Quantification of VP22-GFP Spread by Direct Fluorescence in 15 Commonly Used Cell Lines

Journal of Gene Medicine

Volumn 1, Issue 4, 1999, Pages 265-274

  Wybranietz, W A ^a   Prinz, F ^a   Spiegel, M ^a   Schenk, A ^a   Bitzer, M ^a   Gregor, M ^a   Lauer, U M ^a  

^a UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

Author keywords

Gene therapy; GFP; HSV 1; Intercellular trafficking; Protein delivery; VP22

Indexed keywords

HUMAN HERPESVIRUS 1; MAMMALIA;

GREEN FLUORESCENT PROTEIN; HERPES SIMPLEX VIRUS TYPE 1 PROTEIN VP22; HYBRID PROTEIN; PHOTOPROTEIN; VIRUS PROTEIN;

ACTIVE TRANSPORT; ANIMAL; ARTICLE; CAT; CELL LINE; COMPARATIVE STUDY; CONFOCAL MICROSCOPY; DOG; GENE THERAPY; GENETIC TRANSFECTION; GENETICS; HAMSTER; HUMAN; METABOLISM; MOUSE; RAT; SPECIES DIFFERENCE; TISSUE DISTRIBUTION;

ANIMALS; BIOLOGICAL TRANSPORT, ACTIVE; CATS; CELL LINE; CRICETINAE; DOGS; GENE THERAPY; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MICE; MICROSCOPY, CONFOCAL; RATS; RECOMBINANT FUSION PROTEINS; SPECIES SPECIFICITY; TISSUE DISTRIBUTION; TRANSFECTION; VIRAL STRUCTURAL PROTEINS;

EID: 0033154928     PISSN: 1099498X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1521-2254(199907/08)1:4<265::AID-JGM48>3.0.CO;2-D     Document Type: Article

References (9)

1. Elliott GD, Meredith DM. The herpes simplex virus type 1 tegument protein VP22 is encoded by gene UL49. J Gen Virol 1992; 73: 723-726.
2. Elliott GD, O'Hare P. Intercellular trafficking and protein delivery by a herpesvirus structural protein. Cell 1997; 88: 223-233.
3. Elliott GD, O'Hare P. Herpes simplex virus type 1 tegument protein VP22 induces the stabilization and hyperacetylation of microtubules. J Virol 1998; 72: 6448-6455.
4. Phelan A, Elliott GD, O'Hare P. Intercellular delivery of functional p53 by the herpesvirus protein VP22. Nat Biotechnol 1998; 16: 440-443.
5. Dilber MS, Phelan A, Aints A, Mohamed AJ, Elliott GD, Smith CIE, et al. Intercellular delivery of thymidine kinase prodrug activating enzyme by the herpes simplex virus protein VP22. Gene Ther 1999; 6: 12-21.
6. Fuhrer C, Geffen I, Huggel K, Spiess M. The two subunits of the asialoglycoprotein receptor contain different sorting information. J Biol Chem 1994; 269: 3277-3282.
7. Murphy AL, Murphy SJ. Catch VP22: the hitch-hiker's ride to gene therapy? Gene Ther 1999; 6: 4-5.
8. Fang B, Xu B, Koch P, Roth JA. Intercellular trafficking of VP22-GFP fusion proteins is not observed in cultured mammalian cells. Gene Ther 1998; 5: 1420-1424.
9. Elliott GD, O'Hare P. Intercellular trafficking of VP22-GFP fusion proteins. Gene Ther 1999; 6: 149-151.