Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits

Molecular and Cellular Biology

Volumn 16, Issue 3, 1996, Pages 818-828

  Yan, Dong ^a   Ares Jr , Manuel ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SMALL NUCLEAR RNA;

ARTICLE; CONTROLLED STUDY; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN RNA BINDING; RNA SEQUENCE; RNA SPLICING; SACCHAROMYCES CEREVISIAE;

MAMMALIA; SACCHAROMYCES CEREVISIAE;

EID: 0030039512     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.16.3.818     Document Type: Article

References (72)

1. Abovich, N., P. Legrain, and M. Rosbash. 1990. The yeast PRP6 gene encodes a U4/U6 small nuclear ribonucleoprotein particle (snRNP) protein. and the PRP9 gene encodes a protein required for U2 snRNP binding. Mol. Cell. Biol. 10:6417-6425.
2. Abovich, N., X. C. Liao, and M. Rosbash. 1994 The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition. Genes Dev 8:843-854.
3. Arenas, J. E., and J. N. Abelson. 1993 The Saccharomyces cerevisiae PRP21 gene product is an integral component of the prespliceosome. Proc Natl. Acad. Sci. USA 90:6771-6775.
4. Ares, M., Jr. Unpublished data.
5. Ares, M., Jr., and A. H. Igel. 1989 Phylogenetic comparison of U2 small nuclear RNA sequences sugaests a pseudoknotted structure. UCLA Symp. Mol Cell. Biol. 94:13-23.
6. Ares, M., Jr., and A. H. Igel. 1990. Lethal and temperature-sensitive mutations and their suppressors identify an essential structure element in U2 small nuclear RNA. Genes Dev. 4:2132-2145.
7. Ares, M., Jr., and B. Weiser. 1993. Rearrangement of snRNA structure during assembly and function of the spliceosome. Prog. Nucleic Acid Res. Mol Biol. 50:131-159.
8. Behrens, S.-E., F. Galisson, P. Legrain, and R. Luhrmann. 1993. Evidence that the 60-kDa protein of 17S U2 small nuclear ribonucleoprotein is immunologically and functionally related to the yeast PRP9 splicing factor and is required for the efficient formation of prespliceosomes. Proc. Natl. Acad. Sci. USA 90:8229-8233.
9. Behrens, S.-E., K. Tyc, B. Kastner, J. Reichelt, and R. Luhrmann. 1993. Small nuclear ribonucleoprotein (RNP) U2 contains numerous additional proteins and has a bipartite RNP structure under splicing conditions. Mol. Cell. Biol 13:307-319.
10. Bennett, M., and R. Reed. 1993. Correspondence between a mammalian spliceosome component and an essential yeast splicing factor. Science 262: 105-108.
11. Boeke, J. D., J. Trueheart, G. Natsoulis, and G. R. Fink. 1987. 5-Fluoroorotic acid as a selective agent in yeast molecular genetics Methods Enzymol. 154: 164-175.
12. Brosi, R., K. Groning, S.-E. Behrens, R. Luhrmann, and A. Kramer. 1993. Interaction of mammalian splicing factor SF3a with U2 snRNP and relation of its 50-kD subunit to yeast PRP9. Science 262:102-105.
13. Brosi, R., H.-P. Hauri, and A. Kramer. 1993. Separation of splicing factor SF3 into two components and purification of SF3a activity. J. Biol. Chem. 268:17640-17646.
14. Brow, D. A., and R. M. Vidaver. 1995. An element in human U6 RNA destabilizes the U4/U6 spliceosomal RNA complex. RNA 1:122-131.
15. Cech, T. R. 1985. RNA splicing: three themes with variations. Cell 43: 713-716.
16. Chang, T.-H., M. W. Clark, A. J. Lustig, M. E. Cusick, and J. Abelson. 1988. RNA 11 protein is associated with the yeast spliceosome and is localized in the periphery of the cell nucleus. Mol. Cell. Biol. 8:2379-2393.
17. Chapon, C., and P. Legrain. 1992. A novel gene, spp91-1, suppresses the splicing defect and the pre-mRNA nuclear export in the prp9-1 mutant. EMBO J. 11:3279-3288
18. Chattoo, B., F. Sherman, T. Fejellstedt, D. Menhnert, and M. Ogur. 1979. Selection of lys2 mutants of the yeast Saccharomyces cerevisiae by the utilization of alpha-aminoadipate. Genetics 93:51-65.
19. Datta, B., and A. M. Weiner. 1991. Genetic evidence for base pairing between U2 and U6 snRNA in mammalian mRNA splicing. Nature (London) 352:821-824.
20. Datta, B., and A. M. Weiner. 1993 The phylogenetically invariant ACAG AGA and AGC sequence of U6 small nuclear RNA are more tolerant of mutation in human cells than in Saccharomyces cerevisiae. Mol. Cell. Biol. 13: 5377-5382.
