The N-terminal matrix domain of HIV-1 Gag is sufficient but not necessary for viral protein U-mediated enhancement of particle release through a membrane-targeting mechanism

Virology

Volumn 269, Issue 2, 2000, Pages 305-312

  Deora, Aparna ^a   Spearman, Paul ^b,c   Ratner, Lee ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VANDERBILT UNIVERSITY   (United States)

^c VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

HIV 1 Gag; Matrix; Membrane; Vpu

Indexed keywords

GAG PROTEIN; HYBRID PROTEIN; VIRUS PROTEIN; P55 GAG PRECURSOR PROTEIN, HUMAN IMMUNODEFICIENCY VIRUS 1; PROTEIN PRECURSOR; VPU PROTEIN;

ARTICLE; CELL FRACTIONATION; CELL MEMBRANE; CELLULAR DISTRIBUTION; CONFOCAL MICROSCOPY; DELETION MUTANT; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; MEMBRANE BINDING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN LOCALIZATION; PROTEIN TARGETING; VIRUS ASSEMBLY; VIRUS PARTICLE; HELA CELL; METABOLISM; PHYSIOLOGY;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES;

CELL MEMBRANE; GENE PRODUCTS, GAG; GENE PRODUCTS, VPU; HELA CELLS; HIV-1; HUMANS; MICROSCOPY, CONFOCAL; PROTEIN PRECURSORS; RECOMBINANT FUSION PROTEINS;

EID: 0034629752     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1006/viro.1999.0094     Document Type: Article

References (39)

