The ATP requirement for U2 snRNP addition is linked to the pre-mRNA region 5′ to the branch site

RNA

Volumn 7, Issue 9, 2001, Pages 1298-1309

  Newnham, Catherine M ^a   Query, Charles C ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

Anchoring site; ATP; Intron; Pre mRNA; Spliceosome; U2 snRNP

Indexed keywords

ADENOSINE TRIPHOSPHATE; MESSENGER RNA PRECURSOR; RIBONUCLEASE H; SMALL NUCLEAR RIBONUCLEOPROTEIN;

ARTICLE; COMPLEX FORMATION; INTRON; MOLECULAR INTERACTION; PRIORITY JOURNAL; RNA CLEAVAGE; RNA SEQUENCE; SPLICEOSOME;

5' UNTRANSLATED REGIONS; ADENOSINE TRIPHOSPHATE; RIBONUCLEOPROTEIN, U2 SMALL NUCLEAR; RNA PRECURSORS; RNA SPLICE SITES; RNA SPLICING;

EID: 0034840125     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838201010561     Document Type: Article

References (55)

1. Lethal and temperature-sensitive mutations and their suppressors identify an essential structural element in U2 small nuclear RNA
2. Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction
3. The splicing factor BBP interacts specifically with the pre-mRNA branch-point sequence UACUAAC
4. U2 as well as U1 small nuclear ribonucleo proteins are involved in premessenger RNA splicing
5. Splicing of precursors to mRNAs by the spliceosomes
6. Specific alterations of U1-C protein or U1 small nuclear RNA can eliminate the requirement of Prp28p, an essential DEAD box splicing factor
7. Spliceosome assembly in yeast
8. PRP5: A helicase-like protein required for mRNA splicing in yeast
9. Functional association of U2 snRNP with the ATP-independent spliceosomal complex E
10. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
11. U2AF65 recruits a novel human DEAD box protein required for the U2 snRNPbranchpoint interaction
12. Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A
13. A potential role for U2AF-SAP 155 interactions in recruiting U2 snRNP to the branch site
14. Messenger RNA splicing in vitro: An excised intervening sequence and a potential intermediate
15. The 100-kda U5 snRNP protein (hPrp28p) contacts the 5′ splice site through its ATPase site
16. Inhibition by SR proteins of splicing of a regulated adenovirus pre-mRNA
17. Regulation of adenovirus alternative RNA splicing by dephosphorylation of SR proteins
18. Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for Sub2, an essential spliceosomal ATPase
19. Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs
20. Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes
21. Presplicing complex formation requires two proteins and U2 snRNR
22. The structure and function of proteins involved in mammalian pre-mRNA splicing
23. Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP
24. Requirements for U2 snRNP addition to yeast pre-mRNA
25. Multiple roles for the yeast SUB2/yUAP56 gene in splicing
26. An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway
27. Joining of RNAs by splinted ligation
28. Splicing of precursors to mRNA by the spliceosome
29. RNA-RNA interactions in nuclear pre-mRNA splicing
30. The Saccharomyces cerevisiae Prp5 protein has RNA-dependent ATPase activity with specificity for U2 small nuclear RNA
31. Recognition of the TAC TAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA
32. Functional Cus1p is found with Hsh155p in a multiprotein splicing factor associated with U2 snRNA
33. ATP can be dispensable for prespliceosome formation in yeast
34. Assemblage of the prespliceosome complex with separated fractions isolated from HeLa cells
35. A minimal spliceosomal A complex recognizes branch site and polypyrimidine tract
36. RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2
37. A spliceosomal recycling factor that reanneals U4 and U6 small nuclear ribonucleoprotein particles
38. Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA
39. A new twist on RNA helicases: DExH/D box proteins as RNPases
40. Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing
41. A conformational rearrangement in the spliceosome is dependent on PRP16 and ATP hydrolysis
42. RNA helicase dynamics in pre-mRNA splicing
43. Mechanical devices of the spliceosome: Motors, clocks, springs, and things
44. An RNA switch at the 5′ splice site requires ATP and the DEAD box protein Prp28p
45. Adenosine phosphorothioates (ATPαS and ATPτS) differentially affect the two steps of mammalian pre-mRNA splicing
46. Interactions between highly conserved U2 small nuclear RNA structures and Prp5p, Prp9p, Prp11p, and Prp21p proteins are required to ensure integrity of the U2 small nuclear ribonucleoprotein in Saccharomyces cerevisiae
47. In vitro studies of the Prp9.Prp11.Prp21 complex indicate a pathway for U2 small nuclear ribonucleoprotein activation
48. snRNP structure and function
49. RNP remodeling with DExH/D boxes
50. A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site
51. Synthesis, deprotection, analysis and purification of RNA and ribozymes
52. Mammalian pre-mRNA branch site selection by U2 snRNP involves base pairing
53. Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits
54. Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56
55. A compensatory base change in human U2 snRNA can suppress a branch site mutation