Yeast ribosomes bind to highly purified reconstituted Sec61p complex and to mammalian p180

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Volumn 2, Issue 10, 2001, Pages 705-716

  Morrow, Michael W ^a   Brodsky, Jeffrey L ^a  

^a UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

Endoplasmic reticulum; p180; SEC61; Translocation; Yeast

Indexed keywords

HISTIDINE; LIPOSOME; PROTEIN P180; RIBOSOME PROTEIN; SEC61P PROTEIN; SEC63P P ROTEIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; COMPLEX FORMATION; CONTAMINATION; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; GENE EXPRESSION; IN VIVO STUDY; MAMMAL; MICROSOME; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN ISOLATION; PROTEIN TRANSPORT; PURIFICATION; RECEPTOR AFFINITY; RIBOSOME; RNA TRANSLATION; SCATCHARD PLOT; SEQUENCE TAGGED SITE; YEAST;

ANIMALS; ENDOPLASMIC RETICULUM; FUNGAL PROTEINS; GENE EXPRESSION; HEAT-SHOCK PROTEINS; MAMMALS; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; PEPTIDE ELONGATION FACTOR 1; PROTEIN BINDING; PROTEIN TRANSPORT; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RIBOSOMES; SACCHAROMYCES CEREVISIAE PROTEINS; YEASTS;

EID: 0034794820     PISSN: 13989219     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1600-0854.2001.21005.x     Document Type: Article

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5. Regulation of ribosome detachment from the mammalian endoplasmic reticulum membrane
6. Protein translocation into proteoliposomes reconstituted from purified components of the endoplasmic reticulum
7. Binding of ribosomes to the rough endoplasmic reticulum mediated by the Sec61p-complex
8. Posttranslational protein transport in yeast reconstituted with a purified complex of Sec proteins and Kar2p
9. A second trimeric complex containing homologs of the Sec61p complex functions in protein transport across the ER membrane of S. cerevisiae
10. Identification of a ribosome receptor in the rough endoplasmic reticulum
11. 180-kD ribosome receptor is essential for both ribosome binding and protein translocation
12. Functional characterization of the 180-kD ribosome receptor in vivo
13. Identification of a membrane protein responsible for ribosome binding in rough microsomal membranes
14. Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation
15. The aqueous pore through the translocon has a diameter of 40-60 Å during cotranslational protein translocation at the ER membrane
16. Alignment of conduits for the nascent polypeptide chain in the ribosome-Sec61 complex
17. The structure of ribosome-channel complexes engaged in protein translocation
18. Evolutionary conservation of components of the protein translocation complex
19. Protein translocation mutants defective in the insertion of integral membrane proteins into the endoplasmic reticulum
20. A yeast mutant defective at an early stage in import of secretory protein precursors into the endoplasmic reticulum
21. Yeast Sec proteins interact with polypeptides traversing the endoplasmic reticulum membrane
22. Sec61p and BiP directly facilitate polypeptide translocation into the ER
23. The yeast SSS1 gene is essential for secretory protein translocation and encodes a conserved protein of the endoplasmic reticulum
24. Expression of the p180-kD ribosome receptor induces membrane proliferation and increased secretory activity in yeast
25. Reconstitution of protein translocation from solubilized yeast membranes reveals topologically distinct roles for BiP and cytosolic Hsc70
26. Numbers of receptor sites from Scatchard graphs: Facts and fantasies
27. The β subunit of the Sec61 complex facilitates cotranslational protein transport and interacts with the signal peptidase during translocation
28. Evolutionarily conserved binding of ribosomes to the translocation channel via the large ribosomal RNA
29. Role of the cytoplasmic segments of Sec61α in the ribosome-binding and translocation-promoting activities of the Sec61 complex
30. Sec61p is the main ribosome receptor in the endoplasmic reticulum of Saccharomyces cerevisiae
31. Site-directed mutants of post translationally modified sites of yeast eEF1A using a shuttle vector containing a chromogenic switch
32. Signal sequences specify the targeting route to the endoplasmic reticulum membrane
33. Loss of BiP/GRP78 function blocks translocation of secretory proteins in yeast
34. Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast
35. SEC62 encodes a putative membrane protein required for protein translocation into the yeast endoplasmic reticulum
36. Sec63p-BiP complex from yeast is required for protein translocation in a reconstituted proteoliposome
37. BiP and Sec63p are required for both co- and posttranslational protein translocation into the yeast endoplasmic reticulum
38. Interaction between BiP and Sec63p is required for the completion of protein translocation into the ER of Saccharomyces cerevisiae
39. BiP acts as a molecular ratchet during posttranslational transport of prepro-alpha factor across the ER membrane
40. SEC11 is required for signal peptide processing and yeast cell growth
41. Sequence-specific alteration of the ribosome membrane junction exposes nascent secretory proteins to the cytosol
42. Regulation of the ribosome-membrane junction at early stages of presecretory protein translocation in the mammalian endoplasmic reticulum
43. TRAM regulates the exposure of nascent secretory proteins to the cytosol during translocation into the endoplasmic reticulum
44. Binding of secretory precursor polypeptides to a translocon subcomplex is regulated by BiP
45. Signal sequence recognition in posttranslational protein transport across the yeast ER membrane
46. Detection of transient in vivo interactions between substrate and transporter during protein translocation into the endoplasmic reticulum
47. A phosphatidylinositol transfer protein controls the phosphatidylcholine content of yeast Golgi membranes
48. Identification of a novel stage of ribosome/nascent chain association with the endoplasmic reticulum membrane
49. Mutations in a GTP-binding motif of eukaryotic elongation factor 1A reduce both translational fidelity and the requirement of nucleotide exchange
50. Translational misreading mutations in translation elongation factor 1α differentially affect programmed ribosomal frameshifting and drug sensitivity
51. Rapid degradation of a large fraction of newly synthesized proteins by proteosomes
52. Distinct domains within yeast Sec61p involved in post-translational translocation and protein dislocation
53. Cloning of SEC61 homologues from Schizosaccharomyces pombe and Yarrowia lipolytica reveals the extent of functional conservation within this core component of the ER translocation machinery
54. Sec61p mediates export of a misfolded secretory protein from the endoplasmic reticulum to the cytosol for degradation
55. A yeast gene important for protein assembly in the endoplasmic reticulum and the nucleus has homology to DnaJ, an Escherichia coli heat shock protein
56. The ribosomal proteins of Saccharomyces cerevisiae
57. Yeast ribosomal protein L1 is required for the stability of newly synthesized 5S rRNA and the assembly of 60S ribosomal subunits
58. Selective radiolabeling of cell surface proteins to a high specific activity
59. Preparation of microsomal membranes for cotranslational protein translocation
60. Quantitation of membrane proteins in reconstituted vesicles prepared from yeast
61. Regulation of Hsp70 function by a eukaryotic DnaJ homolog
62. Quantitative and qualitative analysis of lipids and lipid components
63. Mutations in the cytosolic DnaJ homologue, YDJ1, delay and compromise the efficient translation of heterologous proteins in yeast