Analysis of a mutation in phosphodiesterase type 4 that alters both inhibitor activity and nucleotide selectivity

Molecular Pharmacology

Volumn 57, Issue 5, 2000, Pages 991-999

  Herman, Sarah B ^a   Juilfs, Dawn M ^a   Fauman, Eric B ^a   Juneau, Paul ^a   Menetski, Joseph P ^a  

^a PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

1 (3 CHLOROANILINO) 4 PHENYLPHTHALAZINE; 3 ISOBUTYL 8 METHOXYMETHYL 1 METHYLXANTHINE; 4 (3 BUTOXY 4 METHOXYBENZYL) 2 IMIDAZOLIDINONE; CILOSTAMIDE; CYCLIC AMP; CYCLIC GMP; DIPYRIDAMOLE; ETAZOLATE; ISOBUTYLMETHYLXANTHINE; MILRINONE; MUTANT PROTEIN; PHOSPHODIESTERASE; PHOSPHODIESTERASE INHIBITOR; QUAZINONE; ROLIPRAM; TREQUINSIN; VINPOCETINE; ZAPRINAST;

AMINO ACID SEQUENCE; ARTICLE; CELL LYSATE; ENZYME ACTIVITY; ENZYME INHIBITION; HYDROLYSIS; MUTAGENESIS; NONHUMAN; PLASMID; PRIORITY JOURNAL;

3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE; AMINO ACID SEQUENCE; ANIMALS; BINDING, COMPETITIVE; CELLS, CULTURED; HUMANS; INSECTS; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; NUCLEOTIDES; PHOSPHODIESTERASE INHIBITORS; PURINONES; RATS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

EID: 0034060376     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (40)

1. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W and Lipman DJ (1997) Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res 25:3389-3402.
2. Atienza JM and Colicelli J (1998) Yeast model system for study of mammalian phosphodiesterases. Methods 14:35-42.
3. Atienza JM, Susanto D, Huang C, McCarty AS and Colicelli J (1999) Identification of inhibitor specificity determinants in a mammalian phosphodiesterase. J Biol Chem 274:4839-4847.
4. Beavo JA (1995) Cyclic nucleotide phosphodiesterases: Functional implications of multiple isoforms. Physiol Rev 75:725-748.
5. Beltman J, Becker DE, Butt E, Jensen GS, Rybalkin SD, Jastorff B and Beavo JA (1995) Characterization of cyclic nucleotide phosphodiesterases with cyclic GMP analogs: Topology of the catalytic domains. Mol Pharmacol 47:330-339.
6. Billingsley P (1986) Probability and Measure. Wiley, New York.
7. Bolger G, Michaeli T, Martins T, St. John T, Steiner B, Rodgers L, Riggs M, Wigler M and Ferguson K (1993) A family of human phosphodiesterases homologous to the dunce learning and memory gene product of Drosophila melanogaster are potential targets for antidepressant drugs. Mol Cell Biol 13:6558-6571.
8. Butt E, Beltman J, Becker DE, Jensen GS, Rybalkin SD, Jastorff B and Beavo JA (1995) Characterization of cyclic nucleotide phosphodiesterases with cyclic AMP analogs: Topology of the catalytic sites and comparison with other cyclic AMP-binding proteins. Mol Pharmacol 47:340-347.
9. Charbonneau H, Beier N, Walsh KA and Beavo JA (1986) Identification of a conserved domain among cyclic nucleotide phosphodiesterases from diverse species. Proc Natl Acad Sci USA 83:9308-9312.
10. Conti M and Jin SL (1999) The molecular biology of cyclic nucleotide phosphodiesterases. Prog Nucl Acid Res Mol Biol 63:1-38
11. Degerman E, Beifrage P and Manganiello VC (1997) Structure, localization, and regulation of cGMP-inhibited phosphodiesterase (PDE3). J Biol Chem 272:6823-6826.
12. Fisher DA, Smith JF, Pillar JS, St Denis SH and Cheng JB (1998a) Isolation and characterization of PDE8A, a novel human cAMP-specific phosphodiesterase. Biochem Biophys Res Commun 248:570-577.
13. Fisher DA, Smith JF, Pillar JS, St Denis SH and Cheng JB (1998b) Isolation and characterization of PDE9A, a novel human cGMP-specific phosphodiesterase. J Biol Chem 273:15559-15564.
14. Fujishige K, Kotera J, Michibata H, Yuasa K, Takebayashi S, Okumura K and Omori K (1999) Cloning and characterization of a novel human phosphodiesterase that hydrolyzes both cAMP and cGMP (PDE10A). J Biol Chem 274:18438-18445.
15. Hardy LW and Nalivaika E (1992) Asn177 in Escherichia coli thymidylate synthase is a major determinant of pyrimidine specificity. Proc Natl Acad Sci USA 89:9725-9729.
16. Henikoff S and Henikoff JG (1993) Performance evaluation of amino acid substitution matrices. Proteins 17:49-61.
17. Houslay MD, Sullivan M and Bolger GB (1998) The multienzyme PDE4 cyclic adenosine monophosphate-specific phosphodiesterase family: Intracellular targeting, regulation, and selective inhibition by compounds exerting anti-inflammatory and antidepressant actions. Adv Pharmacol 44:225-342
18. Iona S, Cuomo M, Bushnik T, Bushnik N, Sette C, Hess M, Shelton ER and Conti M (1998) Characterization of the rolipram-sensitive, cyclic AMP-specific phosphodiesterases: Identification and differential expression of immunologically distinct forms in the rat brain. Mol Pharmacol 53:23-32.
19. Jacobitz S, McLaughlin MM, Livi GP, Burman M and Torphy TJ (1996) Mapping the functional domains of human recombinant phosphodiesterase 4A: Structural requirements for catalytic activity and rolipram binding. Mol Pharmacol 50:891-899.
20. Liu L and Santi DV (1992) Mutation of asparagine 229 to aspartate in thymidylate synthase converts the enzyme to a deoxycytidylate methylase. Biochemistry 31: 5100-5104.
21. Liu L and Santi DV (1993) Exclusion of 2′-deoxycytidine 5′-monophosphate by asparagine 229 of thymidylate synthase. Biochemistry 32:9263-9267.
22. McPhee I, Yarwood S, Scotland G, Scotland H, Beard MB, Ross AH, Houslay ES and Houslay MD (1999) Association with the SRC family tyrosyl kinase LYN triggers a conformational change in the catalytic region of human cAMP-specific phosphodiesterase HSPDE4A4B. J Biol Chem 274:11796-11810.
23. Nemoz G, Zhang R, Sette C and Conti M (1996) Identification of cyclic AMP-phosphodiesterase variants from the PDE4D gene expressed in human peripheral mononuclear cells. FEBS Lett 384:97-102.
24. Omburo GA, Torphy TJ, Scott G, Jacobitz S, Colman RF and Colman RW (1997) Inactivation of recombinant monocyte cAMP-specific phosphodiesterase by cAMP analog, 8-[(4-bromo-2,3-dioxobutyl)thio]adenosine 3′,5′-cyclic monophosphate. Blood 89:1019-1026.
25. Pillai R, Kytle K, Reyes A and Colicelli J (1993) Use of a yeast expression system for the isolation and analysis of drug-resistant mutants of a mammalian phosphodiesterase. Proc Natl Acad Sci USA 90:11970-11974.
26. Rocque WJ, Holmes WD, Patel IR, Dougherty RW, Itto-op O, Overton L, Hoffman CR, Wisely GB, Willard DH and Luther MA (1997) Detailed characterization of a purified type 4 phosphodiesterase, HSPDE4B2B: Differentiation of high- and low-affinity (R)-rolipram binding. Prot Exp Purif 9:191-202.
27. Salanova M, Jin SC and Conti M (1998) Heterologous expression and purification of recombinant rolipram-sensitive cyclic AMP-specific phosphodiesterases. Methods 14:55-64.
28. Saldou N, Baecker PA, Li B, Yuan Z, Obernolte R, Ratzliff J, Osen E, Jarnagin K and Shelton ER (1998a) Purification and physical characterization of cloned human cAMP phosphodiesterases PDE-4D and -4C. Cell Biochem Biophys 28:187-217.
29. Saldou N, Obernolte R, Huber A, Baecker PA, Wilhelm R, Alvarez R, Li B, Xia L, Callan O, Su C, Jarnagin K and Shelton ER (1998b) Comparison of recombinant human PDE4 isoforms: Interaction with substrate and inhibitors. Cell Signal 10:427-440.
30. Soderling SH, Bayuga SJ and Beavo JA (1998a) Cloning and characterization of a cAMP-specific cyclic nucleotide phosphodiesterase. Proc Natl Acad Sci USA 95: 8991-8996.
31. Soderling SH, Bayuga SJ and Beavo JA (1998b) Identification and characterization of a novel family of cyclic nucleotide phosphodiesterases. J Biol Chem 273:15553-15558.
32. Soderling SH, Bayuga SJ and Beavo JA (1999) Isolation and characterization of a dual-substrate phosphodiesterase gene family: PDE10A. Proc Natl Acad Sci USA 96:7071-7076.
33. Spina D, Landells LJ and Page CP (1998) The role of phosphodiesterase enzymes in allergy and asthma. Adv Pharmacol 44:33-89
34. Teixeira MM, Gristwood RW, Cooper N and Hellewell PG (1997) Phosphodiesterase (PDE)4 inhibitors: Anti-inflammatory drugs of the future? Trends Pharmacol Sci 18:164-171.
35. Torphy TJ (1998) Phosphodiesterase isozymes: Molecular targets for novel anti-asthma agents. Am J Respir Crit Care Med 157:351-370.
36. Turko IV, Francis SH and Corbin JD (1998a) Hydropathic analysis and mutagenesis of the catalytic domain of the cGMP-binding cGMP-specific phosphodiesterase (PDE5). cGMP versus cAMP substrate selectivity. Biochemistry 37:4200-4205.
37. Turko IV, Francis SH and Corbin JD (1998b) Potential roles of conserved amino acids in the catalytic domain of the cGMP-binding cGMP-specific phosphodiesterase. JBiol Chem 273:6460-6466.
38. Wilson M, Sullivan M, Brown N and Houslay MD (1994) Purification, characterization and analysis of rolipram inhibition of a human type-IVA cyclic AMP-specific phosphodiesterase expressed in yeast. Biochem J 304:407-415.
39. Yarwood SJ, Steele MR, Scotland G, Houslay MD and Bolger GB (1999) The RACK1 signaling scaffold protein selectively interacts with the cAMP-specific phosphodiesterase PDE4D5 isoform. J Biol Chem 274:14909-14917.
40. Zhou XH, Gao S and Hui SL (1997) Methods for comparing the means of two independent log-normal samples [published erratum appears in Biometrics (1997) 53:1566]. Biometrics 53:1129-1135.