Green fluorescent protein (GFP) fusion constructs in gene therapy research

Histochemistry and Cell Biology

Volumn 115, Issue 1, 2001, Pages 59-65

  Wahlfors, Jarmo ^a   Loimas, Sami ^a   Pasanen, Tiina ^a   Hakkarainen, Tanja ^a  

^a UNIVERSITY OF EASTERN FINLAND   (Finland)

Author keywords

Gene therapy; GFP fusion protein

Indexed keywords

AQUAPORIN 1; COMPLEMENTARY RNA; CYTOCHROME P450 ISOENZYME; FAS LIGAND; GENE PRODUCT; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MEMBRANE PROTEIN; PROTEIN P53; PROTEIN VP22; RIBOZYME; THYMIDINE KINASE; TRANSMEMBRANE CONDUCTANCE REGULATOR; UNCLASSIFIED DRUG;

ARTICLE; CANCER; CELL; CHIMERA; CYSTIC FIBROSIS; GENE EXPRESSION; GENE THERAPY; GENE TRANSFER; HUMAN; MARKER GENE; NONHUMAN; PRIORITY JOURNAL; SALIVARY GLAND DISEASE;

EID: 0035102283     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004180000219     Document Type: Article

References (49)

1. Intercellular spread of GFP-VP22
2. Polyethylene glycol-mediated infection of non-permissive mammalian cells with Semliki Forest virus: Application to signal transduction studies
3. Evaluation of VP22 spread in tissue culture
4. Green fluorescent protein as a marker for gene expression
5. Differential intracellular compartmentalization of herpetic thymidine kinases (TKs) in TK gene-transfected tumor cells: Molecular characterization of the nuclear localization signal of herpes simplex virus type 1 TK
6. The N-terminal matrix domain of HIV-1 Gag is sufficient but not necessary for viral protein U-mediated enhancement of particle release through a membrane-targeting mechanism
7. Intercellular delivery of thymidine kinase prodrug activating enzyme by the herpes simplex virus protein, VP22
8. Intercellular trafficking and protein delivery by a herpesvirus structural protein
9. Intercellular trafficking of VP22-GFP fusion proteins
10. Live-cell analysis of a green fluorescent protein-tagged herpes simplex virus infection
11. Cytoplasm-to-nucleus translocation of a herpesvirus tegument protein during cell division
12. Intercellular trafficking of VP22-GFP fusion proteins is not observed in cultured mammalian cells
13. Pseudotyped retrovector encoding for GFP and HSV-tk fusion protein serves as a versatile suicide/reporter instrument for cell and gene therapy applications
14. Protein traps: Using intracellular localization for cloning
15. Co-localization of HIV-1 Nef with the AP-2 adaptor protein complex correlates with Nef-induced CD4 down-regulation
16. Green fluorescent protein retroviral vectors - Low titer and high recombination frequency suggest a selective disadvantage
17. Functional expression of exogenous proteins in mammalian sensory hair cells infected with adenoviral vectors
18. Construction and function of a recombinant adenovirus encoding a human aquaporin 1-green fluorescent protein fusion product
19. Recombinant AAV-2 harboring gfp-anti-sense/ribozyme fusion sequences monitor transduction, gene expression, and show anti-HIV-1 efficacy
20. Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein
21. Functional coexpression of HSV-1 thymidine kinase and green fluorescent protein: Implications for noninvasive imaging of transgene expression
22. Applications of green fluorescent protein as a marker of retroviral vectors
23. High-efficiency transfer of cystic fibrosis transmembrane conductance regulator cDNA into cystic fibrosis airway cells in culture using lactosylated polylysine as a vector
24. Is green fluorescent protein toxic to the living cells?
25. Herpes simplex virus thymi-dine kinase green fluorescent protein fusion gene: New tool for gene transfer studies and gene therapy
26. Green fluorescent protein as a reporter for macromolecular localization in bacterial cells
27. A method to identify cDNAs based on localization of green fluorescent protein fusion products
28. Genetic strategies to inhibit HIV
29. Suicide genes: Gene therapy applications using enzyme/prodrug strategies
30. GFP:HIV-1 protease production and packaging with a T4 phage expression-packaging processing system
31. A fluorescent P53GFP fusion protein facilitates its detection in mammalian cells while retaining the properties of wild-type p53
32. Localization, trafficking, and temperature-dependence of the Aequorea green fluorescent protein in cultured vertebrate cells
33. Control of the functional activity of an antisense RNA by a tetracycline-responsive derivative of the human U6 snRNA promoter
34. Imaging cells in the developing nervous system with retrovirus expressing modified green fluorescent protein
35. Intercellular delivery of functional p53 by the herpesvirus protein VP22
36. Nuclear localization of human immunodeficiency virus type 1 integrase expressed as a fusion protein with green fluorescent protein
37. Primary structure of the Aequorea victoria green-fluorescent protein
38. A chimeric fusion protein of cyto-chrome CYP4B1 and green fluorescent protein for detection of pro-drug activating gene delivery and for gene therapy in malignant glioma
39. Chimeric green fluorescent protein as a tool for visualizing subcellu-lar organelles in living cells
40. Double labelling of subcellular structures with organelle-targeted GFP mutants in vivo
41. Adenoviral vector which delivers FasL-GFP fusion protein regulated by the tet-inducible expression system
42. Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea
43. Intracellular trafficking and interactions of the HIV-1 Tat protein
44. Analysis of trafficking of Rev and transdominant Rev proteins in living cells using green fluorescent protein fusions: Transdominant Rev blocks the export of Rev from the nucleus to the cytoplasm
45. Enhanced green fluorescent protein fusion proteins of herpes simplex virus type 1 thymidine kinase and cytochrome P4504B1: Applications for prodrug-activating gene therapy
46. Lentiviral vectors: Turning a deadly foe into a therapeutic agent
47. The green fluorescent protein
48. Evaluation of recombinant alphaviruses as vectors in gene therapy
49. Quantification of VP22-GFP spread by direct fluorescence in 15 commonly used cell lines