Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing

EMBO Journal

Volumn 17, Issue 7, 1998, Pages 2086-2094

  Schwer, Beate ^a   Gross, Christian H ^a,b  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

^b MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

Author keywords

ATPase; DExH box; Prp22; RNA helicase; Spliceosome

Indexed keywords

ACTIN; ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATE; PROTEIN PRP22; RECOMBINANT PROTEIN; RNA HELICASE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN PURIFICATION; RNA SPLICING; SPLICEOSOME; YEAST;

ACTINS; ADENOSINE TRIPHOSPHATASES; ADENOSINE TRIPHOSPHATE; DEAD-BOX RNA HELICASES; FUNGAL PROTEINS; RECOMBINANT FUSION PROTEINS; RNA HELICASES; RNA NUCLEOTIDYLTRANSFERASES; RNA PRECURSORS; RNA SPLICING; RNA, FUNGAL; SACCHAROMYCES CEREVISIAE PROTEINS; SPLICEOSOMES; YEASTS;

EID: 0032055765     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.7.2086     Document Type: Article

References (55)

1. Abovich,N. and Rosbash,M. (1997) Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals. Cell, 89, 403-412.
2. Ansari,A. and Schwer,B. (1995) SLU7 and a novel activity, SSF1, act during the PRP16-dependent step of yeast pre-mRNA splicing. EMBO J., 14, 4001-4009.
3. Arenas,J.E. and Abelson,J.N. (1997) Prp43: An RNA helicase-like factor involved in spliceosome disassembly. Proc. Natl Acad. Sci. USA, 94, 11798-11802.
4. Arning,S., Grüter,P., Bilbe,G. and Krämer,A. (1996) Mammalian splicing factor SF1 is encoded by variant cDNAs and binds to RNA. RNA, 2, 794-810.
5. Berglund,J.A., Chua,K., Abovich,N., Reed,R. and Rosbash,M. (1997) The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC. Cell, 89, 781-787.
6. Brys,A. and Schwer,B. (1996) Requirement for SLU7 in yeast pre-mRNA splicing is dictated by the distance between the branchpoint and the 3′ splice site. RNA, 2, 707-717.
7. Burgess,S., Couto,J.R. and Guthrie,C. (1990) A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing. Cell, 60, 705-717.
8. Chen,J.H. and Lin,R.J. (1990) The yeast PRP2 protein, a putative RNA-dependent ATPase, shares extensive sequence homology with two other pre-mRNA splicing factors. Nucleic Acids Res., 18, 6447.
9. Company,M., Arenas,J. and Abelson,J. (1991) Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomes. Nature, 349, 487-493.
10. Conway,T.W. and Lipmann,F. (1964) Characterization of a ribosome-linked guanosine triphosphatase in Escherichia colt extracts. Proc. Natl Acad. Sci. USA, 52, 1462-1469.
11. Frank,D. and Guthrie,C. (1992) An essential splicing factor, SLU7, mediates 3′ splice site choice in yeast. Genes Dev., 6, 2112-2124.
12. Frank,D., Patterson,B. and Guthrie,C. (1992) Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing. Mol. Cell. Biol., 12, 5197-5205.
13. Fromont-Racine,M., Rain,J.C. and Legrain,P. (1997) Towards a functional analysis of the yeast genome through exhaustive two-hybrid screens. Nature Genet., 16, 277-282.
14. Goodwin,G.H. and Dahlberg,A.E. (1982) Electrophoresis of nucleo-proteins. In Rickwood, D. and Hames, B.D. (eds), Gel Electrophoresis of Nucleic Acids. IRL Press, Oxford, pp. 199-225.
15. Gross,C.H. and Shuman,S. (1995) Mutational analysis of vaccinia virus nucleoside triphosphate phosphohydrolase II, a DExH box RNA helicase. J. Virol., 69, 4727-4736.