21. Dower, W. J., J. F. Miller, and C. W. Ragsdale. 1988. High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 16: 6127-6145
22. Fabrizio, P., and J. Abelson. 1990. Two domains of yeast U6 small nuclear RNA required for both steps of nuclear precursor messenger RNA splicing. Science 250:404-409
23. Fabrizio, P., D. S. McPheeters, and J. Abelson. 1989. In vitro assembly of yeast U6 snRNP: a functional assay. Genes Dev. 3:2137-2150.
24. Fortner, D. M., R. G. Troy, and D. A. Brow. 1994. A stem/loop in U6 RNA defines a conformational switch required for pre-mRNA splicing Genes Dev. 8:221-233.
25. Frank, D., B. Patterson, and C. Guthrie. 1992. Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing. Mol. Cell. Biol. 12:5197-5205.
26. Ghetti, A., M. Company, and J. Abelson. 1995. Specificity of Prp24 binding to RNA: a role for Prp24 in the dynamic interaction of U4 and U6 snRNAs. RNA 1:132-145.
27. Gozani, O., J. G. Patton, and R. Reed. 1994. A novel set of spliceosome-associated proteins (SAPs) and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction. EMBO J. 13: 3356-3367.
28. Guthrie, C., and B. Patterson. 1988. Spliceosomal snRNAs. Annu. Rev. Genet. 22:387-419.
29. Hausner, T. P., L. M. Giglio, and A. M. Weiner. 1990. Evidence for base-pairing between mammalian U2 and U6 small nuclear ribonucleoprotein particles. Genes Dev. 4:2146-2156
30. Hodges, P. E., and J. D. Beggs. 1994. U2 fulfills a commitment. Curr. Biol. 4:264-267.
31. Huffaker, T. C., M. A. Hoyt, and D. Botstein. 1987. Genetic analysis of the yeast cytoskeleton. Annu Rev. Genet. 21:259-284.
32. Igel, A. H., and M. Ares, Jr. 1988. Internal sequences that distinguish yeast from metazoan U2 are unnecessary for pre-mRNA splicing. Nature (London) 334:450-453.
33. Ito, H., Y. Fakuda, K. Murata, and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163-168.
34. Jacquier, A., J. R. Rodriguez, and M. Rosbash. 1985. A quantitative analysis of the effects of 5′ junction and TACTAAC box mutants and mutant combinations on yeast mRNA splicing. Cell 43:423-430.
35. Jandrositz, A., and C. Guthrie. 1995. Evidence for a Prp24 binding site in U6 snRNA and in a putative intermediate in the annealing of U6 and U4 snRNAs. EMBO J 14:820-832.
36. Kandels-Lewis, S., and B. Seraphin. 1993. Role of U6 snRNA in 5′ splice site selection. Science 262:2035-2039.
37. Kunkel, T. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82:488-492
38. Legrain, P., C. Chapon, and F. Galisson. 1993. Interactions between PRP9 and SPP91 splicing factors identify a protein complex required in prespliceosome assembly. Genes Dev. 7:1390-1399
39. Lesser, C. F., and C. Guthrie. 1993. Mutations in U6 snRNA that alter splice site specificity: implications for the active site. Science 262:1982-1988.
40. Lin, R.-J., A. J. Lustig, and J. Abelson. 1987. Splicing of yeast nuclear pre-mRNA in vitro requires a functional 40S spliceosome and several extrinsic factors. Genes Dev. 1:7-18
41. Madhani, H. D., R. Bordonne, and C. Guthrie. 1990. Multiple roles for U6 snRNA in the splicing pathway Genes Dev. 4:2264-2277.
42. Madhani, H. D., and C. Guthrie. 1992. A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome. Cell 17:803-817.
43. Madhani, H. D., and C. Guthrie. 1994. Randomization-selection analysis of snRNAs in vivo, evidence for a tertiary interaction in the spliceosome Genes Dev. 8:1071-1086.
44. Madhani, H. D., and C. Guthrie. 1994. Dynamic RNA-RNA interactions in the spliceosome. Annu. Rev. Genet. 28:1-26.
45. McPheeters, D.S., and J. Abelson. 1992. Mutational analysis of the yeast U2 snRNA suggests a structural similarity to the catalytic core of group I introns. Cell 71:819-831.
46. Miller, A. 1984. The yeast MATa1 gene contains two introns. EMBO J. 3: 1061-1065.
47. Miraglia, L., S. Steiwert, A. H. Igel, and M. Ares, Jr. 1991. Limited functional equivalence of phylogenetic variation in small nuclear RNA: yeast U2 RNA with altered branchpoint complementarity inhibits splicing and produces a dominant lethal phenotype. Proc. Natl. Acad. Sci. USA 88:7061-7065.
