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Volumn 13, Issue 3, 2001, Pages 159-167
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Identification of substrate specificity determinants in human cAMP-specific phosphodiesterase 4A by single-point mutagenesis
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Author keywords
Aspartic acid 440; cAMP specific phosphodiesterase 4A; Single point mutagenesis; Substrate specificity
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Indexed keywords
8 METHOXY 5 N PROPYL 3 METHYL 1 ETHYL IMIDAZO[1,5 A]PYRIDO[3,2 E]PYRAZINONE;
ALANINE;
AROFYLLINE;
ASPARAGINE;
ASPARTIC ACID;
CELL DNA;
CYCLIC AMP;
CYCLIC GMP;
CYSTEINE;
D 22888;
LEUCINE;
PHENYLALANINE;
PHOSPHODIESTERASE IV;
PHOSPHODIESTERASE IV INHIBITOR;
PICLAMILAST;
PROLINE;
ROLIPRAM;
TRYPTOPHAN;
TYROSINE;
UNCLASSIFIED DRUG;
VALINE;
ARTICLE;
BINDING AFFINITY;
CATALYSIS;
CELL STRAIN COS1;
DRUG SENSITIVITY;
ENZYME ACTIVE SITE;
ENZYME ACTIVITY;
ENZYME ASSAY;
ENZYME SPECIFICITY;
ENZYME STRUCTURE;
HUMAN;
HUMAN CELL;
HYDROLYSIS;
IC 50;
LEUKOCYTE;
NUCLEOTIDE SEQUENCE;
POINT MUTATION;
PRIORITY JOURNAL;
PROTEIN EXPRESSION;
SEQUENCE HOMOLOGY;
SITE DIRECTED MUTAGENESIS;
3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE;
AMINO ACID SEQUENCE;
AMINO ACID SUBSTITUTION;
ASPARTIC ACID;
BINDING, COMPETITIVE;
BLOTTING, WESTERN;
CELLS, CULTURED;
CYCLIC AMP;
CYCLIC GMP;
DNA, COMPLEMENTARY;
ELECTROPHORESIS, POLYACRYLAMIDE GEL;
GENE EXPRESSION REGULATION, ENZYMOLOGIC;
HUMANS;
ISOENZYMES;
MOLECULAR SEQUENCE DATA;
MUTAGENESIS, SITE-DIRECTED;
PHOSPHODIESTERASE INHIBITORS;
POLYMERASE CHAIN REACTION;
RNA, MESSENGER;
ROLIPRAM;
SEQUENCE ALIGNMENT;
SUBSTRATE SPECIFICITY;
OXY;
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EID: 0035082452
PISSN: 08986568
EISSN: None
Source Type: Journal
DOI: 10.1016/S0898-6568(01)00142-5 Document Type: Article |
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Times cited : (15)
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References (43)
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