16. Guthrie,C. (1991) Messenger RNA splicing in yeast: Clues to why the spliceosome is a ribonucleoprotein particle. Science, 253, 157-163.
17. Horowitz,D.S. and Abelson,J. (1993a) Stages in the second reaction of pre-mRNA splicing: the final step is ATP independent. Genes Dev., 7, 320-329.
18. Horowitz,D.S. and Abelson,J. (1993b) A U5 small nuclear ribonucleoprotein particle protein involved only in the second step of pre-mRNA splicing in Saccharomyces cerevisiae. Mol Cell. Biol., 13, 2959-2970.
19. Jones,M.H., Frank,D.N. and Guthrie,C. (1995) Characterization and functional ordering of Slu7p and Prp17p during the second step of pre-mRNA splicing in yeast. Proc. Natl Acad. Sci. USA, 92, 9687-9691.
20. Kim,S.H. and Lin,R.J. (1993) Pre-mRNA splicing within an assembled yeast spliceosome requires an RNA- dependent ATPase and ATP hydrolysis. Proc. Natl Acad. Sci. USA, 90, 888-892.
21. Kim,S.H. and Lin,R.J. (1996) Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing. Mol. Cell. Biol., 16, 6810-6819.
22. Kim,S.H., Smith,J., Claude,A. and Lin,R.J. (1992) The purified yeast pre-mRNA splicing factor PRP2 is an RNA-dependent NTPase. EMBO J., 11, 2319-2326.
23. Krämer,A. (1996) The structure and function of proteins involved in mammalian pre-mRNA splicing. Annu. Rev. Biochem., 65, 367-409.
24. Kuroda,M.I., Kernan,M.J., Kreber,R., Ganetzky,B. and Baker,B.S. (1991) The maleless protein associates with the X chromosome to regulate dosage compensation in Drosophila. Cell, 66, 935-947.
25. Lee,C.G. and Hurwitz,J. (1992) A new RNA helicase isolated from HeLa cells that catalytically translocates in the 3′ to 5′ direction. J, Biol. Chem., 267, 4398-4407.
26. Lee,C.G. and Hurwitz,J. (1993) Human RNA helicase A is homologous to the maleless protein of Drosophila. J. Biol. Chem., 268, 16822-16830.
27. Lee,C.G., Chang,K.A., Kuroda,M.I. and Hurwitz,J. (1997) The NTPase/helicase activities of Drosophila maleless, an essential factor in dosage compensation. EMBO J. 16, 2671-2681.
28. Lin,R.J., Newman,A.J., Cheng,S.C. and Abelson,J. (1985) Yeast mRNA splicing in vitro. J. Biol. Chem., 260, 14780-14792.
29. Lin,R.J., Lustig,A.J. and Abelson,J. (1987) Splicing of yeast nuclear pre-mRNA in vitro requires a functional 40S spliceosome and several extrinsic factors. Genes Dev., 1, 7-18.
30. Madhani,H.D. and Guthrie,C. (1994) Dynamic RNA-RNA interactions in the spliceosome. Annu. Rev. Genet., 28, 1-26.
31. Moore,M.J., Query,C.C. and Sharp,P.A. (1993) Splicing of precursors to mRNA by the spliceosome. In Gesteland, R.F. and Atkins, J.F. (eds), The RNA World. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 303-357.
32. Newman,A.J. (1997) The role of U5 snRNP in pre-mRNA splicing. EMBO J., 16, 5797-5800.
33. Newman,A.J. and Norman,C. (1992) U5 snRNA interacts with exon sequences at 5′ and 3′ splice sites. Cell, 68, 743-754.
34. Newman,A.J., Teigelkamp,S. and Beggs,J.D. (1995) snRNA interactions at 5′ and 3′ splice sites monitored by photoactivated crosslinking in yeast spliceosomes. RNA, 1, 968-980.
35. Ng,R. and Abelson,J. (1980) Isolation and sequence of the gene for actin in Saccharomyces cerevisiae. Proc. Natl Acad. Sci. USA, 77, 3912-3916.
