Mapping the functional domains of human recombinant phosphodiesterase 4A: Structural requirements for catalytic activity and rolipram binding

Molecular Pharmacology

Volumn 50, Issue 4, 1996, Pages 891-899

  Jacobitz, Susanne ^a   Mclaughlin, Megan M ^a   Livi, George P ^a   Burman, Miriam ^a   Torphy, Theodore J ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHODIESTERASE; PICLAMILAST; ROLIPRAM;

ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CONFORMATIONAL TRANSITION; DRUG PROTEIN BINDING; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ENZYME INHIBITION; HUMAN; NONHUMAN; PEPTIDE MAPPING; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION;

3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE; BASE SEQUENCE; BENZAMIDES; BINDING SITES; BINDING, COMPETITIVE; CATALYSIS; KINETICS; MOLECULAR SEQUENCE DATA; PEPTIDE MAPPING; PHOSPHODIESTERASE INHIBITORS; PHOSPHORIC DIESTER HYDROLASES; PROTEIN STRUCTURE, TERTIARY; PYRIDINES; PYRROLIDINONES; RECOMBINANT PROTEINS; ROLIPRAM; STRUCTURE-ACTIVITY RELATIONSHIP; TRITIUM;

EID: 0029979738     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (33)

1. Beavo, J. A., and D. H. Reifsnyder. Primary sequence of cyclic nucleotide phosphodiesterase isozymes and the design of selective inhibitors. Trends Pharmacol. Sci. 11:150-155 (1990).
2. Bentley, J. K., and J. A. Beavo. Regulation and function of cyclic nucleotides. Curr. Opin. Cell Biol. 4:233-240 (1992).
3. Beavo, J. A., M. Conti, and R. J. Heaslip. Multiple cyclic nucleotide phosphodiesterases. Mol. Pharmacol. 46:399-405 (1994).
4. Ashton, M. J., D. C. Cook, G. Fenton, J.-A, Karlsson, M. N. Palfreyman, D. Raeburn, A. J. Ratcliffe, J. E. Souness, S. Thurairatnam, and N. Vicker. Selective type IV phosphodiesterase inhibitors as antiasthmatic agents: the syntheses and biological activities of 3-(cyclopentyloxy)-4-methoxybenzamides and analogues. J. Med. Chem. 37:1696-1703 (1994)
5. Torphy, T. J., and B. J. Undem. Phosphodiesterase inhibitors: new opportunities for the treatment of asthma. Thorax 46:512-524 (1991).
6. Swinnen, J. V., D. R. Josef, and M. Conti. Molecular cloning of rat homologues of the Drosophila melanogaster dunce cAMP phosphodiesterase: evidence for a family of genes. Proc. Natl. Acad. Sci. USA 86:5325-5329 (1989).
7. Colicelli, J., C. Birchmeier, J. Michaeli, K. O'Neill, M. Riggs, and M. Wigler. Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase. Proc. Natl. Acad. Sci. USA 86: 3599-3603 (1989).
8. m, rolipram sensitive cyclic AMP phosphodiesterase. Mol. Cell. Biol. 10:2678-2686 (1990).
9. m, rolipram-sensitive cAMP phosphodiesterase. J. Biol. Chem. 267:18929-18939 (1992).
10. m, rolipram-sensitive, cAMP-specific phosphodiesterase from human brain. J. Biol. Chem. 268:6470-6476 (1993).
11. Obernolte, R., S. Bhakta, R. Alvarez, C. Bach, P. Zuppan, M. Mulkins, K. Jarnagin, and E. R. Shelton. The cDNA of a human lymphocyte cyclic-AMP phosphodiesterase (PDE IV) reveals a multigene family. Gene 129:239-247 (1993).
12. Bolger, G.,T. Michaeli, T. Martins, T. St. John, B. Steiner, L. Rodgers, M. Riggs, M. Wigler, and K. Ferguson. A family of human phosphodiesterases homologous to the dunce learning and memory gene product of Drosophila melanogaster are potential targets for antidepressant drugs. Mol. Cell. Biol. 13:6558-6571 (1993).
