860
    • Falgout, B.1    Chanock, R.2    Lai, C.-J.3
  • 15
    • 0025864149 scopus 로고
    • Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins
    • Falgout B., Pethel M., Zhang Y. M., Lai C.-J. Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins. J. Virol. 65:1991;2467-2475.
    • (1991) J. Virol. , vol.65 , pp. 2467-2475
    • Falgout, B.1    Pethel, M.2    Zhang, Y.M.3    Lai, C.-J.4
  • 16
    • 0024341494 scopus 로고
    • N-terminal domains of putative helicases of flavi- And pestiviruses may be serine proteases
    • Gorbalenya A. E., Donchenko A. P., Koonin E. V., Blinov V. M. N-terminal domains of putative helicases of flavi- and pestiviruses may be serine proteases. Nucleic Acids Res. 77:1989;3889-3897.
    • (1989) Nucleic Acids Res. , vol.77 , pp. 3889-3897
    • Gorbalenya, A.E.1    Donchenko, A.P.2    Koonin, E.V.3    Blinov, V.M.4
  • 17
    • 0031445610 scopus 로고    scopus 로고
    • Intracellular localisation of dengue-2 RNA in mosquito cell culture using electron microscopic in situ hybridisation
    • Grief C., Galler R., Côrtes L. M. C., Barth O. M. Intracellular localisation of dengue-2 RNA in mosquito cell culture using electron microscopic in situ hybridisation. Arch. Virol. 142:1997;2347-2357.
    • (1997) Arch. Virol. , vol.142 , pp. 2347-2357
    • Grief, C.1    Galler, R.2    Côrtes, L.M.C.3    Barth, O.M.4
  • 18
    • 0029027850 scopus 로고
    • Association between NS3 and NS5 proteins of dengue virus type 2 in the putative RNA replicase is linked to differential phosphorylation of NS5
    • Kapoor M., Zhang L., Ramachandra M., Kusukawa J., Ebner K. E., Padmanabhan R. Association between NS3 and NS5 proteins of dengue virus type 2 in the putative RNA replicase is linked to differential phosphorylation of NS5. J. Biol. Chem. 270:1995;19100-19106.
    • (1995) J. Biol. Chem. , vol.270 , pp. 19100-19106
    • Kapoor, M.1    Zhang, L.2    Ramachandra, M.3    Kusukawa, J.4    Ebner, K.E.5    Padmanabhan, R.6
  • 19
    • 0028337164 scopus 로고
    • Completion of Kunjin virus RNA sequence and recovery of an infectious RNA transcribed from stably cloned full-length cDNA
    • Khromykh A. A., Westaway E. G. Completion of Kunjin virus RNA sequence and recovery of an infectious RNA transcribed from stably cloned full-length cDNA. J. Virol. 68:1994;4580-4588.
    • (1994) J. Virol. , vol.68 , pp. 4580-4588
    • Khromykh, A.A.1    Westaway, E.G.2
  • 20
    • 0029684652 scopus 로고    scopus 로고
    • RNA binding properties of core protein of the flavivirus Kunjin
    • Khromykh A. A., Westaway E. G. RNA binding properties of core protein of the flavivirus Kunjin. Arch. Virol. 141:1996;685-699.
    • (1996) Arch. Virol. , vol.141 , pp. 685-699
    • Khromykh, A.A.1    Westaway, E.G.2
  • 21
    • 0031014022 scopus 로고    scopus 로고
    • Subgenomic replicons of the flavivirus Kunjin: Construction and applications
    • Khromykh A. A., Westaway E. G. Subgenomic replicons of the flavivirus Kunjin: Construction and applications. J. Virol. 71:1997;1497-1505.
    • (1997) J. Virol. , vol.71 , pp. 1497-1505
    • Khromykh, A.A.1    Westaway, E.G.2
  • 22
    • 0030249999 scopus 로고    scopus 로고
    • Expression and purification of the seven nonstructural proteins of the flavivirus Kunjin in theE. coli
    • Khromykh A. A., Harvey T. J., Abedinia M., Westaway E. G. Expression and purification of the seven nonstructural proteins of the flavivirus Kunjin in theE. coli. J. Virol. Methods. 61:1996;47-58.
