Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56

Genes and Development

Volumn 15, Issue 1, 2001, Pages 30-35

  Zhang, M ^a   Green, M R ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

Commitment complex; DEAD box protein; HUAp56; Pre mRNA splicing; SUB2; U2 snRNP binding; U2AF

Indexed keywords

HOMEODOMAIN PROTEIN; MESSENGER RNA PRECURSOR; SMALL NUCLEAR RIBONUCLEOPROTEIN;

ARTICLE; HOMEOBOX; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; RNA SPLICING; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SPLICEOSOME;

ADENOSINE TRIPHOSPHATASES; AMINO ACID SEQUENCE; CLONING, MOLECULAR; CONSERVED SEQUENCE; FUNGAL PROTEINS; HUMANS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEAR PROTEINS; RECOMBINANT PROTEINS; RIBONUCLEOPROTEINS; RNA SPLICING; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

SACCHAROMYCES CEREVISIAE;

EID: 0035187881     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.851701     Document Type: Article

References (33)

1. Splicing of precursors to mRNAs by the spliceosome
2. PRP5: A helicase-like prorein required for mRNA splicing in yeast
3. 65 recruits a novel human DEAD box protein required for the U2 sn-RNP-branchpoint interaction
4. A general method of in vitro preparation and specific mutagenesis of DNA fragments: Study of protein and DNA interactions
5. The spliceosome assembly pathway in mammalian extracts
6. Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for Sub2, an essential spliceosomal ATPase
7. The structure and function of proteins involved in mammalian pre-mRNA splicing
8. The human U5-200 kD DEXH-box protein unwinds U4/U6 RNA duplices in vitro
9. A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction
10. Requirements for U2 snRNP addition to yeast pre-mRNA
11. Multiple roles for the yeast SUB2/yUAP56 gene in splicing
12. An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway
13. Transcriptional pulse-chase analysis reveals a role for a novel snRNP-associated protein in the manufacture of spliceosomal snRNPs
14. The Saccharomyces cerevisiae Prp5 protein has RNA-dependent ATPase activity with specificity for U2 small nuclear RNA
15. Mutational analysis of a DEAD box RNA helicase: The mammalian translation initiation factor eIF-4A
16. The HRIGRXXR region of the DEAD box RNA helicase eukaryotic translation initiation factor 4A is required for RNA binding and ATP hydrolysis
17. A dominant negative mutation in the conserved RNA helicase motif 'SAT' causes splicing factor PRP2 to stall in spliceosomes
18. RNA unwinding in U4/U6 sn-RNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2
19. Four yeast spliceosomal proteins (PRP5 PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA
20. Translation initiation factor 4A from Saccharomyces cerevisiae: Analysis of residues conserved in the D-E-A-D family of RNA helicases
21. Identification of functional U1 sn-RNA-pre-mRNA complexes committed to spliceosome assembly and splicing
22. The yeast branchpoint sequence is not required for the formation of a stable U1 snRNA-pre-mRNA complex and is recognized in the absence of U2 snRNA
23. Mechanical devices of the spliceosome: Motors, clocks, springs, and things
24. Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions
25. The DEAH-box protein PRP22 is an ATPase that mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
26. Yeast TAF(II)145 required for transcription of G1/S cyclin genes and regulated by the cellular growth stare
27. Developmental regulation of SPO13, a gene required for separation of homologous chromosomes at meiosis I
28. The DEAH-box splicing factor Prp16 unwinds RNA duplexes in vitro
29. Interaction between highly conserved U2 small nuclear RNA structures and Prp5p, Prp9p, Prp11p, and Prp21p proteins are required to ensure integrity of the U2 small nuclear ribonucleoprotein in Saccharomyces cerevisiae
30. CUS1, a supressor of cold-sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP 145
31. In vitro studies of the Prp9.Prp11.Prp21 complex indicate a pathway for U2 small nuclear ribonucleoprotein activation
32. Protein functions in pre-mRNA splicing