13. Bolger, G. B., L. Rodgers, and M. Riggs. Differential CNS expression of alternative mRNA isoforms of the mammalian genes encoding cAMP-specific phosphodiesterases. Gene 149:237-244 (1994).
14. Monaco, L., E. Vicini, and M. Conti. Structure of two rat genes coding for closely related rolipram-sensitive cAMP phosphodiesterases. J. Biol. Chem. 269:347-357 (1994).
15. D). Gene 138:253-256 (1994).
16. Schneider, H. H., R. Schmiechen, M. Brezenski, and J. Seidler. Stereospecific binding of the antidepressant rolipram to brain protein structures. Eur. J. Pharmacol. 127:105-115 (1986).
17. Torphy, T. J., J. M. Stadel, M. Burman, L. B. Cieslinski, M. M. McLaughlin, J. R. White, and G. P. Livi. Coexpression of human cAMP-specific phosphodiesterase activity and high affinity rolipram binding in yeast. J. Biol. Chem. 267:1798-1804 (1992).
18. Souness, J. E., C. Maslen, and L. C. Scott. Effects of solubilization and vanadate/glutathion complex on inhibitor potencies against eosinophil cyclic AMP-specific phosphodiesterase. FEBS Lett. 302:181-184 (1992).
19. McHale, M. M., L. B. Cieslinski, W.-K. Eng, R. K. Johnson, T. J. Torphy, and G. P. Livi. Expression of human recombinant cAMP phosphodiesterase isozyme IV reverses growth arrest phenotypes in phosphodiesterase-deficient yeast. Mol. Pharmacol. 39:109-113 (1991).
20. h6.1). Cell. Signalling 6:793-812 (1994).
21. Torphy, T. J., W. E. DeWolf, Jr., D. W. Green, and G. P. Livi. Biochemical characteristics and cellular regulation of phosphodiesterase IV. Agents Actions 43:51-71 (1993).
22. Mullis, K. B., and F. A. Faloona. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 155:335-350 (1987).
23. Ito, H., Y. Fukuda, K. Murata, and A. Kimura. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163-168 (1983).
24. Nikawa, J.-I., P. Sass, and M. Wigler. Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae. Mol Cell. Biol. 7:3629-3636 (1987).
25. Sass, P., J. Field, J.-I. Nikawa, T. Toda, and M. Wigler. Cloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 83:9303-9307 (1986).
26. m cyclic AMP phosphodiesterase of Saccharomyces cerevisiae. Mol. Cell. Biol. 8:505-510 (1988).
27. Torphy, T. J., and L. B. Cieslinski. Characterization and selective inhibition of cyclic nucleotide phosphodiesterase isozymes in canine tracheal smooth muscle. Mol. Pharmacol. 37:206-214 (1990).
28. Engels, P., M. Sullivan, T. Müller, and H. Lübbert. Molecular cloning and functional expression in yeast of a human cAMP-specific phosphodiesterase subtype (PDE IV-C). FEBS Lett. 358:305-310 (1995).
29. + gene. Proc. Natl. Acad. Sci. USA 86:3604-3608 (1989).
30. + gene, structural gene for cAMP phosphodiesterase. Proc. Natl. Acad. Sci. USA 83:9313-9317 (1986).
31. Torphy T. J., and G. P. Livi. Phosphodiesterase isozymes in airways, in Pharmacology of the Respiratory Tract (K. F. Chung and (P. J. Barnes, eds.). Marcel Dekker, New York, 177-222 (1993).
32. Pillai, R., K. Kytle, A. Reyes, and J. Colicelli. Use of a yeast expression system for the isolation and analysis of drug-resistant mutants of a mammalian phosphodiesterase. Proc. Natl. Acad. Sci. USA 90:11970-11974 (1993).
33. Conti, M., G. Nemoz, C. Sette, and E. Vicini. Recent progress in understanding the hormonal regulation of phosphodiesterases. Endocr. Rev. 16:370-389 (1995).