    • (1996) J. Virol. Methods , vol.61 , pp. 47-58
    • Khromykh, A.A.1    Harvey, T.J.2    Abedinia, M.3    Westaway, E.G.4
  • 23
    • 0027474037 scopus 로고
    • Computer-assisted identification of a putative methyltransferase domain in NS5 protein of flaviviruses and lambda 2 protein of reovirus
    • Koonin E. V. Computer-assisted identification of a putative methyltransferase domain in NS5 protein of flaviviruses and lambda 2 protein of reovirus. J. Gen. Virol. 74:1993;733-740.
    • (1993) J. Gen. Virol. , vol.74 , pp. 733-740
    • Koonin, E.V.1
  • 24
    • 0019254073 scopus 로고
    • Sequential events in the morphogenesis of Japanese encephalitis virus
    • Leary K., Blair C. D. Sequential events in the morphogenesis of Japanese encephalitis virus. J. Ultrastruc. Res. 72:1980;123-129.
    • (1980) J. Ultrastruc. Res. , vol.72 , pp. 123-129
    • Leary, K.1    Blair, C.D.2
  • 25
    • 0028331985 scopus 로고
    • Characterization of rubella virus replication complexes using antibodies to double-stranded RNA
    • Lee J.-Y., Marshall J. A., Bowden D. S. Characterization of rubella virus replication complexes using antibodies to double-stranded RNA. Virology. 200:1994;307-312.
    • (1994) Virology , vol.200 , pp. 307-312
    • Lee, J.-Y.1    Marshall, J.A.2    Bowden, D.S.3
  • 26
    • 0027475018 scopus 로고
    • Cleavage at a novel site in the NS4A region by the yellow fever virus NS2B-3 proteinase is a prerequisite for processing at the downstream 4A/4B signalase site
    • Lin C., Amberg S. M., Chambers T. J., Rice C. M. Cleavage at a novel site in the NS4A region by the yellow fever virus NS2B-3 proteinase is a prerequisite for processing at the downstream 4A/4B signalase site. J. Virol. 67:1993;2327-2335.
    • (1993) J. Virol. , vol.67 , pp. 2327-2335
    • Lin, C.1    Amberg, S.M.2    Chambers, T.J.3    Rice, C.M.4
  • 28
    • 0026756141 scopus 로고
    • Proteolytic processing of a Murray Valley encephalitis virus non-structural polyprotein segment containing the viral proteinase: Accumulation of a NS3-4A precursor which requires mature NS3 for efficient processing
    • Lobigs M. Proteolytic processing of a Murray Valley encephalitis virus non-structural polyprotein segment containing the viral proteinase: Accumulation of a NS3-4A precursor which requires mature NS3 for efficient processing. J. Gen. Virol. 73:1992;2305-2312.
    • (1992) J. Gen. Virol. , vol.73 , pp. 2305-2312
    • Lobigs, M.1
  • 29
    • 0029941322 scopus 로고    scopus 로고
    • Immunolocalization of the dengue virus nonstructural glycoprotein NS1 suggests a role in viral RNA replication
    • Mackenzie J. M., Jones M. K., Young P. R. Immunolocalization of the dengue virus nonstructural glycoprotein NS1 suggests a role in viral RNA replication. Virology. 220:1996a;232-240.
    • (1996) Virology , vol.220 , pp. 232-240
    • MacKenzie, J.M.1    Jones, M.K.2    Young, P.R.3
  • 30
    • 0029923202 scopus 로고    scopus 로고
    • Improved membrane preservation of flavivirus-infected cells with cryosectioning
    • Mackenzie J. M., Jones M. K., Young P. R. Improved membrane preservation of flavivirus-infected cells with cryosectioning. J. Virol. Methods. 56:1996b;67-75.
    • (1996) J. Virol. Methods , vol.56 , pp. 67-75
    • MacKenzie, J.M.1    Jones, M.K.2    Young, P.R.3
  • 31
    • 0024502091 scopus 로고
    • Maturation of Japanese encephalitis virus glycoproteins produced by infected mamalian and mosquito cells
    • Mason P. W. Maturation of Japanese encephalitis virus glycoproteins produced by infected mamalian and mosquito cells. Virology. 169:1989;354-364.