48. Moore, M. J., C. C. Query, and P. A. Sharp. 1993. Splicing of precursors to mRNA by the spliceosome, p. 303-357. In R. Gesteland and J. Atkins (ed.), The RNA world. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
49. Newman, A. 1994. Small nuclear RNAs and pre-mRNA splicing. Curr. Opin. Cell Biol. 6:360-367
50. Nilsen, T. W. 1994. RNA-RNA interactions in the spliceosome: unraveling the ties that bind. Cell 78:1-4.
51. Orum, H., H. Nielsen, and J. Engberg. 1991. Spliceosomal small nuclear RNAs of Tetrahymena thermophila and some possible snRNA-snRNA base-pairing interactions. J. Mol. Biol. 222:219-232
52. Parker, R. A., P. G. Siliciano, and C. Guthrie. 1987 Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA Cell 49:229-239.
53. Ruby, S. W., T.-H. Chang, and J. Abelson. 1993. Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA. Genes Dev. 7:1909-1925.
54. Sawa, H., and J. Abelson. 1992. Evidence for a base-pairing interaction between U6 small nuclear RNA and 5′ splice site during the splicing reaction in yeast. Proc. Natl. Acad. Sci. USA 89:11269-11273.
55. Shannon, K. W., and C. Guthrie. 1991. Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motif. Genes Dev 5:773-785.
56. Sharp, P. A. 1985. On the origin of RNA splicing and introns. Cell 42: 397-400
57. Sherman, F., G. Fink, and J. Hicks. 1986. Methods in yeast genetics Cold Spring Harbor Laboratory, Cold Spring Harbor, N Y
58. Shuster, E. O., and C. Guthrie. 1988. Two conserved domains of yeast U2 snRNA are separated by 945 nonessential nucleotides. Cell 55:41-48
59. Staknis, D., and R. Reed. 1994. Direct interactions between pre-mRNA and six U2 small nuclear ribonucleoproteins during spliceosome assembly. Mol. Cell. Biol. 14:2994-3005.
60. Sun, J., and J. L. Manley. 1994. A novel U2-U6 snRNA structure is necessary for mammalian mRNA splicing. Genes Dev. 9:843-854.
61. Takahashi, Y., S. Urushiyama, T. Tani, and Y. Ohshima. 1993. An mRNA-type intron is present in the Rhodotorula hasegawae U2 small nuclear RNA gene. Mol. Cell. Biol. 13:5613-5619.
62. Vankan, P., C. McGuigan, and I. W. Mattaj. 1990. Domains of U4 and U6 snRNAs required for snRNP assembly and splicing complementation in Xenopus oocytes. EMBO J. 9:3397-3404.
63. Wassarman, D. A., and J. A. Steitz. 1992. Interactions of small nuclear RNAs with precursor messenger RNA during in vitro splicing Science 257:1918-1925.
64. Wells, S. E., and Ares, M., Jr. 1994. Interactions between highly conserved U2 small nuclear RNA structures and Prp5p, Prp9p, Prp11p, and Prp21p proteins are required to ensure integrity of the U2 small nuclear ribonuleoprotein in Saccharomyces cerevisiae. Mol. Cell. Biol. 14:6337-6349.
65. Wells, S. E., M. Neville, M. Haynes, J. Wang, H. Igel, and M. Ares, Jr. CUS1. a suppressor of cold sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP145. Genes Dev., in press.
66. Wolff, T., R. Menssen, J. Hammel, and A. Bindereif. 1994. Splicing function of mammalian U6 small nuclear RNA: conserved positions in central domain and helix I are essential during the first and second step of pre-mRNA splicing. Proc. Natl. Acad Sci USA 91:903-907.
67. Wu, J., and J. L. Manley. 1989. Mammalian pre-mRNA branch site selection by U2 snRNP involves base pairing. Genes Dev 3:1553-1561.
68. Wu, J., and J. L. Manley. 1991. Base pairing between U2 and U6 snRNs is necessary for splicing of a mammalian pre-mRNA. Nature (London) 352: 818-821.
69. Wu, J., and J. L. Manley. 1992. Multiple functional domains of human U2 small nuclear RNA: strengthening conserved stem I can block splicing. Mol. Cell. Biol. 12:5464-5473.
70. Zavanelli, M., J. Britton, H. Igel, and M. Ares, Jr. 1994. Mutations in an essential U2 small nuclear RNA structure cause cold sensitive U2 small nuclear ribonucleoprotein function by favoring competing U2 RNA structures Mol. Cell. Biol. 14:1689-1697.
71. Zavanelli, M. I., and M. Ares, Jr. 1991. Efficient association of U2 snRNPs with pre-mRNA requires an essential U2 RNA structural element. Genes Dev. 5:2521-2533.
72. Zhuang, Y., and A. M. Weiner. 1989. A compensatory base change in human U2 snRNA can suppress a branch site mutation. Genes Dev. 3:1545-1552.