Atomic structure of PDE4: Insights into phosphodiesterase mechanism and specificity

Science

Volumn 288, Issue 5472, 2000, Pages 1822-1825

  Xu, Robert X ^a   Hassell, Anne M ^a   Vanderwall, Dana ^a   Lambert, Millard H ^a   Holmes, William D ^a   Luther, Michael A ^a   Rocque, Warren J ^a   Milburn, Michael V ^a   Zhao, Yingdong ^b,c   Ke, Hengming ^b   Nolte, Robert T ^a  

^a GLAXOSMITHKLINE   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^c NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIC NUCLEOTIDE; PHOSPHODIESTERASE IV;

ARTICLE; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME STRUCTURE; MOLECULAR WEIGHT; PRIORITY JOURNAL;

3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE; BINDING SITES; CATALYTIC DOMAIN; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; CYCLIC AMP; CYCLIC GMP; HYDROGEN BONDING; HYDROLYSIS; METALS; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; SUBSTRATE SPECIFICITY;

EID: 0034625541     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.288.5472.1822     Document Type: Article

References (37)

1. M. Conti and S. L. C. Jin, Prog. Nucleic Acid Res. Mol. Biol. 63, 1 (1999).
2. M. D. Houslay, M. Sullivan, G. B. Bolger, Adv. Pharmacol. 44, 225 (1998).
3. V. C. Manganiello, T. Murata, M. Taira, P. Belfrage, E. Degerman, Arch. Biochem. Biophys. 322, 1 (1995).
4. T. J. Torphy, Asthma 2, 1755 (1997).
5. A. Z. Zhao, C. Yan, W. K. Sonnenburg, J. A. Beavo, Adv. Second Messenger Phosphoprotein Res. 31, 237 (1997).
6. K. Loughney et al., Gene 234, 109 (1999).
7. M. D. Houslay and G. Milligan, Trends Biochem. Sci. 22, 217 (1997).
8. J. A. Beavo, Physiol. Rev. 75, 725 (1995).
9. R. E. Weishaar, M. H. Cain, J. A. Bristol, J. Med. Chem. 28, 537 (1985).
10. M. Conti, S. L. C. Jin, L. Monaco, D. R. Repaske, J. V. Swinnen, Endocr. Rev. 12, 218 (1991).
11. M. M. Teixeira, R. W. Gristwood, N. Cooper, P. G. Hellewell, Trends Pharmacol. Sci. 18, 164 (1997).
12. 2-terminal residue 152 to residue 489 for both molecules in the asymmetric unit. No density was observed for residues 490 to 495 and residues 509 to 528.
13. For sequence alignment and secondary structure assignment of representative members from the 10 PDE gene families, see Science Online (www. sciencemag.org/feature/data/1050158.shl).
14. M. D. Houslay, Semin. Cell Dev. Biol. 9, 161 (1998).
15. J. Londesborough, Biochem. J. 225, 143 (1985).
16. R. J. Marchmont and M. D. Houslay, Nature 286, 904 (1980).
17. R. J. Marchmont, S. R. Ayad, M. D. Houslay, Biochem. J. 195, 645 (1981).
18. S. H. Francis, J. L. Colbran, L. M. McAllister-Lucas, J. D. Corbin, J. Biol. Chem. 269, 22477 (1994).
19. M. D. Percival, B. Yeh, J.-P. Falgueyret, Biochem. Biophys. Res. Commun. 241, 175 (1997).
20. S. M. Callahan, W. Cornell Neal, P. V. Dunlap, J. Biol. Chem. 270, 17627 (1995).
21. I. Wilkinson, P. Engels, M. D. Houslay, Pharmacol. Rev. Commun. 9, 215 (1997).
22. G. A. Omburo, S. Jacobitz, T. J. Torphy, R. W. Colman, Cell. Signalling 10, 491 (1998).
23. L. S. Beese and T. A. Steitz, EMBO J. 10, 25 (1991).
24. S. L. C. Jin, J. V. Swinnen, M. Conti, J. Biol. Chem. 267, 18929 (1992).
25. I. V. Turko, S. H. Francis, J. D. Corbin, J. Biol. Chem. 273, 6460 (1998).
26. G. P. Livi et al., Mol. Cell. Biol. 10, 2678 (1990).
27. W. J. Rocque et al., Biochemistry 36, 14250 (1997).
28. J. M. Atienza, D. Susanto, C. Huang, A. S. McCarty, J. Colicelli, J. Biol. Chem. 274, 4839 (1999).
29. W. J. Rocque et al., Protein Expr. Purif. 9, 191 (1997).
30. Collaborative Computational Project No. 4, Acta Crystallogr. D50, 760 (1994).
31. E. De La Fortelle and G. Bricogne, Methods Enzymol. 276, 472 (1997).
32. T. A. Jones, J. Y. Zou, S. W. Cowan, M. Kjeldgaard, Acta Crystallogr. A47, 110 (1991).
33. A. T. Brünger et al., Acta Crystallogr. D54, 905 (1998).
34. 2+, respectively, and the low-occupancy arsenate ion was omitted.
35. E. Butt et al., Mol. Pharmacol. 47, 340 (1995).
36. Z. Otwinowski and W. Minor, Methods Enzymol. 276, 307 (1997).
37. Coordinates of the PDE4B2B catalytic domain may be obtained from the Protein Data Bank, accession code 1FOJ. We thank R. Sweet (NSLS beamline X12C), J. Chrzas (APS beamline 17-ID), Y. Xia and R. Gampe for technical assistance, E. Xu for useful discussions, and P. Jeffs for encouragement and support. Supported by NIH grant AI33072 (H.K.).