    • (1989) Virology , vol.169 , pp. 354-364
    • Mason, P.W.1
  • 32
    • 0023278851 scopus 로고
    • Expression of Japanese encephalitis virus antigens inEscherichia coli.
    • Mason P. W., McAda P. C., Dalrymple J. M., Fournier M. J., Mason T. C. Expression of Japanese encephalitis virus antigens inEscherichia coli. Virology. 158:1987;361-372.
    • (1987) Virology , vol.158 , pp. 361-372
    • Mason, P.W.1    McAda, P.C.2    Dalrymple, J.M.3    Fournier, M.J.4    Mason, T.C.5
  • 34
    • 0024406245 scopus 로고
    • Flavivirus infection: Essential ultrastructural changes and association of Kunjin virus NS3 protein with microtubules
    • Ng M. L., Hong S. S. Flavivirus infection: Essential ultrastructural changes and association of Kunjin virus NS3 protein with microtubules. Arch. Virol. 106:1989;103-120.
    • (1989) Arch. Virol. , vol.106 , pp. 103-120
    • Ng, M.L.1    Hong, S.S.2
  • 35
    • 0028019860 scopus 로고
    • Cryosubstitution technique reveals new morphology of flavivirus-induced structures
    • Ng M. L., Yeong F. M., Tan S. H. Cryosubstitution technique reveals new morphology of flavivirus-induced structures. J. Virol. Methods. 49:1994;305-314.
    • (1994) J. Virol. Methods , vol.49 , pp. 305-314
    • Ng, M.L.1    Yeong, F.M.2    Tan, S.H.3
  • 36
    • 0025033393 scopus 로고
    • In vitro processing of dengue virus type 2 nonstructural proteins NS2A, NS2B, and NS3
    • Preugschat F., Yao C. W., Strauss J. H. In vitro processing of dengue virus type 2 nonstructural proteins NS2A, NS2B, and NS3. J. Virol. 64:1990;4364-4374.
    • (1990) J. Virol. , vol.64 , pp. 4364-4374
    • Preugschat, F.1    Yao, C.W.2    Strauss, J.H.3
  • 37
    • 0021863828 scopus 로고
    • Nucleotide sequence of yellow fever virus: Implications for flavivirus gene expression and evolution
    • Rice C. M., Lenches E. M., Eddy S. R., Shin S. J., Sheets R. L., Strauss J. H. Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution. Science. 229:1985;726-733.
    • (1985) Science , vol.229 , pp. 726-733
    • Rice, C.M.1    Lenches, E.M.2    Eddy, S.R.3    Shin, S.J.4    Sheets, R.L.5    Strauss, J.H.6
  • 38
    • 0024517668 scopus 로고
    • Positive identification of NS4A, the last of the hypothetical nonstructural proteins of flaviviruses
    • Speight G., Westaway E. G. Positive identification of NS4A, the last of the hypothetical nonstructural proteins of flaviviruses. Virology. 170:1989a;299-301.
    • (1989) Virology , vol.170 , pp. 299-301
    • Speight, G.1    Westaway, E.G.2
  • 39
    • 0024343113 scopus 로고
    • Carboxy-terminal analysis of nine proteins specified by the flavivirus Kunjin: Evidence that only the intracellular core protein is truncated
    • Speight G., Westaway E. G. Carboxy-terminal analysis of nine proteins specified by the flavivirus Kunjin: Evidence that only the intracellular core protein is truncated. J. Gen. Virol. 70:1989b;2209-2214.
    • (1989) J. Gen. Virol. , vol.70 , pp. 2209-2214
    • Speight, G.1    Westaway, E.G.2
  • 40
    • 0023836729 scopus 로고
    • Gene mapping and positive identification of the nonstructural proteins NS2A, NS2B, NS3, NS4B and NS5 of the flavivirus Kunjin and their cleavage sites
    • Speight G., Coia G., Parker M. D., Westaway E. G. Gene mapping and positive identification of the nonstructural proteins NS2A, NS2B, NS3, NS4B and NS5 of the flavivirus Kunjin and their cleavage sites. J. Gen. Virol. 69:1988;23-34.