36. Nilsen,T.W. (1994) RNA-RNA interactions in the spliceosome: unraveling the ties that bind. Cell. 78, 1-4.
37. Noble,S.M. and Guthrie,C. (1996) Identification of novel genes required for yeast pre-mRNA splicing by means of cold-sensitive mutations. Genetics, 143, 67-80.
38. Ohno,M. and Shimura,Y. (1996) A human RNA helicase-like protein, HRH1, facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. Cenex Dev., 10, 997-1007.
39. Ono,Y., Ohno,M. and Shimura,Y. (1994) Identification of a putative RNA helicase (HRH1), a human homolog of yeast Prp22. Mol. Cell. Biol, 14, 7611-7620.
40. Plumpton,M., McGarvey,M. and Beggs,J.D. (1994) A dominant negative mutation in the conserved RNA helicase motif 'SAT' causes splicing factor PRP2 to stall in spliceosomes. EMBO J., 13, 879-887.
41. Ruby,S.W. and Abelson,J. (1991) Pre-mRNA splicing in yeast. Trends Genet., 7, 79-85.
42. Rymond,B.C., Torrey,D.D. and Rosbash,M. (1987) A novel role for the 3′ region of introns in pre-mRNA splicing of Saccharomyces cerevisiae. Genes Dev., 1, 238-246.
43. Sawa,H. and Shimura,Y. (1991) Requirement of protein factors and ATP for the disassembly of the spliceosome after mRNA splicing reaction. Nucleic Acids Res., 19, 6819-6821.
44. Schmid,S.R. and Linder,P. (1992) D-E-A-D protein family of pulative RNA helicases. Mol. Microbiol. 6, 283-291.
45. Schwer,B. and Guthrie,C. (1991) PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome. Nature, 349, 494-499.
46. Schwer,B. and Guthrie,C. (1992) A conformational rearrangement in the spliceosome is dependent on PRP16 and ATP hydrolysis. EMBO J., 11, 5033-5039.
47. Shuman,S. (1992) Vaccinia virus RNA helicase: an essential enzyme related to the DE-H family of RNA-dependent NTPases. Proc. Natl Acad. Sci. USA, 89, 10935-10939.
48. Sontheimer,E.J. and Steitz,J.A. (1993) The U5 and U6 small nuclear RNAs as active site components of the spliceosome [see comments] [published erratum appears in Science (1994) Feb 11; 263(5148), 739]. Science, 262, 1989-1996.
49. Teigelkamp,S., McGarvey,M., Plumpton,M. and Beggs,J.D. (1994) The splicing factor PRP2, a putative RNA helicase, interacts directly with pre-mRNA. EMBO J., 13, 888-897.
50. Teigelkamp,S., Newman,A.J. and Beggs,J.D. (1995) Extensive interactions of PRP8 protein with the 5′ and 3′ splice sites during splicing suggest a role in stabilization of exon alignment by U5 snRNA, EMBO J., 14, 2602-2612.
51. Umen,J.G. and Guthrie,C. (1995a) Prp16p, Slu7p, and Prp8p interact with the 3′ splice site in two distinct stages during the second catalytic step of pre-mRNA splicing. RNA, 1, 584-597.
52. Umen,J.G. and Guthrie,C. (1995b) The second catalytic step of pre-mRNA splicing. RNA. 1, 869-885.
53. Vijayraghavan,U., Parker,R., Tamm,J., Iimura,Y., Rossi,J., Abelson,J. and Guthrie,C. (1986) Mutations in conserved intron sequences affect multiple steps in the yeast splicing pathway, particularly assembly of the spliceosome. EMBO J., 5, 1683-1695.
54. Vijayraghavan,U., Company,M. and Abelson,J. (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev., 3, 1206-1216.
55. Zhang,X. and Schwer,B. (1997) Functional and physical interaction between the yeast splicing factors Slu7 and Prp18. Nucleic Acids Res., 25, 2146-2152.