1. Bour S., Geleziunas R., Wainberg M. A. The human immunodeficiency virus type 1 (HIV-1) CD4 receptor and its central role in promotion of HIV-1 infection. Microbiol. Rev. 59:1995;63-93.
2. Chen B. K., Gandhi R. T., Baltimore D. CD4 down-modulation during infection of human T cells with human immunodeficiency virus type 1 involves independent activities of vpu, env, and nef. J. Virol. 70:1996;6044-6053.
3. Chen M. Y., Maldarelli F., Karczewski M. K., Willey R. L., Strebel K. Human immunodeficiency virus type 1 Vpu protein induces degradation of CD4 in vitro: The cytoplasmic domain of CD4 contributes to Vpu sensitivity. J. Virol. 67:1993;3877-3884.
4. Coady M. J., Daniel N. G., Tiganos E., Allain B., Friborg J., Lapointe J. Y., Cohen E. A. Effects of Vpu expression on Xenopus oocyte membrane conductance. Virology. 244:1998;39-49.
5. Cohen E. A., Terwilliger E. F., Sodroski J. G., Haseltine W. A. Identification of a protein encoded by the vpu gene of HIV-1. Nature. 334:1988;532-534.
6. Emerman M., Malim M. H. HIV-1 regulatory/accessory genes: Keys to unraveling viral and host cell biology. Science. 280:1998;1880-1884.
7. Ewart G. D., Sutherland T., Gage P. W., Cox G. B. The Vpu protein of human immunodeficiency virus type 1 forms cation-selective ion channels. J. Virol. 70:1996;7108-7115.
8. Garnier L., Bowzard J. B., Wills J. W. Recent advances and remaining problems in HIV assembly. AIDS. 12:1998;S5-S16.
9. Geraghty R. J., Panganiban A. T. Human immunodeficiency virus type 1 Vpu has a CD4- and an envelope glycoprotein-independent function. J. Virol. 67:1993;4190-4194.
10. Giddings A. M., Ritter G. D. Jr., Mulligan M. J. The matrix protein of HIV-1 is not sufficient for assembly and release of virus-like particles. Virology. 248:1998;108-116.
11. Gonzalez S. A., Affranchino J. L., Gelderblom H. R., Burny A. Assembly of the matrix protein of simian immunodeficiency virus into virus-like particles. Virology. 194:1993;548-556.
12. Gonzalez S. A., Burny A., Affranchino J. L. Identification of domains in the simian immunodeficiency virus matrix protein essential for assembly and envelope glycoprotein incorporation. J. Virol. 70:1996;6384-6389.
13. Gottlinger H. G., Dorfman T., Cohen E. A., Haseltine W. A. Vpu protein of human immunodeficiency virus type 1 enhances the release of capsids produced by gag gene constructs of widely divergent retroviruses. Proc. Natl. Acad. Sci. USA. 90:1993;7381-7385.
14. Jabbar M. A. The human immunodeficiency virus type 1 Vpu protein: roles in virus release and CD4 downregulation. Curr. Topics Microbiol. Immunol. 193:1995;107-120.
15. Kerkau T., Bacik I., Bennink J. R., Yewdell J. W., Hunig T., Schimpl A., Schubert U. The human immunodeficiency virus type 1 (HIV-1) Vpu protein interferes with an early step in the biosynthesis of major histocompatibility complex (MHC) class I molecules. J. Exp. Med. 185:1997;1295-1305.
16. Kobinger G. P., Mouland A. J., Lalonde J. P., Forget J., Cohen E. A. Enhancement of retroviral production from packaging cell lines expressing the human immunodeficiency type 1 VPU gene. Gene Ther. 4:1997;868-874.
17. Lee Y. H., Schwartz M. D., Panganiban A. T. The HIV-1 matrix domain of Gag is required for Vpu responsiveness during particle release. Virology. 237:1997;46-55.
18. Lenburg M. E., Landau N. R. Vpu-induced degradation of CD4: Requirement for specific amino acid residues in the cytoplasmic domain of CD4. J. Virol. 67:1993;7238-7245.
19. Margottin F., Benichou S., Durand H., Richard V., Liu L. X., Gomas E., Benarous R. Interaction between the cytoplasmic domains of HIV-1 Vpu and CD4: Role of Vpu residues involved in CD4 interaction and in vitro CD4 degradation. Virology. 223:1996;381-386.
20. Margottin F., Bour S. P., Durand H., Selig L., Benichou S., Richard V., Thomas D., Strebel K., Benarous R. A novel human WD protein, h-beta TrCp, that interacts with HIV-1 Vpu connects CD4 to the ER degradation pathway through an F-box motif. Mol. Cell. 1:1998;565-574.
21. Moore P. B., Zhong Q., Husslein T., Klein M. L. Simulation of the HIV-1 Vpu transmembrane domain as a pentameric bundle. FEBS Lett. 431:1998;143-148.
22. Morand J. N., Kent C. A one-step technique for the subcellular fractionation of total cell homogenates. Anal. Biochem. 159:1986;157-162.
23. Raja N. U., Vincent M. J., abdul Jabbar M. Vpu-mediated proteolysis of gp160/CD4 chimeric envelope glycoproteins in the endoplasmic reticulum: Requirement of both the anchor and cytoplasmic domains of CD4. Virology. 204:1994;357-366.
24. Sandefur S., Varthakavi V., Spearman P. The I domain is required for efficient plasma membrane binding of human immunodeficiency virus type 1 Pr55Gag. J. Virol. 72:1998;2723-2732.
25. Schubert U., Anton L. C., Bacik I., Cox J. H., Bour S., Bennink J. R., Orlowski M., Strebel K., Yewdell J. W. CD4 glycoprotein degradation induced by human immunodeficiency virus type 1 Vpu protein requires the function of proteasomes and the ubiquitin-conjugating pathway. J. Virol. 72:1998;2280-2288.
26. Schubert U., Bour S., Ferrer-Montiel A. V., Montal M., Maldarell F., Strebel K. The two biological activities of human immunodeficiency virus type 1 Vpu protein involve two separable structural domains. J. Virol. 70:1996a;809-819.
27. Schubert U., Clouse K. A., Strebel K. Augmentation of virus secretion by the human immunodeficiency virus type 1 Vpu protein is cell type independent and occurs in cultured human primary macrophages and lymphocytes. J. Virol. 69:1995;7699-7711.
28. Schubert U., Ferrer-Montiel A. V., Oblatt-Montal M., Henklein P., Strebel K., Montal M. Identification of an ion channel activity of the Vpu transmembrane domain and its involvement in the regulation of virus release from HIV-1-infected cells. FEBS Lett. 398:1996b;12-18.
29. Schubert U., Strebel K. Differential activities of the human immunodeficiency virus type 1-encoded Vpu protein are regulated by phosphorylation and occur in different cellular compartments. J. Virol. 68:1994;2260-2271.
30. Schwartz M. D., Geraghty R. J., Panganiban A. T. HIV-1 particle release mediated by Vpu is distinct from that mediated by p6. Virology. 224:1996;302-309.
31. Spearman P., Horton R., Ratner L., Kuli-Zade I. Membrane binding of human immunodeficiency virus type 1 matrix protein in vivo supports a conformational myristyl switch mechanism. J. Virology. 71:1997;6582-6592.
32. Spearman P., Ratner L. Human immunodeficiency virus type 1 capsid formation in reticulocyte lysates. J. Virol. 70:1996;8187-8194.
33. Spearman P., Wang J. J., Vander Heyden N., Ratner L. Identification of human immunodeficiency virus type 1 Gag protein domains essential to membrane binding and particle assembly. J. Virol. 68:1994;3232-3242.
34. Strebel K., Klimkait T., Martin M. A. A novel gene of HIV-1, vpu, and its 16-kilodalton product. Science. 241:1988;1221-1223.
35. Verderame M. F., Nelle T. D., Wills J. W. The membrane-binding domain of the Rous sarcoma virus Gag protein. J. Virol. 70:1996;2664-2668.
36. Wang J., Horton R., Varthakavi V., Spearman P., Ratner L. Formation and release of virus-like particles by HIV-1 matrix protein [letter]. AIDS. 13:1999;281-283.
37. Yao X. J., Friborg J., Checroune F., Gratton S., Boisvert F., Sekaly R. P., Cohen E. A. Degradation of CD4 induced by human immunodeficiency virus type 1 Vpu protein: A predicted alpha-helix structure in the proximal cytoplasmic region of CD4 contributes to Vpu sensitivity. Virology. 209:1995;615-623.
38. Zhang Y., Qian H., Love Z., Barklis E. Analysis of the assembly function of the human immunodeficiency virus type 1 gag protein nucleocapsid domain. J. Virol. 72:1998;1782-1789.
39. Zhou W., Resh M. D. Differential membrane binding of the human immunodeficiency virus type 1 matrix protein. J. Virol. 70:1996;8540-8548.