    • (1988) J. Gen. Virol. , vol.69 , pp. 23-34
    • Speight, G.1    Coia, G.2    Parker, M.D.3    Westaway, E.G.4
  • 41
  • 43
    • 0029863798 scopus 로고    scopus 로고
    • Recombinant dengue type 1 virus NS5 protein expressed inEscherichia coli
    • Tan B.-H., Fu J., Sugrue R. J., Yap E.-H., Chan Y.-C., Tan Y. H. Recombinant dengue type 1 virus NS5 protein expressed inEscherichia coli. Virology. 216:1996;317-325.
    • (1996) Virology , vol.216 , pp. 317-325
    • Tan, B.-H.1    Fu, J.2    Sugrue, R.J.3    Yap, E.-H.4    Chan, Y.-C.5    Tan, Y.H.6
  • 44
    • 0025945616 scopus 로고
    • The carboxy-terminal part of the NS3 protein of the West Nile flavivirus can be isolated as a soluble protein after proteolytic cleavage and represents an RNA-stimulated NTPase
    • Wengler G., Wengler G. The carboxy-terminal part of the NS3 protein of the West Nile flavivirus can be isolated as a soluble protein after proteolytic cleavage and represents an RNA-stimulated NTPase. Virology. 184:1991;707-715.
    • (1991) Virology , vol.184 , pp. 707-715
    • Wengler, G.1    Wengler, G.2
  • 45
    • 0025356409 scopus 로고
    • Description of a procedure which allows isolation of viral nonstructural proteins from BHK vertebrate cells infected with the West Nile flavivirus in a state which allows their direct chemical charaterization
    • Wengler G., Wengler G., Nowak T., Castle E. Description of a procedure which allows isolation of viral nonstructural proteins from BHK vertebrate cells infected with the West Nile flavivirus in a state which allows their direct chemical charaterization. Virology. 177:1990;795-801.
    • (1990) Virology , vol.177 , pp. 795-801
    • Wengler, G.1    Wengler, G.2    Nowak, T.3    Castle, E.4
  • 47
    • 0023062565 scopus 로고
    • Flavivirus replication strategy
    • Westaway E. G. Flavivirus replication strategy. Adv. Virus Res. 33:1987;45-90.
    • (1987) Adv. Virus Res. , vol.33 , pp. 45-90
    • Westaway, E.G.1
  • 48
    • 0031582633 scopus 로고    scopus 로고
    • Proteins C and NS4B of the flavivirus Kunjin translocate independently into the nucleus
    • Westaway E. G., Khromykh A. A., Kenney M. T., Mackenzie J. M., Jones M. K. Proteins C and NS4B of the flavivirus Kunjin translocate independently into the nucleus. Virology. 234:1997a;31-41.
    • (1997) Virology , vol.234 , pp. 31-41
    • Westaway, E.G.1    Khromykh, A.A.2    Kenney, M.T.3    MacKenzie, J.M.4    Jones, M.K.5
  • 49
    • 0030846512 scopus 로고    scopus 로고
    • Ultrastructure of Kunjin virus-infected cells: Co-localization of NS1 and NS3 with double-stranded RNA, and of NS2B with NS3, in virus-induced membrane structures
    • Westaway E. G., Mackenzie J. M., Kenney M. T., Jones M. K., Khromykh A. A. Ultrastructure of Kunjin virus-infected cells: co-localization of NS1 and NS3 with double-stranded RNA, and of NS2B with NS3, in virus-induced membrane structures. J. Virol. 71:1997b;6650-6661.
    • (1997) J. Virol. , vol.71 , pp. 6650-6661
    • Westaway, E.G.1    MacKenzie, J.M.2    Kenney, M.T.3    Jones, M.K.4    Khromykh, A.A.5
  • 50
    • 0026471347 scopus 로고
    • Processing and localization of dengue virus type 2 polyprotein precursor NS3-NS4A-NS4B-NS5
    • Zhang L., Mohan P. M., Padmanabhan R. Processing and localization of dengue virus type 2 polyprotein precursor NS3-NS4A-NS4B-NS5. J. Virol. 66:1992;7549-7554.
    • (1992) J. Virol. , vol.66 , pp. 7549-7554
    • Zhang, L.1    Mohan, P.M.2    Padmanabhan, R.3


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